Cordycepin prevents postoperative formation of intra-abdominal adhesions in a rat model: An experimental study

Arslan, Serkan; Zeytun, Hikmet; Basuguy, Erol; İbiloğlu, İbrahim; Uygun, İbrahim; Yılmaz, Ahmet; Tan, İlhan; Toprak, Gülten

doi:10.5505/tjtes.2016.48979

Cited by 6 publications

(7 citation statements)

References 32 publications

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“…In this study, erythrocyte and plasma MDA levels were found to be more important in the control group compared to heparin and sham group. It is observed that this result [7,9,10,12,40] is consistent with the notifications of the researchers. The increase in MDA in the control group is caused by cleavage of lipid-containing structures such as plasma and cell membranes of oxygen radicals by the cells involved in the inflammatory response during the adventitious process [28,43].…”

Section: Discussionsupporting

confidence: 91%

“…GSH level was found to be more important in the control group compared to heparin and sham group. It was seen that the results were similar to the findings of [7,9]. GSH plays a central physiological role in protecting against endogenous and exogenous oxidants, toxic substances, DNA damaging agents and carcinogens, and maintaining body haemostasis and thus it clears oxygen radicals in the environment by maintaining intracellular GSH synthesis [20,39].…”

Section: Discussionsupporting

confidence: 71%

“…Arslan et al [7] found that blood MDA levels were higher in the adhesion group than in the control group. In another study, it was observed that in the comparison of the GSH level between the normal group and adhesion group, the decrease in the adhesion group was found to be at a significant level whereas the MDA level was found to be higher and more important in the adhesion group compared to the normal group [40].…”

Section: Discussionmentioning

confidence: 97%

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Surgery-Induced Changes in Erythrocyte and Plasma Lipid Peroxidation, Enzymatic and Non-Enzymatic Antioxidants of Female Rats: Protective Role of Heparin and Pentoxifylline

Şimşek

Durmuş

Yıldız

et al. 2018

Acta Scientiae. Vet.

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Background: Intra abdominal adhesions are a complication that occurs after many abdominal surgical procedures, especially gynecological operations. These complications occur by irritating the peritoneum due to such circumstances as infection or surgical trauma, and are considered a pathological part of the healing process of peritoneal injury. It manifests itself with symptoms such as pain, intestinal or urethral obstruction and abdominal abscesses. Oxidative stress due to adhesions plays an important role on adhesion formation. In addition to many researches done at the point of prevention of adhesion and decreasing stress parameters, in this study, it was planned to determine the effect of Heparin (H) and Pentoxifylline (PTX) on malondialdehyde (MDA) and some antioxidant values.Materials, Methods & Results: This study was performed on rats and thirty-seven female Sprague-Dawley rats were randomized into four groups. The first group was sham (Sh) group (n = 7) and laparotomy was performed and 2 mL of 0.9 % NaCl was applied. For all other rats (n = 30) the small intestine was withdrawn and the uterus was uncovered and the anti mesenteric surfaces of the left uterine horn and left abdominal wall were superficially tilted until slight bleeding was seen. Lesion areas have been covered. Two mL 0.9 % NaCl to control (C) group (n = 10), 500 IU heparin to group H, and 25 mg / kg Pentoxifylline to group PTX (n = 10) have been given and then the abdominal incision was closed. The adhesion score of group Sh was found to be more important than C and PTX groups (P < 0.05). The adhesion score of group C was determined to be more significant than group H (P < 0.05). In group Sh, erythrocyte reduced glutathione (GSH) levels were found to be more significant (P < 0.01) than C, H and PTX groups whereas it was found that group C was more significant than group H (P < 0.01). In group Sh, glutathione peroxidase (GSH-Px) activity was found to be more significant (P < 0.05) than H and PTX groups, while MDA level was found to be lower and more significant (P < 0.01) than group C. In addition, group C was found to be higher and more significant (P < 0.01) than group H. Plasma vitamin E levels in Sh, H and PTX groups were found to be higher and more significant (P < 0.01) than group C. It is also observed that MDA levels in group C are higher and more significant (P < 0.01) than all groups. Nitric oxide (NO) level in Sh group was found to be lower and more significant (P < 0.05) than the other groups whereas it is determined that NO level in H group is higher and more significant (P < 0.05) than group C. Activites of erythrocyte catalase (CAT) and superoxide dismutase (SOD), the levels of plasma vitamin A and β-carotene were not important in all groups.Discussion: Intra abdominal adhesions that occur due to operations performed in the abdominal region still remain as a major problem. This adhesion brings certain problems with itself. Various applications have been made to reduce these problems. In this study, it was observed that inhibition of heparin and pentoxifylline adhesions contributed to the reduction of MDA levels and the reduction of oxidative damage due to the increase in levels of Vitamin E, GSH, GSH-Px and NO levels. In conclusion, it is suggested that heparin and pentoxifylline should be used to prevent adhesions due to abdominal operations and to reduce oxidative damage.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 71%

Section: Discussionmentioning

confidence: 97%

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Surgery-Induced Changes in Erythrocyte and Plasma Lipid Peroxidation, Enzymatic and Non-Enzymatic Antioxidants of Female Rats: Protective Role of Heparin and Pentoxifylline

Şimşek

Durmuş

Yıldız

et al. 2018

Acta Scientiae. Vet.

