Effect of N-acetylcysteine in liver ischemia-reperfusion injury after 30% hepatectomy in mice

Lee, Edwin Jin Su; Silva, Sonia Maria da; Simões, Manuel de Jesus; Montero, Edna Frasson de Souza

doi:10.1590/s0102-86502012000400011

Cited by 13 publications

(12 citation statements)

References 12 publications

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“…Control groups received PBS only. Twenty-four hours after the last drug administration, 1.5 ml of blood were collected from overnight-fasted control and treated animals under anesthesia by ketamine/xylazine (70 and 10 mg/kg, respectively, in combination) [13] using cardiac puncture. Serum was harvested from blood samples for clinical pathology.…”

Section: Methodsmentioning

confidence: 99%

Exendin-4 protects mice from D-galactose-induced hepatic and pancreatic dysfunction

Ahangarpour

Oroojan

Badavi

2017

Pathobiology of Aging & Age-related Diseases

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Investigations into pharmaceutical intervention of pancreatic and hepatic dysfunction associated with metabolic disturbances have received relatively little attention. The aim of this study was to investigate the protective effects of exendin-4 in mice receiving D-galactose, a reducing sugar that triggers ROS production and inflammatory mediators affecting the pancreas and liver. Exendin-4 is an United States Food and Drug Administration (FDA) approved glucagon-like peptide that increases insulin dependent glycogen synthesis and glucose uptake. Male NMRI mice (20–25 g), 3 months of age, were randomly divided into 6 groups of 12 mice each: control, exendin-4 (1 nmol/kg), exendin-4 (10 nmol/kg), D-galactose, D-galactose + exendin-4 (1 nmol/kg) and D-galactose + exendin-4 (10 nmol/kg). D-galactose (500 mg/kg) was given daily by oral gavage for 6 weeks. During the last 10 days, exendin-4 (1 and 10 nmol/kg) was injected intraperitoneally daily. Glucose, insulin, insulin resistance, lipid profiles, and hepatic enzyme levels significantly increased in the D-galactose group (p < 0.05), along with a significant decrease in superoxide dismutase activity and pancreatic islet insulin secretion (p < 0.05). Exendin-4 decreased D-galactose-induced increases in serum glucose and insulin, insulin resistance, lipid profiles, and hepatic enzymes, and improved pancreatic islet insulin secretion and antioxidant defense status. The results show that exendin-4 can prevent complications in mice with compromised pancreatic and hepatic function. Long term administration of D-galactose in mice may be a useful model to study insulin resistance, metabolic syndrome, and aging.

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Section: Methodsmentioning

confidence: 99%

Exendin-4 protects mice from D-galactose-induced hepatic and pancreatic dysfunction

Ahangarpour

Oroojan

Badavi

2017

Pathobiology of Aging & Age-related Diseases

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“…Blood samples were centrifuged at 3000 rpm for 15 min to harvest their serum contents. Then, all samples maintained at −20 °C until hormonal assessment(13). Glucose was measured by biochemical assay kits (Pars Azmoon, Iran) and serum insulin levels were determined by ELISA assays kits (Monobind, USA) (The sensitivity of hormone detection per assay tube was 0.182 μIU/mL).…”

Section: Methodsmentioning

confidence: 99%

Pancreatic protective and hypoglycemic effects of Vitex agnus-castus L. fruit hydroalcoholic extract in D-galactose-induced aging mouse model

