Divergence of secondary metabolism in cell suspension cultures and differentiated plants of Piper cernuum and P. crassinervium

Danelutte, Ana Paula; Costantin, Mara B.; Delgado, Guillermo E.; Braz-Filho, Raimundo; Kato, Massuo Jorge

doi:10.1590/s0103-50532005000800022

Cited by 44 publications

(31 citation statements)

References 29 publications

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“…In order to obtain high yields suitable for commercial exploitation, efforts have focused on studying the biosynthetic pathways of biologically active compounds through cell suspension culture (Dicosmo & Misawa 1995). Recently, Danelutte et al (2005) carried out the only study aimed at establishing cell suspension cultures in Piperaceae and an investigation of the associated secondary metabolites in Piper cernuum and Piper crassinervium.…”

Section: Introductionmentioning

confidence: 99%

In vitro morphogenesis and cell suspension culture establishment in Piper solmsianum C. DC. (Piperaceae)

et al. 2009

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RESUMO -(Morfogênese in vitro e estabelecimento de culturas de suspensão celular em Piper solmsianum C. DC. (Piperaceae)). Piper solmsianum é uma espécie herbácea do sudeste brasileiro onde vários compostos biologicamente ativos já foram identificados. O objetivo deste trabalho foi estabelecer suspensões celulares nesta espécie. Para tanto, foram utilizados explantes de pecíolos e folhas, retirados de plântulas cultivadas in vitro, os quais foram submetidos a diferentes combinações de reguladores de crescimento (AIA, ANA, 2,4-D e BAP). Foi obtida a neo-formação de raízes e brotos, estes últimos através do processo de organogênese indireta evidenciada por estudos histológicos. Para a indução e crescimento dos calos, foram avaliados, além das diferentes combinações de reguladores de crescimento, a suplementação ao meio de cultura de carvão ativado (1,5 mg.l -1 ) e o regime de luz. Culturas mantidas na luz, em meio de cultura suplementado com 0,2 mg.l -1 2,4-D e 2 mg.l -1 BAP e sem carvão ativado, resultaram em maior crescimento (massa fresca) dos calos. A partir destes calos, foram obtidas suspensões celulares, cuja dinâmica de crescimento e acúmulo de metabólitos foi estudado. Os resultados obtidos deverão ser utilizados para a caracterização de rotas biosintéticas em culturas in vitro em P. solmsianum. Palavras-chave: calogênese, morfogênese, pariparoba, reguladores de crescimento, suspensões celulares ABSTRACT -(In vitro morphogenesis and cell suspension culture establishment in Piper solmsianum C. DC. (Piperaceae)).Piper solmsianum is a shrub from Southeast Brazil in which many biologically active compounds were identified. The aim of this work was to establish a cell suspension culture system for this species. With this in mind, petiole and leaf explants obtained from in vitro plantlets were cultured in the presence of different plant growth regulator combinations (IAA, NAA, 2,4-D and BA). Root and indirect shoot adventitious formation, detected by histological analysis, was observed. Besides the different combinations of plant growth regulators, light regime and the supplement of activated charcoal (1.5 mg.l -1 ) were tested for callus induction and growth. Cultures maintained in light, on a 0.2 mg.l -1 2,4-D and 2 mg.l -1 BA supplemented medium, and in the absence of activated charcoal, showed the highest calli fresh matter increment. From a callus culture, cell suspension cultures were established and their growth and metabolite accumulation studied. The achieved results may be useful for further characterization of the activated secondary metabolites pathways in in vitro systems of P. solmsianum.

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Section: Introductionmentioning

confidence: 99%

In vitro morphogenesis and cell suspension culture establishment in Piper solmsianum C. DC. (Piperaceae)

et al. 2009

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“…Plants from the genus Piper are widely distributed throughout tropical and subtropical regions. Brazil has several species and promising studies on biological properties of plants of the Piperaceae family have been published (Navickiene et al, 2000;Danelutte et al, 2003Danelutte et al, , 2005Cysne et al, 2005;Yamaguchi et al, 2006).…”

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confidence: 99%

Molluscicidal and ovicidal activities of plant extracts of the Piperaceae on Biomphalaria glabrata (Say, 1818)

et al. 2010

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Schistosomiasis is a tropical disease caused by Schistosoma and occurs in 54 countries, mainly in South America, the Caribbean region, Africa and the eastern Mediterranean. Currently, 5 to 6 million Brazilian people are infected and 30,000 are under infection risk. Typical of poor regions, this disease is associated with the lack of basic sanitation and very frequently to the use of contaminated water in agriculture, housework and leisure. One of the most efficient methods of controlling the disease is application of molluscicides to eliminate or to reduce the population of the intermediate host snail Biomphalaria glabrata. Studies on molluscicidal activity of plant extracts have been stimulated by issues such as environmental preservation, high cost and recurrent resistance of snails to synthetic molluscicides. The aim of this study was to determine the molluscicide action of extracts from Piperaceae species on adult and embryonic stages of B. glabrata. Fifteen extracts from 13 Piperaceae species were obtained from stems, leaves and roots. Toxicity of extracts was evaluated against snails at two different concentrations (500 and 100 ppm) and those causing 100% mortality at 100 ppm concentration were selected to obtain the LC 90 (lethal concentration of 90% mortality). Piper aduncum, P. crassinervium, P. cuyabanum, P. diospyrifolium and P. hostmannianum gave 100% mortality of adult snails at concentrations ranging from 10 to 60 ppm. These extracts were also assayed on embryonic stages of B. glabrata and those from P. cuyabanum and P. hostmannianum showed 100% ovicidal action at 20 ppm.

