Keratinocyte growth factor and the expression of wound-healing-related genes in primary human keratinocytes from burn patients

Chomiski, Verônica; Gragnani, Alfredo; Bonucci, Jéssica; Correa, Silvana Aparecida Alves; Noronha, Samuel Marcos Ribeiro de; Ferreira, Lydia Masako

doi:10.1590/s0102-865020160080000002

Cited by 3 publications

(4 citation statements)

References 26 publications

(28 reference statements)

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“…In the years of women with reproductive capacity, normal endometrium undergoes about 400 cycles of self-repairing, including: shedding, regeneration, and differentiation and without scarring (Gargett, 2007 ). A general consensus has been reached that trauma and infection are the most common causes of IUA (Chomiski et al., 2016 ). At present, although clinical treatment using IUDs for IUA can restore the shape of the uterus cavity, it prevents normal endometrial regeneration and may increase risk of infection.…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, angiogenesis-enhancing cytokines and growth factors secreted by stem cell have received increasing attention. Keratinocyte growth factor (KGF), also known as fibroblastic growth factor-7 (FGF-7), is a stromally derived, secreted peptide that acts as a mitogen for epithelial cells in culture (Chomiski et al., 2016 ; Poorebrahim et al., 2017 ). In previous studies, KGF has been proved to facilitate regeneration and healing of wound in injured skin, oral mucosa, and cornea (Eming et al., 1999 ; Finch & Rubin, 2004 ).…”

Section: Introductionmentioning

confidence: 99%

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Temperature-sensitive heparin-modified poloxamer hydrogel with affinity to KGF facilitate the morphologic and functional recovery of the injured rat uterus

Zhang

et al. 2017

Drug Delivery

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Endometrial injury usually results in intrauterine adhesion (IUA), which is an important cause of infertility and recurrent miscarriage in reproductive women. There is still lack of an effective therapeutic strategy to prevent occurrence of IUA. Keratinocyte growth factor (KGF) is a potent repair factor for epithelial tissues. Here, a temperature-sensitive heparin-modified poloxamer (HP) hydrogel with affinity to KGF (KGF-HP) was used as a support matrix to prevent IUA and deliver KGF. The rheology of KGF-HP hydrogel was carefully characterized. The cold KGF-HP solution was rapidly transited to hydrogel with suitable storage modulus (G') and loss modulus (G″) for the applications of uterus cavity at temperature of 33 °C. In vitro release demonstrated that KGF was released from HP hydrogels in sustained release manner for a long time. In vivo bioluminescence imaging showed that KGF-HP hydrogel was able to prolong the retention of the encapsulated KGF in injured uterus of rat model. Moreover, the morphology and function of the injured uterus were significantly recovered after administration of KGF-HP hydrogel, which were evaluated by two-dimensional ultrasound imaging and receptive fertility. Not only proliferation of endometrial glandular epithelial cells and luminal epithelial cells but also angiogenesis of injured uterus were observed by Ki67 and CD31 staining after 7 d of treatment with KGF-HP hydrogel. Finally, a close relatively relationship between autophagy and proliferation of endometrial epithelial cells (EEC) and angiogenesis was firstly confirmed by detecting expression of LC3-II and P62 after KGF treatment. Overall, KGF-HP may be used as a promising candidate for IUA treatment.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Temperature-sensitive heparin-modified poloxamer hydrogel with affinity to KGF facilitate the morphologic and functional recovery of the injured rat uterus

Zhang

et al. 2017

Drug Delivery

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“…Chomiski and colleagues tested the impact of KGF treatment on the expression of wound-healing-related genes using keratinocyte cells culture obtained from burn patients. The study included keratinocyte cells divided into four groups, as follows: TKB (KGF-treated keratinocytes from burn patients), UKB (untreated keratinocytes from burn patients), TKC (KGF-treated keratinocytes from controls), and UKC (untreated keratinocytes from controls) and concluded that there is no difference between wound-healing-related genes expression [126]. Moreover, in corneal injury, KGF-2 manifests better effects in re-epithelialization, acceleration of migration, and reduction of scar formation, compared with bFGF [127].…”

Section: Keratinocyte Growth Factor and Wound Healingmentioning

confidence: 99%

Growth Factors, Reactive Oxygen Species, and Metformin—Promoters of the Wound Healing Process in Burns?

Miricescu

Bădoiu

Stănescu-Spînu

et al. 2021

IJMS

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Burns can be caused by various factors and have an increased risk of infection that can seriously delay the wound healing process. Chronic wounds caused by burns represent a major health problem. Wound healing is a complex process, orchestrated by cytokines, growth factors, prostaglandins, free radicals, clotting factors, and nitric oxide. Growth factors released during this process are involved in cell growth, proliferation, migration, and differentiation. Reactive oxygen species are released in acute and chronic burn injuries and play key roles in healing and regeneration. The main aim of this review is to present the roles of growth factors, reactive oxygen species, and metformin in the healing process of burn injuries.

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“…[ 5 , 6 ] Current studies have shown that growth factor has a good effect on tissue repair of burn wounds. [ 7 , 8 ]…”

Section: Introductionmentioning

confidence: 99%

Efficacy and safety of platelet-rich plasma in the treatment of severe burns

Chen

Turxun

et al. 2020

Medicine

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Background: In recent years, platelet-rich plasma (PRP) has been used in burn wound repair, plastic surgery, bone and tendon ligament injury repair and other treatment at home and abroad. Clinical studies indicate that PRP has a good curative effect on repairing burn wounds. The residual wounds formed after large area severe burns are difficult to cure and have recurrent attacks. Because the action mechanism of PRP is unclear, its clinical efficacy of PRP in repairing severe burn wounds is controversial. And there is no relevant systematic evaluation of PRP in repairing severe burn wounds now. Objective: Meta analysis was used to evaluate the efficacy and safety of PRP in repairing severe burn wounds. Methods: Randomized controlled clinical trial of using PRP to repair severe burn wounds were retrieved by computer WanFang, China National Knowledge Infrastructure, China Science and Technology Journal Database, China Biology Medicine disc, Embase, PubMed, Web of Science and Cochrane Library from the establishment of the database to September 2020. Two researchers independently extract data and assess the quality of the included literature, and Meta analysis of the included literature is carried out by using RevMan5.3 software. Results: In this study, the efficacy and safety of PRP in repairing severe burn wounds are evaluated from the aspects of wound healing rate, wound healing time, scar index, visual simulation score, the number of layers of dressing, the number of times of changing gauze, frequency of dressing change, the positive rate of wound bacterial culture and the incidence of inflammatory reaction. Conclusions: PRP has a good curative effect on the repair of severe burn wounds. This study provides reliable evidence for the clinical use of PRP in the clinical repair of severe burn wounds. OSF Registration number: DOI 10.17605/OSF.IO/FG682

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Keratinocyte growth factor and the expression of wound-healing-related genes in primary human keratinocytes from burn patients

Cited by 3 publications

References 26 publications

Temperature-sensitive heparin-modified poloxamer hydrogel with affinity to KGF facilitate the morphologic and functional recovery of the injured rat uterus

Temperature-sensitive heparin-modified poloxamer hydrogel with affinity to KGF facilitate the morphologic and functional recovery of the injured rat uterus

Growth Factors, Reactive Oxygen Species, and Metformin—Promoters of the Wound Healing Process in Burns?

Efficacy and safety of platelet-rich plasma in the treatment of severe burns

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