Effects of sildenafil on the viability of random skin flaps

Abstract

Purpose:To assess the viability of McFarlane skin flaps in rats with administration of sildenafil. Methods: Twenty Wistar rats were distributed into two groups: Control (dorsal skin flap, subdermal application of saline solution at 0.9%) and Study (dorsal skin flap, subdermal application of sildenafil). Seven days after the surgery, flaps were photographed and graphically rendered. Then, they were analyzed with AutoCAD software. Three biopsies (proximal, medial and distal) of each flap were collected for histological analysis. Results: Macroscopic analysis showed that animals of the study group had greater necrotic areas (p=0.003) in the dorsal skin flaps. Additionally, histological analysis of the distal third of these flaps showed a tendency to less granulated tissue formation in animals treated with sildenafil. Conclusion: Sildenafil subdermally was associated with lower viability of the random skin flap in rats. Key words: Wound Healing. Surgical Flaps. Cyclic GMP. Nitric Oxide. Rats. RESUMOObjetivo: Avaliar a viabilidade de retalhos cutâneos de ratos à McFarlane após a administração de sildenafil. Métodos: Vinte ratos Wistar foram distribuídos em dois grupos: Controle (confecção do retalho cutâneo dorsal, aplicação subdérmica de solução salina a 0,9%) e Estudo (confecção do retalho cutâneo dorsal, aplicação subdérmica de sildenafil). Sete dias após a operação, os retalhos foram fotografados e representados graficamente, para serem analisados com o programa AutoCad. Três biópsias (cranial, média e caudal) foram coletadas de cada retalho, para análise histológica. Resultados: A análise macroscópica evidenciou que os animais do grupo Estudo apresentaram maiores áreas de necrose (p=0,003) nos retalhos cutâneos dorsais. Além disso, a análise histológica dos terços distais dos retalhos mostrou uma tendência à formação de menos tecido de granulação nos animais que receberam o sildenafil. Conclusão: O sildenafil subdérmico esteve associado com uma pior viabilidade tecidual dos retalhos cutâneos dorsais de ratos. Descritores: Cicatrização de Feridas. Retalhos Cirúrgicos. GMP Cíclico. Óxido Nítrico. Ratos.

Prevention of unfavourable effects of cigarette smoke on flap viability using botulinum toxin in random pattern flaps: An experimental study

“…Many agents that enhance or reduce flap viability have been reported in the literature. Investigated agents improving flap viability include agents suppressing free radical production (6,7), nitric oxide (8,9), sildenafil (10,11), vascular endothelial growth factor (12) and vasonatrin peptide (13).…”

Description of a new experimental model skin flap for studying skin viability in rats

“…Some were elevated with the base in the caudal part of the animal's back 6 , which others had the base in the cranial part, at the level of the scapulae 7 .…”

“…In addition, the location of the base described as cranial (at the level of the scapulae) has also not been uniform [6][7][8] .…”

The positive effect of Botulinum toxin type A on the viability of random flap in tobacco exposed in rats

“…For this reason, several strategies were studied to decrease surgical complications [4][5][6] , one of the strategies was to use BontA as a vasodilator [7][8][9] .…”