The antioxidant effect of dexmedetomidine on testicular ischemia-reperfusion injury

Abstract

Turkey. Critical revision, final approval of the manuscript. ABSTRACT PURPOSE:To investigate the protective effect of dexmedetomidine (Dex) on testicular damage induced by ischemia-reperfusion injury in rats. METHODS:Sham group underwent left scrotal exploration only (group 1). The ischemia-reperfusion only group underwent left testicular torsion and detorsion (group 2). The ischemia-reperfusion plus Dex group underwent left testicular torsion, received 50 µg/kg Dex (group 3) and 100 µg/kg Dex (group 4) intraperitoneally at minute 180 of ischemia and then underwent detorsion. We determined histopathological findings and performed specific biochemical analyses. RESULTS:Increasing doses of Dex significantly increased TAS, and significantly decreased OSI. Analyzing the antioxidant effects of increasing doses of Dex in torsion and contrlateral testicles: Dex 100 µg/kg statistically significant increased the tissue total antioxidant status (TAS) and oxidative stress index (OSI) when compared with Dex 50 µg/kg but not found significantly change on the tissue total oxidant status (TOS). However, Dex did not significantly improve these histological alterations. CONCLUSION:The antioxidant effects of dexmedetomidine on testicular ischemia-reperfusion injury in ipsilateral and contrlateral testis, but in the histopathological level, there was no difference statistically according to Johnsen's scoring system between groups at both sides.

Protective Effects of Dexmedetomidine on Ischemia-Reperfusion Injury in Rat Ovary

“…However, when the dexmedetomidine effect is evaluated histopathologically, there is no significant evidence. 12 We found highest TAS level in both Dexmedetomidine group (10 µg and 20 µg), and lowest TAS level in Torsion-Detorsion group. However, the assessment of between the dexmedetomidine groups (10 µg and 20 µg) were observed equally effective in improving the TAS levels.…”

“…20 In studies Dexmedetomidine has been shown to be useful in intestinal, testicular, kidney, lung and cardiac I/R injury. [11][12][13][14][15] There is no study that showing the protective effect of dexmedetomidine on the I/R related tissue damage in rat ovary. We purposed to evaluate whether dexmedetomidine administration can protect rat ovaries from I/R injury, and this protection may be dose dependent.…”

An experimental study on effects of pyrrolidine dithiocarbamate on ischemia-reperfusion injury in testis

“…22 In previous studies, it's been shown that there were some histopathological changes and a decrease in testicular perfusion not only in ipsilateral torted, but also in the contralateral nontorted testis. 23,24 The agents used for prevention of I/R damage on ipsilateral testis also have beneficial effects on contralateral testis. 23,24 These results could have clinical relevance in the clinical scenario of a necessary orchiectomy.…”

“…23,24 The agents used for prevention of I/R damage on ipsilateral testis also have beneficial effects on contralateral testis. 23,24 These results could have clinical relevance in the clinical scenario of a necessary orchiectomy. We didn't investigate the contralateral testis and this can be a pitfall of our study; investigation of both testis can improve the quality and accuracy of future studies.…”

Medical perspective in testicular ischemia-reperfusion injury

“…It has been suggested that sympatholysis may be a property of Dex, due to its mechanism of action via presynaptic α-adrenoceptors. Through this mechanism, Dex may reduce the noradrenaline release induced by ischemia, thus reducing overall oxidant status (23).…”