Effect of a hyperlipidic diet rich in omegas 3, 6 and 9 on aberrant crypt formation in rat colonic mucosa

Burlamaqui, Idália Maria Brasil; Dornelas, Conceição Aparecida; Júnior, José Telmo Valença; Mota, Daniel Magalhães Coutinho; Mesquita, Francisco José Cabral; Veras, Lara Burlamaqui; Vasconcelos, Paulo Roberto Leitão de; Rodrigues, Lusmar Veras

doi:10.1590/s0102-86502012000100006

Cited by 7 publications

(4 citation statements)

References 34 publications

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“…Also, the emergence of spontaneous ACF has been described in Fischer 344 rats without the use of a carcinogen 27 . Recently, Burlamaqui et al 28 , using azoxymethane for experimental colon carcinogenesis, reported the presence of ACF, albeit scarce, in rats that received no carcinogen.…”

Section: Discussionmentioning

confidence: 99%

Carcinogenesis in rats subjected to a new model ureterosigmoidostomy and treated with L-lysine

Santos¹,

Coelho

Azevedo

et al. 2016

Acta Cir. Bras.

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PURPOSE:To evaluate the effects of L-lysine on the intestinal and urothelial epithelium of rats subjected to ureterosigmoidostomy (new model for surgical carcinogenesis). METHODS:Forty-two rats, 9 weeks of age, were divided into 6 groups. Animals in groups A, B, C were subjected to ureterosigmoidostomy (US) and treated with L-lysine, celecoxib and H 2 O, respectively. Groups D, E and F (non-operated controls) received L-lysine, celecoxib and H 2 O, respectively. The L-lysine dose was 150 mg/kg and that of celecoxib was 20 mg/kg. The colon was analyzed for the presence of aberrant crypt foci (ACF) under a stereomicroscope.The tissue was stained with hematoxylin and eosin and PAS alcian blue. RESULTS:There were rare ACF, and there was no statistically significant difference between the groups. Histopathologic study of the ureteral epithelium identified moderate to severe urothelial hyperplasia in rats with ureterosigmoidostomy. Transitional hyperplasia in the ureters of animals receiving L-lysine (A) showed an apparent difference compared to the control (C) (P=0.2424). There was no dysplasia or atypia.CONCLUSION: L-lysine does not promote carcinogenesis of the intestinal and urethelial epithelium of rats subjected to ureterosigmoidostomy at the doses and times studied.

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Section: Discussionmentioning

confidence: 99%

Carcinogenesis in rats subjected to a new model ureterosigmoidostomy and treated with L-lysine

Santos¹,

Coelho

Azevedo

et al. 2016

Acta Cir. Bras.

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“…The dysplastic ACF may present microsatellite instability, methylation with epigenetic silencing 14 . For this reason, it has been consecrated as a model for trials of new anticancer molecules, using the model in rats subjected to chemical carcinogenesis 13,15 . Do urinary derivations follow the course of this aberrant crypt foci model?…”

Section: Discussionmentioning

confidence: 99%

Bladder carcinogenesis in rats subjected to ureterosigmoidostomy and treated with L-lysine

Dornelas

Santos

Correia

et al. 2016

Rev. Col. Bras. Cir.

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Objective: to evaluate the effect of L-lysine in the bladder and intestinal epithelia in rats submitted to vesicosigmoidostomy. Methods: we divided forty Wistar rats into four groups: group I -control group (Sham); group II -submitted to vesicosigmoidostomy and treated with L-lysine 150mg/kg; group III -submitted only to vesicosigmoidostomy; and group IV -received L-lysine 150mg/kg. After eight weeks the animals were sacrificed. Results: in the bladders of all operated animals we observed simple, papillary and nodular hyperplasia of transitional cells, transitional cell papillomas and squamous metaplasia. As for the occurrence of aberrant crypt foci in the colons of operated animals, we did not observe statistically significant differences in any of the distal, proximal and medium fragments, or in all fragments together (p=1.0000). Conclusion: Although statistically there was no promotion of carcinogenesis in the epithelia of rats treated with L-lysine in the observed time, it was clear the histogenesis of bladder carcinogenesis in its initial phase in all operated rats, this being probably associated with chronic infection and tiny bladder stones.

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“…The Wistar rat can be used in the study of colorectal carcinogenesis, but it requires more prolonged exposure to the carcinogen 19 . Transgenic mice or rats are also widely used to study colon tumorigenesis.…”

Section: Discussionmentioning

confidence: 99%

The role of TNF-α and NFkβ in an experimental model of intestinal carcinogenesis with 1,2-dimethyhydrazine

Barreira,

Campelo,

Rebouças

et al. 2023

Acta Cir. Bras.

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Purpose: To analyze the potential of tumor necrosis factor-α (TNF-α) and factor nuclear kappa B (NF-κB) as colorectal cancer (CRC) biomarkers in an experimental model of intestinal carcinogenesis with 1,2-dimethyhydrazine (1,2-DMH). Methods: Twenty-four male Wistar rats were divided into two groups: sham and 1,2-DMH. First, 1,2-DMH (20 mg/kg/week) was administered for 15 consecutive weeks. In the 25th week, proctocolectomy was conducted. Histopathological analysis, immunohistochemistry, and gene expression of TNF-α and NF-κB were performed. Statistical analysis was performed using GraphPad Prism. The location of aberrant crypt foci (ACF) was analyzed by Kruskal-Wallis’ test. For analyses with two groups with parametric data, the t -test was used; for non-parametric data, the Mann-Whitney’s test was used. P < 0.05 was considered significant. Results: The number of ACF and macroscopic lesions was significantly higher (p < 0.5) in the 1,2-DMH group compared to the sham group, and most ACF were concentrated in the distal segment of the colon. There was a statistically significant increase (p < 0.5) in protein and gene expression of TNF-α and NF-κB in the 1,2-DMH group compared to the sham group. Conclusions: Our results provide supportive evidence that TNF-α and NF-κB pathways are strongly involved in CRC development in rats and might be used as early biomarkers of CRC pathogenesis in experimental studies.

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Effect of a hyperlipidic diet rich in omegas 3, 6 and 9 on aberrant crypt formation in rat colonic mucosa

Cited by 7 publications

References 34 publications

Carcinogenesis in rats subjected to a new model ureterosigmoidostomy and treated with L-lysine

Carcinogenesis in rats subjected to a new model ureterosigmoidostomy and treated with L-lysine

Bladder carcinogenesis in rats subjected to ureterosigmoidostomy and treated with L-lysine

The role of TNF-α and NFkβ in an experimental model of intestinal carcinogenesis with 1,2-dimethyhydrazine

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