Exendin-4 protects mice from D-galactose-induced hepatic and pancreatic dysfunction

Ahangarpour, Akram; Oroojan, Ali Akbar; Badavi, Mohammad

doi:10.1080/20010001.2017.1418593

Cited by 13 publications

(18 citation statements)

References 22 publications

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“…Our results revealed significant decrease in insulin expression in islets of Langerhans in the D-gal group, which agrees with necrosis of islets of Langerhans as revealed in the histopathological examination. Our results are in agreement with a report by Ahangarpour et al [ 31 ], who noticed considerable decrease in pancreatic islet insulin secretion in the D-gal group in mice. On the other hand, D-gal increased serum insulin levels and insulin resistance in the brain, which leads to dementia [ 32 ].…”

Section: Discussionsupporting

confidence: 94%

Quercetin Attenuates Pancreatic and Renal D-Galactose-Induced Aging-Related Oxidative Alterations in Rats

El‐Far

Lebda

Noreldin

et al. 2020

IJMS

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Aging is an oxidative stress-associated process that progresses with age. Our aim is to delay or attenuate these oxidative alterations and to keep individuals healthy as they age using natural compounds supplementation. Therefore, we conducted the present study to investigate the protective potentials of quercetin against D-galactose (D-gal)-associated oxidative alterations that were induced experimentally in male Wistar rats. Forty-five rats were randomly allocated into five groups of nine rats each. The groups were a control group that was reared on a basal diet and injected subcutaneously with 120 mg D-gal dissolved in physiological saline solution (0.9% NaCl) per kg body weight daily and quercetin-treated groups that received the same basal diet and subcutaneous daily D-gal injections were supplemented orally with 25, 50, and 100 mg of quercetin per kg body weight for 42 days. Pancreatic and renal samples were subjected to histopathological, immunohistochemical, and relative mRNA expression assessments. Aging (p53, p21, IL-6, and IL-8), apoptotic (Bax, CASP-3, and caspase-3 protein), proliferative (Ki67 protein), antiapoptotic (Bcl2 and Bcl2 protein), inflammatory (NF-κB, IL-1β, and TNF-α), antioxidant (SOD1), and functional markers (GCLC and GCLM genes and insulin, glucagon, and podocin proteins) were determined to evaluate the oxidative alterations induced by D-gal and the protective role of quercetin. D-gal caused oxidative alterations of the pancreas and kidneys observed via upregulations of aging, apoptotic, and inflammatory markers and downregulated the antiapoptotic, proliferative, antioxidant, and functional markers. Quercetin potentially attenuated these aging-related oxidative alterations in a dose-dependent manner. Finally, we can conclude that quercetin supplementation is considered as a promising natural protective compound that could be used to delay the aging process and to maintain human health.

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Section: Discussionsupporting

confidence: 94%

Quercetin Attenuates Pancreatic and Renal D-Galactose-Induced Aging-Related Oxidative Alterations in Rats

El‐Far

Lebda

Noreldin

et al. 2020

IJMS

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“…Homeostasis model assessment‐estimated insulin resistance (HOMA‐IR) was calculated according to the formula: fasting insulin (uIU dL −1 ) × fasting glucose (mg dL −1 )/405. [ 35 ]…”

Section: Methodsmentioning

confidence: 99%

Effects of High Intakes of Fructose and Galactose, with or without Added Fructooligosaccharides, on Metabolic Factors, Inflammation, and Gut Integrity in a Rat Model

Omar

Frank

Kruger

et al. 2021

Molecular Nutrition Food Res

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Scope A high fructose and galactose intake show adverse metabolic effects in animal models and in humans, but it is yet unknown if addition of fermentable dietary fiber can mitigate such effects. This study investigate the effects of high intakes of fructose and galactose, with/without added fructooligosaccharides (FOS), on metabolic factors, inflammation, and gut integrity markers in rats. Methods and Results Rats (n = 6/group) receive different carbohydrates at isocaloric conditions for 12 weeks as follows: 1) starch (control), 2) fructose, 3) galactose, 4) starch + FOS (FOS control), 5) fructose + FOS, and 6) galactose + FOS, together with a high amount of n‐6 polyunsaturated fatty acids (n‐6 PUFA) in all diets except for in 7) starch + olive oil (negative control). The rats fed the galactose and galactose + FOS diets exhibit lower body weight than other groups. High‐galactose diets has more pronounced effects on metabolic factors and gut permeability than high‐fructose diets. High‐fructose diets show less pronounced effect on these selected markers. No differences in inflammatory markers are detected for any of the diets. Conclusions The results suggest potential adverse effects of high galactose and fructose on metabolic factors and gut integrity markers, but not on inflammation. However, several mechanisms are at play, and general net effects are difficult to determine conclusively for the conditions tested.

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“…The pancreatic Langerhans islets are transferred to 2 mL microtubes containing Krebs-bicarbonate buffer in addition to basal 2.8, moderate 6.5, and excitation 16.7 mM concentrations of glucose (Merck, Germany) [ 8 ]. Then, 50, 100, and 200 µ M of eugenol (Kemdent, United Kingdom) was added to the microtubes and incubated at 37ºC for 60 min [ 9 ].…”

Section: Methodsmentioning

confidence: 99%

Eugenol Improves Insulin Secretion and Content of Pancreatic Islets from Male Mouse

Oroojan

2020

International Journal of Endocrinology

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Glucose homeostasis is required for control of insulin secretion. Phenolic compounds improved glucose-stimulated insulin secretion (GSIS). Eugenol is a phenolic compound that may increase GSIS. So, it was decided to investigate the effect of eugenol on the insulin secretion and content of pancreatic islets from the male mice. In this experimental study, 3-month-old NMRI mice (20–25 g) were prepared. The pancreatic islets of Langerhans were isolated using the collagenase digestion method and divided into 12 groups: glucose 2.8, 5.6, and 16.7 mM, glucose 2.8 mM + eugenol 50, 100, and 200 µM, glucose 5.6 mM + eugenol 50, 100, and 200 µM, and glucose 16.7 mM + eugenol 50, 100, and 200 µM. The islet’s insulin secretion and content were measured after 1 hour and 24 hours incubation at 37°C, respectively, by the ELISA assays method and related commercial kit. Present results showed that all doses of eugenol increased islet’s insulin secretion and content in the medium containing glucose concentrations 2.8, 5.6, and 16.7 mM (P < 0.05). In conclusion, eugenol as a phenolic compound increased insulin secretion and content of pancreatic islets. The moderate dose of this compound enhanced insulin secretion during hypo- and hyperglycemic conditions, as well as a high dose of eugenol, increased insulin content. Finally, present research suggested that the administration of eugenol 100 µM was suitable for the early stage of T2DM as well as eugenol 200 µM for the advanced stage of this disease.

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Exendin-4 protects mice from D-galactose-induced hepatic and pancreatic dysfunction

Cited by 13 publications

References 22 publications

Quercetin Attenuates Pancreatic and Renal D-Galactose-Induced Aging-Related Oxidative Alterations in Rats

Quercetin Attenuates Pancreatic and Renal D-Galactose-Induced Aging-Related Oxidative Alterations in Rats

Effects of High Intakes of Fructose and Galactose, with or without Added Fructooligosaccharides, on Metabolic Factors, Inflammation, and Gut Integrity in a Rat Model

Eugenol Improves Insulin Secretion and Content of Pancreatic Islets from Male Mouse

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