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“…Leaf explants were individually transferred with the adaxial surface up, to test tubes (25 mm x 150 mm) containing 10 mL of an MS (Murashige & Skoog, 1962) basal culture medium supplemented with 3% (w/v) sucrose, 0.6% (w/v) agar and factorial combinations of 2,4-Dichlorophenoxyacetic acid (2,4-D) (0, 4.52, 9.05, or 18.10 µM) and 6-Benzylaminopurine (BA) (0, 4.44, 8.88, or 17.76 (Table 1) each one with three replicates of three explants. The growth regulators, their concentrations and combinations tested were based on successful studies on callus induction in Piper species; P. cernuum, P. crassinervum (Danelutte et al, 2005), P. solmsianum (Balbuena et al, 2009), P. aduncum (Delgado-Paredes et al, 2013), and P. hispidinervum (Santiago, 2013). The pH of the medium was adjusted to 5.8 before the addition of agar followed by autoclaving at 121°C for 20 minutes.…”

Section: Methodsmentioning

confidence: 99%

“…Danelutte et al (2005), aiming at the establishment of cell suspensions from leaves of P. crassinervium and P. cernuum, found the highest callus induction by using MS medium supplemented with 0.11 µM Indoleacetic acid (IAA) and 0.06 µM GA 3 in P. crassinervium and 0.91 µM 2,4-D and 1.44 µM GA 3 in P. cernuum. The last combination was also tested in the current approach, without success.…”

Section: Callus Inductionmentioning

confidence: 99%

“…Several species of the Piper genus are native to the Amazon Rainforest and are notable producers of chemical compounds which have several biological effects in insects, fungi, bacteria, Trypanossoma cruzi (Navickiene et al, 2003;Dyer et al, 2004;Danelutte et al, 2005;Balbuena et al, 2009) and can also affect human health, as analgesic, anti-depressant, cytoprotective, anti-ulcer, anticonvulsant, anti-inflammatory, and antioxidant agents (Ahmad et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Induction and growth pattern of callus from Piper permucronatum leaves

Santos

Guimarães

Paz

et al. 2016

Rev. bras. plantas med.

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ABSTRACT:Piper permucronatum is a perennial shrub, a medicinal plant native to the Amazon Rainforest. Traditionally, the tea of its leaves is used to combat menstrual and intestinal cramps, stomach pain, digestive problems, diarrhea, hemorrhage, and nausea. Its leaf's essential oil is effective against Aedes aegypti larvae; its flavones and flavanones have a fungicidal effect against Clamidosporium cladosporioides and C. sphaerospermum; its hexanic extract is effective against Leishmania amazonensis. The objective of this study was to provide a protocol for callus induction from P. permucronatum leaves and an identification of the callus growth pattern, focusing on the deceleration phase, when the callus cells must be subcultured into liquid medium in order to produce a cell suspension cultures. Leaf explants were inoculated in a solid MS medium supplemented with factorial combinations of 2,4-D, BA, NAA and GA 3 . Callus formation was evaluated weekly until the 49 th day. Subsequently, new explants were inoculated at the hormonal combination that resulted in the highest callus cell proliferation and, every seven days during a period of 70 days, samples were dried and weighed to determine the callus growth pattern. NAA and GA 3 were not effective for callus induction. Combinations of 2,4-D and BA resulted in callus induction and proliferation. The highest percentage of callus induction was observed with the combination of 4.52 µM 2,4-D and 4.44 µM BA. The calluses thereby produced were friable and whitish. The callus growth pattern followed a sigmoid shape. The deceleration phase started on the 56 th day of culture.Keywords: Piperaceae, growth regulators, callogenesis.RESUMO: Indução e padrão de crescimento de calos de folhas de Piper permucronatum. Indução e padrão de crescimento de calos de folhas de Piper permucronatum. Piper permucronatum é um arbusto perene, uma planta medicinal native da Floresta Amazônica. Tradicionalmente, o chá de suas folhas é usado em casos de cólicas menstruais e intestinais, dores de estômago, problemas digestivos, diarreia, hemorragia e náusea. O óleo essencial das folhas é efetivo contra a larva de Aedes aegypti; suas flavonas e flavanonas têm efeito fungicida contra Clamidosporium cladosporioides e C. sphaerospermum; seu extrato hexânico é efetivo contra Leishmania amazonensis. O objetivo deste trabalho foi determinar um protocolo para indução de calos em folhas de P. permucronatum e identificar o padrão de crescimento dos calos, com foco na fase de desaceleração, quando as células de calo devem ser subcultivadas em meio líquido para produzir culturas de células em suspensão. Explantes foliares foram inoculados em meio MS sólido suplementado com combinações fatoriais de 2,4-D, BAP, ANA e GA 3 . A formação de calos foi avaliada semanalmente até o 49º dia. Posteriormente, novos explantes foram inoculados na combinação hormonal que resultou na maior proliferação de células de calo e, a cada sete dias durante 70 dias, amostras foram secas e pesadas para determinar o padrão de crescime...

show abstract

Divergence of secondary metabolism in cell suspension cultures and differentiated plants of Piper cernuum and P. crassinervium

Cited by 44 publications

References 29 publications

In vitro morphogenesis and cell suspension culture establishment in Piper solmsianum C. DC. (Piperaceae)

In vitro morphogenesis and cell suspension culture establishment in Piper solmsianum C. DC. (Piperaceae)

Molluscicidal and ovicidal activities of plant extracts of the Piperaceae on Biomphalaria glabrata (Say, 1818)

Induction and growth pattern of callus from Piper permucronatum leaves

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