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“…In addition, ELA may also improve vascular congestion caused by widely used drugs such as fluoxetine. [ 24 ]…”

Section: Discussionmentioning

confidence: 99%

The role of potassium channels on vasorelaxant effects of elabela in rat thoracic aorta

Şahintürk¹,

İşbil²

2022

Turk Gogus Kalp Dama

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Background: This study aims to investigate the roles of potassium channel subtypes in the vasorelaxant effect mechanism of elabela, which is a recently discovered endogenous apelin receptor ligand. Methods: The vascular rings (4-mm) obtained from the thoracic aortas of 20 male Wistar Albino rats were placed into the isolated tissue bath system. The resting tension was set to 1 g. The aortic rings were contracted with 10-5 molar phenylephrine after the equilibration period (90 min). Elabela was applied cumulatively (10-10-10-6 molar) to the aortic rings in the plateau phase. The experimental protocol was repeated in the presence of specific potassium channel subtype inhibitors to determine the role of potassium channels in the vasorelaxant effect mechanism of elabela. Results: Elabela induced a concentration-dependent vasorelaxation (p<0.001). The maximum relaxation level was approximately 51% according to phenylephrineinduced contraction. Vasorelaxant effect level of elabela statistically significantly decreased after removal of the endothelium (p<0.05). Tetraethylammonium (1 milimolar), 4-Aminopyridine (1 milimolar), glyburide (10 micromolar), and barium chloride (30 micromolar) statistically significantly decreased the vasorelaxant effect level of elabela (p<0.001, p<0.001, p<0.01, and p<0.05 respectively). However, anandamide (10 micromolar) and apamin (100 nanomolar) did not statistically significantly change the vasorelaxant effect level of elabela. Conclusion: Our results suggest that large-conductance calciumactivated, voltage-gated, adenosine triphosphate-sensitive, and inward-rectifier potassium channels are involved in the vasorelaxant effect mechanism of elabela in the rat thoracic aorta.

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“…Despite advances in surgical techniques, adhesion development after abdominal surgery remains a problem [ 1 – 4 ]. Peritoneal damage during surgery, damaged serosal surfaces, tissue ischemia, the presence of foreign materials in the environment, increased vascular permeability on the background, fibroblastic activity with underlying decreased fibrinolytic activity with growth factors, and inflammatory cytokines can cause intra-abdominal adhesions [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

The effect of natural surfactants on the development of postoperative intraabdominal adhesion

GÜLER,

KARAKAYA,

DOĞAN

et al. 2023

Turkish Journal of Medical Sciences

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Background/aim The development of postoperative adhesion after abdominal surgery is sometimes a severe problem. Our study investigates the effectiveness of exogenous surfactant application in preventing adhesion development in the experimental adhesion model. Materials and methods This randomized-controlled interventional study was carried out in the animal laboratory of Kahramanmaraş Sütçü İmam University between March 1 and March 31, 2020. An experimental intra-abdominal adhesion model was established in 24 adult female rats by cecal abrasion. Rats were randomly divided into four groups. Groups I, II, and III were taken intraperitoneally as beractant, poractant, and calfactant applied groups, respectively. Group IV was the control group. Relaparotomy was performed in all groups on the 15th postoperative day, and intra-abdominal adhesions were scored macroscopically according to the Canbaz scoring system. In addition, the cecal regions were evaluated microscopically and scored according to the Zühlke microscopic classification system. The scores of the groups were compared statistically. Results The Zühlke adhesion development score was significantly lower in the exogenous surfactant applied groups. In addition, when the surfactant-applied groups were compared among themselves, it was seen that the adhesion score in the beractant group was significantly better than the other surfactant types (p < 0.01). Conclusion Our study results showed that prophylactic intraperitoneal surfactant application significantly reduced postoperative adhesion development, particularly beractant.

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Cordycepin prevents postoperative formation of intra-abdominal adhesions in a rat model: An experimental study

Abstract: Results indicated that cordycepin effectively reduced adhesion in a rat abrasion model. Thus, this agent may be valuable to prevent postoperative adhesion.

Cited by 6 publications

References 32 publications

Surgery-Induced Changes in Erythrocyte and Plasma Lipid Peroxidation, Enzymatic and Non-Enzymatic Antioxidants of Female Rats: Protective Role of Heparin and Pentoxifylline

Surgery-Induced Changes in Erythrocyte and Plasma Lipid Peroxidation, Enzymatic and Non-Enzymatic Antioxidants of Female Rats: Protective Role of Heparin and Pentoxifylline

The role of potassium channels on vasorelaxant effects of elabela in rat thoracic aorta

The effect of natural surfactants on the development of postoperative intraabdominal adhesion

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