Intra-peritoneal administration of Ecballium elaterium diminishes postoperative adhesions

Abstract

PURPOSE:To evaluate the effects of Ecballium elaterium (EE), Elaterium officinarum, in postoperative intraperitoneal adhesions in rats. METHODS:Thirty rats were divided into three groups and underwent midline laparotomy under 35 mg/kg ketamine and 5 mg/kg xylazine anaesthesia. In group 1 (n=10), the sham operation group, the abdominal walls were closed without any process. In group 2 (n=10), the control group, the antimesenteric border of the ceacum and the corresponding parietal peritoneum were abraded with dry sterile gauze. In group 3 (n=10), the EE group, 2.5 mg/kg dose of EE was administered as intraperitoneally to the rats after abrasion. All rats were sacrificed on postoperative day 15. Samples were obtained RESULTS:The adhesion score was significantly decreased in the EE group (p=0.001) in comparison with the control group.Microscopically, the EE and sham groups were significantly lower than that of the control group (p<0.001 and p=0.000, respectively).Furthermore, the measurement of tissue levels of hydroxyproline was significantly lower in the sham and EE groups compared to the control group (sham group: 47.6 ± 10.6, EE group: 62.9 ± 9.7, Control group: 84.2 ± 22.1 mg /L/g-tissue). CONCLUSION:The grade and severity of abdominal adhesion could be significantly reduced through administered Ecballium elaterium and therefore be a suitable anti-inflammatory agent for the prevention of postoperative peritoneal adhesion in the future.

Effects of Ecballium elaterium on brain in a rat model of sepsis-associated encephalopathy

“…Rats in the control group underwent sham laparotomy only, while sepsis and treatment groups were subjected to CLP after laparotomy; 2.5 mg/kg Ecballium elaterium extract and 2 cc saline were administered intraperitoneally (treatment group and sepsis group, respectively) as previously reported [10,11] and then incisions were closed by proper suturing of the peritoneum and skin layers. At post-operative 24 h, the animals were sacrificied and the brains were removed.…”

Cordycepin prevents postoperative formation of intra-abdominal adhesions in a rat model: An experimental study

“…Adhesion may cause infertility, formed when oxygen radicals produced by cells break down lipid-containing structures, such as plasma and cell membranes, in the inflammatory response. [10,11,12] Tissue damage is used to assess the severity of inflammation and adhesion. Another parameter used to evaluate adhesion is hydroxyproline (OH-p), a hydroxylated derivative of the amino acid proline, which is abundant in collagen.…”

Effects of Ecballium Elaterium on Proinflammatory Cytokines in a Rat Model of Sepsis

“…The fresh juice itself is used in treatment of sinusitis and jaundice [13,15]. Many studies have reported that EE also has antibacterial, antifungal, immunomodulatory, adhesion preventive effects, and also beneficial effects on sepsis-induced lung injury [8,9,15,16]. The understanding of underlying mech- …”

“…The insoluble materials were removed by vacuum filtration through Whatman paper. The ethanol in the extract was subsequently removed by evaporation and the extract was freeze dried at −80 • C. The extract was weighed and dissolved in sterile isotonic solution at a concentration of 100 mg/mL and stored in the refrigerator at 8 • C until its use for animal experiments [9].…”

“…It is used for the treatment of sinusitis in Anatolian traditional medicine in Turkey. Recent studies revealed its anti-inflammatory, antimicrobial, antitumor, immunomodulatory, and hepatoprotective properties [7][8][9]. Although some anti-inflammatory properties of EE are known, no study revealed that it promotes protection of distant organs such as liver and has effects on small intestine protection during sepsis.…”