Th e eff ects of Nigella Sativa extract on renal tissue oxidative damage during neonatal and juvenile growth in propylthiouracil-induced hypothyroid rats

Abstract

Objective. We investigated the eff ects of hydroalcoholic extract of Nigella sativa (NS) on renal tissue oxidative damage associated with propylthiouracil (PTU)-induced hypothyroidism during neonatal and juvenile growth in rats.Methods. Pregnant rats were divided into fi ve groups designated as: 1) control; 2) propylthiouracil (PTU); 3) PTU-NS100; 4) PTU-NS200, and 5) PTU-NS400. All mothers except the control group received 0.005% PTU in their drinking water during lactation. Besides PTU, mothers in groups 3-5 received 100, 200, and 400 mg/kg of NS extract. Aft er lactation period, the off spring continued to receive the same experimental treatment for the fi rst 8 weeks of their life. Ten male off springs of each group were randomly selected, blood samples collected, and the kidney tissues removed.Results. Th e serum thyroxin concentration in PTU group was lower than control group and improved by extract. PTU increased the renal malondialdehyde (MDA), while reduced the total thiols concentrations and catalase (CAT) and superoxide dismutase (SOD) activity compared to control group. Administration of 200 and 400 mg/kg of NS extract decreased MDA level, while it increased the total thiols and 400 mg/kg increased CAT and SOD activity in renal tissues compared to PTU group. Serum creatinine and blood urea nitrogen (BUN) in PTU group was higher than in comparison with the control group. 400 mg/kg decreased creatinine, but both 200 and 400 mg/kg improved BUN concentration compared to PTU group.Conclusion. Th e results of this study demonstrate that the hydroalcoholic extract of NS has a protective eff ect on the renal tissue oxidative damage associated with PTU-induced hypothyroidism during neonatal and juvenile growth in rats.

Thymoquinone Upregulates Catalase Gene Expression and Preserves the Structure of the Renal Cortex of Propylthiouracil-Induced Hypothyroid Rats

“…On the other hand, the current study demonstrated a significant upregulation of catalase gene expression in the kidney of the hypothyroid+TQ group, which was in an agreement with Khan et al [31]. Mohebbati et al [14] also reported an increase in CAT and SOD activity in renal tissues following administration of 200 and 400 mg/kg of N. sativa extract compared to the PTU group.…”

“…In addition, NS and TQ were recently reported to have the potential to prevent renal tissue damage and fibrosis in lipopolysaccharide-(LPS-) treated rats through its antioxidant and anti-inflammatory effects [12,13]. It was reported that the hydroalcoholic extract of NS could protect the renal tissue against oxidative stress associated with neonatal and juvenile hypothyroidism in rats [14] None of the available studies neither investigated the effect of the TQ in specific, the bioactive constituent of NS, on the structure of the renal tubules of hypothyroid animal model nor tried to explore the mechanism behind this effect. Therefore, the aim of this study was to evaluate the possible protective effects induced by TQ on the histological and biochemical changes that occur in the renal cortex of hypothyroid rats and elucidate the mechanism by which these effects were induced.…”

“…In addition, NS and TQ were recently reported to have the potential to prevent renal tissue damage and fibrosis in lipopolysaccharide- (LPS-) treated rats through its antioxidant and anti-inflammatory effects [ 12 , 13 ]. It was reported that the hydroalcoholic extract of NS could protect the renal tissue against oxidative stress associated with neonatal and juvenile hypothyroidism in rats [ 14 ]…”

Effect of myrtle ( Myrtus communis L.) essential oil on oxidant–antioxidant balance in rats with propylthiouracil‐induced hypothyroidism

“…Thyroid hormones are necessary for carrying out cellular functions in the body. It is also known that these hormones act on oxidative and antioxidative states (Joshi et al., 2018; Mohebbati et al, 2017). OS, which emerged with the change in oxidant and antioxidant balance in favor of oxidants, has an important role in the etiology of many diseases.…”

“…ROS formation as a result of the changes in thyroid hormones has been reported to be conflicting in both HT and hyperthyroidism in many studies. (Aslan et al., 2011; Cano‐Europa et al., 2010; Harper & Seifert, 2008; Mohebbati et al., 2017; Ortiz‐ButrÓn et al, 2011).…”