Median lethal needle caliber in two models of experimental sepsis

d'Acâmpora, Armando José; Locks, Giovani de Figueiredo

doi:10.1590/s0102-86502014000100001

Cited by 3 publications

(3 citation statements)

References 32 publications

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“…In the present study, we compared the effects 0.9% saline, modified coconut water (Na 3%) and water coconut 3% + Na + simvastatin, and normal coconut water on serum cytokines, biochemical and white blood cell count in rodent model with hemorrhagic shock and abdominal sepsis. Experimental models of sepsis and hemorrhagic shock has often been employed in other estudies [30][31][32] . In the present study, we found that treatment of rats with sepsis and shock with coconut water modified with sodium 3% + simvastatin reduced serum cytokines, biochemical parameters and peripheral leukocytes.…”

Section: Discussionmentioning

confidence: 99%

Effects of cococonut water and simvastatin in the treatment of sepsis and hemorrhagic shock in rats

et al. 2016

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PURPOSE:To evaluate the effects of modified coconut water as fluid of resuscitation combined with simvastatin in hemorrhagic shock and sepsis model in rats. METHODS:Four groups of Wistar rats with hemorrhagic shock and abdominal sepsis were studied (n=8/group). Rats were bled and maintained at a mean blood pressure 35mmHg for 60min. They were then resuscitated with: 1) saline 0.9%; 2) coconut water+3% NaCl;3) coconut water+NaCl 3%+simvastatin microemulsion (10 mg/kg i.v.; 4) normal coconut water. At 8h post-resuscitation, blood and lungs were collected for exams. RESULTS:Clinical scores, TNF-α, IL-1β, liver/kidney proof levels, and lung injury were significantly reduced in coconut water+NaCl 3%+simvastatin group treated rats, comparing with the other resuscitation treatments. CONCLUSIONS:Resuscitation with coconut water with Nacl 3%+simvastatin had a significant beneficial effect on downregulating cytokines and decreasing lung injury in a rat model of abdominal sepsis and hemorrhagic shock. We also demonstrated that coconut water with Nacl 3%+simvastatin administration clearly made liver and kidney function better and improved clinical score.Key words: Shock, Hemorrhagic. Sepsis. Resuscitation. Simvastatin. Foods Containing Coconut. Rats. Effects of cococonut water and simvastatin in the treatment of sepsis and hemorrhagic shock in rats

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Section: Discussionmentioning

confidence: 99%

Effects of cococonut water and simvastatin in the treatment of sepsis and hemorrhagic shock in rats

et al. 2016

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“…The polymicrobial sepsis was induced using the cecal ligation and puncture (CLP) model according to D'Acampora and Locks, with some adaptations ( 9 , 22 ). To summarize, animals were anesthetized with intraperitoneal injection of ketamine (100 mg/kg) and xylazine (10 mg/kg), a small one-centimeter laparotomy performed, and the cecum exposed and then ligated using a 3–0 silk suture.…”

Section: Methodsmentioning

confidence: 99%

Agaricus brasiliensis Mushroom Protects Against Sepsis by Alleviating Oxidative and Inflammatory Response

et al. 2020

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Sepsis is characterized by the host's dysregulated immune response to an infection followed by a potentially fatal organ dysfunction. Although there have been some advances in the treatment of sepsis, mainly focused on broad-spectrum antibiotics, mortality rates remain high, urging for the search of new therapies. Oxidative stress is one of the main features of septic patients, so antioxidants can be a good alternative treatment. Agaricus brasiliensis is a nutraceutical rich in bioactive compounds such as polyphenols and polysaccharides, exhibiting antioxidant, antitumor, and immunomodulatory activities. Here, we investigated the immunomodulatory and antioxidant effects of A. brasilensis aqueous extract in the cecal ligation and puncture (CLP) sepsis model. Our data showed that aqueous extract of A. brasiliensis reduced systemic inflammatory response and improved bacteria clearance and mice survival. In addition, A brasiliensis decreased the oxidative stress markers in serum, peritoneal cavity, heart and liver of septic animals, as well as ROS production ( in vitro and in vivo ) and tert -Butyl hydroperoxide-induced DNA damage in peripheral blood mononuclear cells from healthy donors in vitro . In conclusion, the aqueous extract of A. brasiliensis was able to increase the survival of septic animals by a mechanism involving immunomodulatory and antioxidant protective effects.

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“…doses of saline, ceftriaxone (CEF—20 mg/kg), or salivary gland extract (SGE—3.5 μg/animal) 24 h beforehand and at time zero, and then the CLP was performed ( Figure 1 ). The polymicrobial sepsis was induced using the CLP method according to the experimental protocol described above by d’Acampora and Locks (2014) and Rittirsch et al (2014), following some adaptations [ 20 , 21 ]. Briefly, mice were anaesthetized by intraperitoneal (i.p.)…”

Section: Methodsmentioning

confidence: 99%

Salivary Gland Extract from Aedes aegypti Improves Survival in Murine Polymicrobial Sepsis through Oxidative Mechanisms

Gomes

Navegantes-Lima

Monteiro

et al. 2018

Cells

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Sepsis is a systemic disease with life-threatening potential and is characterized by a dysregulated immune response from the host to an infection. The organic dysfunction in sepsis is associated with the production of inflammatory cascades and oxidative stress. Previous studies showed that Aedes aegypti saliva has anti-inflammatory, immunomodulatory, and antioxidant properties. Considering inflammation and the role of oxidative stress in sepsis, we investigated the effect of pretreatment with salivary gland extract (SGE) from Ae. aegypti in the induction of inflammatory and oxidative processes in a murine cecum ligation and puncture (CLP) model. Here, we evaluated animal survival for 16 days, as well as bacterial load, leukocyte migration, and oxidative parameters. We found that the SGE pretreatment improved the survival of septic mice, reduced bacterial load and neutrophil influx, and increased nitric oxide (NO) production in the peritoneal cavity. With regard to oxidative status, SGE increased antioxidant defenses as measured by Trolox equivalent antioxidant capacity (TEAC) and glutathione (GSH), while reducing levels of the oxidative stress marker malondialdehyde (MDA). Altogether, these data suggest that SGE plays a protective role in septic animals, contributing to oxidative and inflammatory balance during sepsis. Therefore, Ae. aegypti SGE is a potential source for new therapeutic molecule(s) in polymicrobial sepsis, and this effect seems to be mediated by the control of inflammation and oxidative damage.

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Median lethal needle caliber in two models of experimental sepsis

Cited by 3 publications

References 32 publications

Effects of cococonut water and simvastatin in the treatment of sepsis and hemorrhagic shock in rats

Effects of cococonut water and simvastatin in the treatment of sepsis and hemorrhagic shock in rats

Agaricus brasiliensis Mushroom Protects Against Sepsis by Alleviating Oxidative and Inflammatory Response

Salivary Gland Extract from Aedes aegypti Improves Survival in Murine Polymicrobial Sepsis through Oxidative Mechanisms

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