Effect of allopurinol on the kidney function, histology and injury biomarker (NGAL, IL 18) levels in uninephrectomised rats subjected to ischaemia-reperfusion injury

Bussmann, André Roberto; Filho, Marcos Antonio Marton; Módolo, Marília Pinheiro; Módolo, R.P.; Amado, Patrícia; Domingues, Maria Aparecida Custódio; Castiglia, Yara Marcondes Machado; Módolo, Norma Sueli Pinheiro

doi:10.1590/s0102-86502014000800006

Cited by 9 publications

(5 citation statements)

References 20 publications

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“…Moreover, renal I/R resulted in a significant increase in FE Na+ on the 2 time points of follow-up after ischaemia, suggesting a significant impairment of tubular function. These findings confirm that I/R injury to the kidney causes both glomerular and tubular dysfunctions, and are in agreement with those reported by others (Bussmann et al, 2014;Castro et al, 2014;Malek and Nematbakhsh, 2015;Punuru et al, 2014). Renal I/R injury is characterized by decreases in the glomerular filtration rate (GFR), tubular and glomerular damage, impairment in hemodynamic regulation and energy depletion (Malek and Nematbakhsh, 2015).…”

Section: Discussionsupporting

confidence: 91%

Effects of exercise and stevia on renal ischemia/reperfusion injury in rats [pdf]

Elsaid¹,

Khalil²,

Ibrahim³

et al. 2019

Acta Sci Pol Technol Aliment

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Background. The present work was designed to study the effects of methanolic stevia extracts and aerobic exercise and combination of both on renal I/R injury in male rats. Methods. 60 adult male Sprague-Dawley rats were subdivided into five equal groups as sham, control, exercise, stevia, and stevia plus exercise group. After 5 weeks of exercise and stevia, animals were exposed to 45 min of left renal ischemia and right nephrectomy followed by reperfusion. Serum creatinine, creatinine clearance, fractional Na excretion (FE Na +), malondialdehyde (MDA), reduced glutathione (GSH) and catalase (CAT) levels in kidney tissues were measured. Also, renal histopathology and the expression of caspase-3 by immunohistochemical examination were done. Results. The results showed that stevia, exercise or combination of stevia and exercise caused a significant decrease in serum level of creatinine (p < 0.001) and FE Na + (p < 0.001) and an increase in creatinine clearance (p < 0.001). Moreover, this caused a significant decrease in (MDA; p < 0.046) and an increase in GSH (p < 0.01) and CAT (p < 0.01), as well as causing a significant decrease in caspase 3 expression compared to the control group. Conclusion. Pretreatment with either stevia or exercise of combination of both seem to have protective effects on renal I/R injury. However, the protective effect of exercise against renal I/R injury seems to be less than stevia. These effects might be due to attenuation of oxidative stress and apoptosis in kidney tissues.

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Section: Discussionsupporting

confidence: 91%

Effects of exercise and stevia on renal ischemia/reperfusion injury in rats [pdf]

Elsaid¹,

Khalil²,

Ibrahim³

et al. 2019

Acta Sci Pol Technol Aliment

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“…Bussmann et al 7 report that the duration of ischemia directly influences the levels of plasma creatinine and degree of renal injury: plasma creatinine levels increased twice after 25 minutes of ischemia and seven to eight times after 45 minutes, which is associated with significant necrosis. This information was important in this study to induce ischemia for a shorter period, ten minutes, in order to minimize injuries.…”

Section: Discussionmentioning

confidence: 99%

Renal biomarkers of male and female Wistar rats (Rattus norvegicus) undergoing renal ischemia and reperfusion

Bazzano¹,

Restel²,

Porfírio³

et al. 2015

Acta Cir. Bras.