et al. 2017

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D-galactose induces pancreatic disorder along with aging mouse model. Vitex agnus-castus (VAC) has potential pancreatic protective effect. Hence, this study was designed to evaluate the hypoglycemic and pancreas protective effects of VAC hydroalcoholic extract in D-galactose-induced aging female mice. In the present experimental study, 72 adult female Naval Medical Research Institute (NMRI) mice (weighing 30–35 g) were divided into 6 groups of control, VAC hydroalcoholic extract, D-galactose, D-galactose + VAC hydroalcoholic extract, aged, aged + VAC hydroalcoholic extract. The aged model was prepared by subcutaneous injection of D-galactose for 45 days and, VAC hydroalcoholic extract was gavaged twice a day in the last 7 days. 24 h after the last drug and extract administrations, serum samples and pancreatic tissues were removed to evaluate experimental and histological determinations. Serum glucose level decreased in VAC, D-galactose and, aged-treated groups compared to the control (P < 0.05). Insulin level increased in VAC and decreased in D-galactose and aged VAC-treated mice compared to the control (P < 0.05). Homeostasis model assessment-estimated insulin resistance (HOMA-IR) increased in D-galactose, aging, and VAC hydroalcoholic extract groups (P < 0.05) and, administration of VAC hydroalcoholic extract improved HOMA-IR in D-galactose and aging treated animals. Despite the size of pancreatic islets decreased in aged and D-galactose groups, VAC administration recovered it. Present data showed that VAC hydroalcoholic extract has hypoglycemic and pancreatic protective effects in natural aged and aging model mice.

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“…The mainstay of treatment is supportive with blood products administered to support synthetic function, aggressive investigation and treatment of infection, and radiological investigation to ensure patency of major vascular and biliary structures. The use of exogenous antioxidants such as N-acetylcysteine (Parvolex ® ) has been used by some including our own unit in attempting to reduce the damage by oxygen free radical associated ischaemic reperfusion injury of the liver [82] . However this remains to be accepted as universal practice and currently lacks a strong evidence base [83,84] .…”

Section: Bile Leaksmentioning

confidence: 99%

Operative terminology and post-operative management approaches applied to hepatic surgery: Trainee perspectives

Farid

Prasad²,

Morris‐Stiff³

2013

WJGS

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Outcomes in hepatic resectional surgery (HRS) have improved as a result of advances in the understanding of hepatic anatomy, improved surgical techniques, and enhanced peri-operative management. Patients are generally cared for in specialist higher-level ward settings with multidisciplinary input during the initial post-operative period, however, greater acceptance and understanding of HRS has meant that care is transferred, usually after 24-48 h, to a standard ward environment. Surgical trainees will be presented with such patients either electively as part of a hepatobiliary firm or whilst covering the service on-call, and it is therefore important to acknowledge the key points in managing HRS patients. Understanding the applied anatomy of the liver is the key to determining the extent of resection to be undertaken. Increasingly, enhanced patient pathways exist in the post-operative setting requiring focus on the delivery of high quality analgesia, careful fluid balance, nutrition and thromboprophlaxis. Complications can occur including liver, renal and respiratory failure, hemorrhage, and sepsis, all of which require prompt recognition and management. We provide an overview of the relevant terminology applied to hepatic surgery, an approach to the post-operative management, and an aid to developing an awareness of complications so as to facilitate better confidence in this complex subgroup of general surgical patients. Core tip: Applied anatomy as used in hepatic surgery is different to the traditional morphological teaching. Applied hepatic anatomy is complex but trainees require an understanding of the basic principles to allow an appreciation of the operations performed. Complications require a low threshold of suspicion as they often have important consequences in relation to patient outcome. Recognition of such with rapid alerting of senior staff can facilitate timely and effective management. To date, no universal protocol exists for management of the post-operative period and varies from centre to centre. We provide a practical overview of the terminology, post-operative management, and complications associated with hepatic surgery.

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Effect of N-acetylcysteine in liver ischemia-reperfusion injury after 30% hepatectomy in mice

Cited by 13 publications

References 12 publications

Exendin-4 protects mice from D-galactose-induced hepatic and pancreatic dysfunction

Exendin-4 protects mice from D-galactose-induced hepatic and pancreatic dysfunction

Pancreatic protective and hypoglycemic effects of Vitex agnus-castus L. fruit hydroalcoholic extract in D-galactose-induced aging mouse model

Operative terminology and post-operative management approaches applied to hepatic surgery: Trainee perspectives

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