Metabolic and hematologic consequences of colectomy associated to hepatectomy in rats

Abstract

PURPOSE:To investigate the influence of partial colectomy associated with hepatectomy on the biodistribution of the 99m Tc-phytate, on metabolic parameters, as well as labeling and morphology of red blood cells. METHODS: Wistar rats were distributed into three groups (each with six), nominated as colectomy, colectomy+hepatectomy and sham. In the 30 th postoperative day all rats were injected with 99mTc-phytate 0.1mL i.v. (radioactivity 0.66 MBq). After 15 minutes, liver sample was harvested and weighed. Percentage radioactivity per gram of tissue (%ATI/g) was determined using an automatic gammacounter. Serum AST, ALT, alkaline phosphatase and red blood cells labeling were determined. RESULTS: The liver %ATI/g and red blood cells labeling were lower in colectomy and colectomy+hepatectomy rats than in sham rats (p <0.05), and no difference was detected comparing the colectomy and colectomy+hepatectomy groups. Red blood cells morphology did not differ among groups. Serum levels of AST, ALT and alkaline fosfatase were significantly higher in colectomy+hepatectomy than in colectomy rats (p<0.001). CONCLUSION: Hepatectomy associated with colectomy lowered the uptake of radiopharmaceutical in liver and in red blood cells in rats, coinciding with changes in liver enzymatic activity. Keywords: Colectomy. Hepatectomy. Metabolism. Biological Availability. Technetium. Rats. RESUMO OBJETIVO:Investigar a influência da colectomia associada à hepatectomia parcial, na biodistribuição do fitato99m TcO 4 , na marcação e morfologia de hemácias e em parâmetros metabólicos. MÉTODOS: Ratos Wistar foram distribuídos em três grupos (seis animais cada), denominados: colectomia, colectomia+hepatectomia e sham. No 30º dia pós-operatório, em todos eles foi feita injeção de 0,1 mL i.v. de fitato-

The ileum positively regulates hepatic regeneration in rats

“…Despite the colon resection was not performed in the present study, we intend to use this model in the near future in this research line. In other studies, impaired liver regeneration after bowel resection has been demonstrated [25][26][27][28] , confirming the findings of the present study.…”

Comparison between electrocautery and fibrin selant after hepatectomy in rats

“…However, in EG, despite the levels of leukocytes being normal, this was not confirmed in the macroscopic analysis, which showed the presence of infection. Carvalho et al 13 , when investigating the Table 4 Table 4 Table 4 Table 4 Table 4 -Microscopic analysis of specimens from animals submitted to hepatectomy with hemostasis promoted by TachoSil ® versus electrocautery in the 14 th postoperative day. hematological and metabolic consequences of colectomy combined with partial hepatectomy in rats, found a significantly greater increase in the levels of AST, ALT and alkaline phosphatase in the colectomy and hepatectomy group than in the colectomy alone group, suggesting that hepatectomy was responsible for such enzyme elevation.…”