Effect of short-term ornithine alpha-ketoglutarate pretreatment on intestinal ischemia-reperfusion in rats

Gonçalves, Eduardo Silvio Gouveia; Rabelo, Camila Menezes; Neto, Alberico Ximenes do Prado; Garcia, José Huygens Parente; Guimarães, Sérgio Botelho; Vasconcelos, Paulo Roberto Leitão de

doi:10.1590/s0102-86502011000700002

Cited by 6 publications

(3 citation statements)

References 22 publications

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“…It can synthesize glutamine, arginine, proline, and polyamines in vivo [31]. Accumulating evidence has shown that OKG improves nutritional conditions under unhealthy conditions due to its regulatory effect on oxidative stress, tissue injury, and metabolism [12,32,33]. OKG is also more efficient than ornithine and α-ketoglutarate alone [34,35] owing to its extensive hydrogen bond network and electrostatic charges [30].…”

Section: Discussionmentioning

confidence: 99%

“…OKG is an amino acid complex salt formed by binding ornithine and α-ketoglutaric acid with an ionic bond. It provides multiple physiological benefits, including promoting growth [8], antioxidant activity [9,10], anticancer effects [11], anti-inflammatory properties, and decreasing tissue damage [12]. Additionally, amino acid complex salts not only possess the functions of two amino acids but also mutually enhance their physiological activities in the body [13].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Ornithine α-Ketoglutarate on Intestinal Microbiota and Serum Inflammatory Cytokines in Dextran Sulfate Sodium Induced Colitis

Wang

Tian

et al. 2023

Nutrients

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Ornithine α-ketoglutarate (OKG), a nutritional compound, is an amino acid salt with anti-oxidative and anti-inflammatory effects on humans and animals. Ulcerative colitis (UC), as an inflammatory bowel disease (IBD), leads to chronic intestinal inflammatory dysfunction. This study evaluated the optimal dosage of OKG in healthy mice. Then, a mouse model of acute colitis was established using dextran sodium sulfate (DSS), and the preventive effect of OKG on DSS-induced colitis in mice was explored through analysis of serum inflammatory cytokines and fecal microbiota. Initially, the mice were randomly divided into a control group, a group given a low dose of OKG (LOKG: 0.5%), a group given a medium dose of OKG (MOKG: 1%), and a group given a high dose of OKG (HOKG: 1.5%); they remained in these groups for the entire 14-day experimental period. Our results demonstrated that 1% OKG supplementation increased body weight, serum growth hormone (GH), insulin (INS), alkaline phosphatase (ALP), Tyr, and His and decreased urea nitrogen (BUN), NH3L, and Ile. Then, a 2 × 2 factor design was used for a total of 40 mice, with diet (a standard diet or a 1% OKG diet) and challenge (4% DSS or not) as the main factors. During days 14 to 21, the DSS mice were administered 4% DSS to induce colitis. The results revealed that OKG alleviated weight loss and reversed the increases in colonic histological damage induced by DSS. OKG also increased serum IL-10 secretion. Moreover, OKG enhanced the abundance of Firmicutes and decreased that of Bacteriodetes at the phylum level and particularly enhanced the abundance of Alistipes and reduced that of Parabacterioides at the genus level. Our results indicated that OKG promotes growth performance and hormone secretion and regulates serum biochemical indicators and amino acid concentrations. Furthermore, 1% OKG supplementation prevents DSS-induced colitis in mice via altering microbial compositions and reducing the secretion of inflammatory cytokines in serum.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of Ornithine α-Ketoglutarate on Intestinal Microbiota and Serum Inflammatory Cytokines in Dextran Sulfate Sodium Induced Colitis

Wang

Tian

et al. 2023

Nutrients

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“…Intestinal ischemia occurs due to decrease or interruption of arterial and/or venous intestinal blood flow (hypoxia), causing damage to the mucosa. This event may occur due to acute or chronic obstruction of arteries and/or veins such as the celiac trunk, cranial mesenteric artery (CMA), the caudal mesenteric artery and/or corresponding vein, responsible for their irrigation (Carden & Granger 2000, Ribeiro & Yoshida 2005, Gonçalves et al 2011.…”

Section: Introductionmentioning

confidence: 99%

Establishment of an experimental model of small intestinal ischemia and reperfusion injuries in New Zealand rabbits

et al. 2018

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The present study aimed to establish a methodology capable to cause intestinal ischemia and reperfusion injuries, to perform clamping of the jejunal segment of the extramural peri-intestinal marginal artery branch. For this, 37, 10-week-old male New Zealand breed rabbits were used. One rabbit was used to establish the anatomic references for the procedure and was not part of the six experimental groups; the rest were allocated into six experimental groups: Sham group, negative control, subjected only to midline celiotomy; group I1H undergoing vascular occlusion for an hour; group I2H submitted to vascular occlusion for two hours; group I1H/R2H undergoing vascular occlusion for one hour followed by two hours of reperfusion; group I2H/R1H undergoing vascular occlusion for two hours, followed by reperfusion for one hour, and group I2H/R5H undergoing vascular occlusion for two hours followed by reperfusion for five hours. The rabbits were evaluated for the macroscopic aspects (color and peristalsis) of the jejunal segment, as well as the histological aspect, checking for presence or absence of mucosal destruction, edema, hemorrhaging, lymphatic vessel dilatation, and the presence of polymorphonuclear cells. It was observed that the macroscopic and histopathological lesions accentuated in larger employed ischemia and reperfusion times. Rabbits subjected to ischemia for two hours followed by reperfusion for five hours (I2H/R5H) made up the experimental group which was easily reproducible and showed moderate intestinal injury, different from the other groups.

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Investigating viability of intestine using spectroscopy: a pilot study

Karakaş

Sircan-Küçüksayan

Elpek

et al. 2014

Journal of Surgical Research

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Effect of short-term ornithine alpha-ketoglutarate pretreatment on intestinal ischemia-reperfusion in rats

Cited by 6 publications

References 22 publications

Effect of Ornithine α-Ketoglutarate on Intestinal Microbiota and Serum Inflammatory Cytokines in Dextran Sulfate Sodium Induced Colitis

Effect of Ornithine α-Ketoglutarate on Intestinal Microbiota and Serum Inflammatory Cytokines in Dextran Sulfate Sodium Induced Colitis

Establishment of an experimental model of small intestinal ischemia and reperfusion injuries in New Zealand rabbits

Investigating viability of intestine using spectroscopy: a pilot study

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