Study on the effect of black cumin (Nigella sativa Linn.) on experimental renal ischemia-reperfusion injury in rats

Abstract

PURPOSE:To evaluate the effect of Black cumin (Nigella sativa Linn.) pre-treatment on renal ischemia/reperfusion (I/R) induced injury in the rats. METHODS:A total of 40 male Wistar rats were randomly allocated into five equal groups including Sham, I/R model and three I/R+ Black cumin (0.5, 1 and 2%)-treated groups. I/R groups' kidneys were subjected to 60 min of global ischemia at 37°C followed by 24 h of reperfusion. At the end of reperfusion period, the rats were euthanized. Superoxide dismutase, catalase and glutathione peroxidase activities as well as reduced glutathione and renal malondialdehyde contents were determined in renal tissues. Kidney function tests and histopathological examination were also performed. RESULTS:High serum creatinine, blood urea nitrogen and uric acid as well as malondialhehyde (MDA) levels, and low antioxidant enzyme activities were observed in I/R rats compared to the sham rats. Pre-treatment with Black cumin for three weeks prior to IR operation improved renal function and reduced I/R induced renal inflammation and oxidative injury. These biochemical observations were supported by histopathological test of kidney sections. CONCLUSION:Black cumin significantly prevented renal ischemia/reperfusion induced functional and histological injuries.Key words: Nigella sativa. Ischemia. Reperfusion. Kidney. Rats. Study on the effect of black cumin (Nigella sativa Linn.) on experimental renal ischemia-reperfusion injury in rats

Protective Effect of Ginkgo biloba and Magnetized Water on Nephropathy in Induced Type 2 Diabetes in Rat

“…Absence of Ginkgo biloba hazard effects [ 49 ], low effect of high kidney related-serum parameters [ 50 , 51 ], renal injury attenuating effect [ 14 – 16 ], antioxidant effect [ 52 ] and fatty acid regulation effect [ 53 – 55 ], encouraged us to use it to decrease nephrotoxic effects in diabetic rats.…”

Th e eff ects of Nigella Sativa extract on renal tissue oxidative damage during neonatal and juvenile growth in propylthiouracil-induced hypothyroid rats

“…In recent years, an attention has been drawn to various plants and plant-derived compounds for the treatment of kidney diseases (Mousavi 2015;Mohebbati et al 2016). Among the promising medicinal plants, NS, commonly known as Kalonji in India and Pakistan, has been used traditionally for various ailments (Boskabady et al 2007;Qidwai et al 2009).…”

“…Many studies have shown that NS have anti-infl ammatory eff ect in various models (Boskabady et al 2008;Boskabady et al 2011a;Boskabady et al 2011b;Boskabady et al 2011c;Gholamnezhad et al 2015). It has been demonstrated that NS treatment increases CAT, GSH-Px, and SOD activities in the renal ischemia-reperfusion injury (Mousavi 2015). Th e results of another study have shown that NS oil may have protective eff ects against the hepatotoxicity and renal toxicity induced by ethanol through decreasing lipid peroxidation, infl ammation, and apoptosis (Pourbakhsh et al 2014).…”

“…Th erefore, fi ndings of our study showed that BUN and creatinine were decreased by NS therapy. Using a renal ischemia-reperfusion in-jury model, it has been demonstrated that the serum creatinine, BUN and uric acid levels were increased, which were prevented by NS administration (Mousavi 2015). In another study, NS oil ameliorated the sodium nitrite-induced nephrotoxicity via blocking the oxidative stress and reduced elevation of the serum urea and creatinine induced by sodium nitrite (AlGayyar et al 2016).…”

Use of Selected Antioxidant-Rich Spices and Herbs in Foods

“…Animal studies are available showing the hypolipidemic and antidiabetic effects of cumin [78]. Experimental studies have been conducted to support the effect of cumin on renal ischemia-reperfusion injury [79,80]. It also has antibacterial and potent antimicrobial activity [66,77].…”