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PURPOSE:To investigate biomarkers of acute renal injury in Wistar rats, subjected to left renal ischemia for 10 minutes, and then compare reperfusion at 24 hours, and at 5, 7, 14 and 21 days after the procedure. METHODS:Eight female and male rats between 60 and 81 days old were used in the Central Animal Facility of the UFMS. Assessed biomarkers included urine protein, urea, creatinine, glucose, sodium, potassium, urine alkaline phosphatase and gamma-glutamyl transferase activities, and protein-to-creatinine ratio; and in serum: urea, creatinine, sodium and potassium, fractional excretion of sodium, potassium, urine flow and creatinine clearance. RESULTS:Greater variance was observed in the parameters at 24 hours and at five days (p<0.05) after reperfusion. On the 21 st day, these parameters approximated those obtained for the control group. CONCLUSIONS:Renal ischemia for 10 minutes was sufficient to raise urine levels of protein, glucose, fractional excretion of potassium, urea, creatinine clearance, urine activity of gamma-glutamyltransferase and alkaline phosphatase enzymes in the first 24 hours, up to five days after reperfusion, which may indicate risk of acute kidney injury, according to the RIFLE classification.

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“…2,3 Prior administration of allopurinol (Alo) has a well-documented protective effect in the first 24 h in animal models of sepsis, [4][5][6][7][8][9][10][11] renal I/R, 3 liver, 12,13 intestinal I/R, 14 tourniquets on rat hind-limbs, 15 brain I/R, 16 cocaine-induced diastolic dysfunction, 17 hyperthermia, 18 fructose-fed rats, 19,20 streptozotocin-induced diabetes, 21 and Crotalus terrificus snake venom. 22 However, Alo did not protect against experimental sepsis, [23][24][25] renal I/R, 26 lung I/R, 27 zymosan, 28 or cell culture with LPS, 29 or in animals with infected burns that showed improvement only on day 1, 30 as the protection disappeared subsequently. Alo administration for 14 days in patients treated for burns resulted in lower mortality, 31 but did not protect perioperatively against I/R injury in humans undergoing aortic aneurysm surgery 32 (Supplementary Tables 1 and 2).…”

Section: Introductionmentioning

confidence: 99%

Xanthine oxidase inhibitors and sepsis

Ramos

Barros

Razvickas

et al. 2018

Int J Immunopathol Pharmacol

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Xanthine oxidase activation occurs in sepsis and results in the generation of uric acid (UrAc) and reactive oxygen species (ROS). We aimed to evaluate the effect of xanthine oxidase inhibitors (XOis) in rats stimulated with lipopolysaccharide (LPS). LPS (10 mg/kg) was administered intraperitoneally (i.p.) immediately after allopurinol (Alo, 2 mg/kg) or febuxostat (Feb, 1 mg/kg) every 24 h for 3 days. To increase UrAc levels, oxonic acid (Oxo) was administered by gavage (750 mg/kg per day) for 5 days. Animals were divided into the following 10 groups (n = 6 each): (1) Control, (2) Alo, (3) Feb, (4) LPS, (5) LPSAlo, (6) LPSFeb, (7) Oxo, (8) OxoLPS, (9) OxoLPSAlo, and (10) OxoLPSFeb. Feb with or without Oxo did not aggravate sepsis. LPS administration (with or without Oxo) significantly decreased the creatinine clearance (ClCr) in LPSAlo (60%, P < 0.01) versus LPS (44%, P < 0.05) and LPSFeb (35%, P < 0.05). Furthermore, a significant increase in mortality was observed with LPSAlo (28/34, 82%) compared to LPS treatment alone (10/16, 63%) and LPSFeb (11/17, 65%, P < 0.05). In addition, increased levels of thiobarbituric acid reactive substances (TBARS), tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-10 were observed at 72 h compared to the groups that received LPS and LPSFeb with or without Oxo. In this study, coadministration of Alo in LPS-induced experimental sepsis aggravated septic shock, leading to mortality, renal function impairment, and high ROS and proinflammatory IL levels. In contrast, administration of Feb did not potentiate sepsis, probably because it did not interfere with other metabolic events.

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Effect of allopurinol on the kidney function, histology and injury biomarker (NGAL, IL 18) levels in uninephrectomised rats subjected to ischaemia-reperfusion injury

Cited by 9 publications

References 20 publications

Effects of exercise and stevia on renal ischemia/reperfusion injury in rats [pdf]

Effects of exercise and stevia on renal ischemia/reperfusion injury in rats [pdf]

Renal biomarkers of male and female Wistar rats (Rattus norvegicus) undergoing renal ischemia and reperfusion

Xanthine oxidase inhibitors and sepsis

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