Dimethylsulfoxide attenuates ischemia-reperfusion injury in rat testis

Abstract

Purpose:To evaluate the protective role of dimethylsulfoxide (DMSO) in a rat model of testis ischemia/reperfusion (I/R). Methods: Twenty-four male Wistar rats were randomized in two equal groups. Control rats (G-1) received saline 2.0 ml intraperitoneally (ip) 21, 9 and 1 h before torsion. Experimental rats (G-2) received ip injections of 3% aqueous solution of DMSO, 0.1ml/10g body weight. Saline was added to complete 2.0ml when necessary. I/R injury was induced in anesthetized rats by torsion of the right testis lasting 3 hours. Testis and blood samples were collected at the end of ischemia (T-0) and 3 hours later (T-3) for assessment of testis malonaldehyde (MDA), reduced glutathione (GSH), and plasma total antioxidant power (TAP). Results: MDA levels decreased significantly in G-2 rats compared with G-1 animals in all time-points. GSH levels increased significantly in T-0 and T-3 time-points in DMSO pretreated rats compared with G-1 rats. GSH levels increased significantly during reperfusion in G-2 rats. TAP was similar in both groups denoting absence of systemic effects in this study. Conclusion: Pretreatment with DMSO reduces testis lipid peroxidation and oxidative stress caused by torsion/detorsion of the testis. Key words: Antioxidants. Testis. Oxidative Stress. Malondialdehyde. Glutathione. Rats. RESUMO Objetivo:Avaliar o papel protetor do dimetilsulfóxido (DMSO) utilizando um modelo de isquemia/reperfusão (I/R) do testículo do rato. Métodos: Vinte e quatro ratos machos Wistar, randomizados em dois grupos iguais e tratados com solução salina 2,0 ml (grupo Controle-G-1) ou solução de DMSO (3%, 0,1ml/10g peso corporal), acrescida de solução salina até completar 2,0 ml (grupo Experimento-G-2) por via intraperitoneal, as 21, 9 e 1 h antes da torção. .A lesão de I/R foi induzida por torção do testículo direito e mantida por 3 horas, em ratos previamente anestesiados. Amostras (sangue e testículo direito) foram coletadas ao término da isquemia (T-0) e após 3 horas de reperfusão (T-3) para determinação das concentrações de malonaldeído (MDA), glutationa reduzida (GSH) e capacidade antioxidante total do plasma (CAT). Resultados: As concentrações de MDA diminuíram significativamente nos ratos do G-2. comparados ao G-1, nos tempos estudados. As concentrações de GSH aumentaram significativamente nos testículos dos ratos do G-2 comparado aos controles no T-0 e no T-3 e durante a reperfusão no G-2. Os valores obtidos na análise do CAT foram semelhantes. Conclusão: O pré-tratamento com DMSO reduz a peroxidação lipídica e o estresse oxidativo decorrentes da torção/destorção do testículo, no rato.

Pharmacological postconditioning with atorvastatin calcium attenuates myocardial ischemia/reperfusion injury in diabetic rats by phosphorylating GSK3β

“…On the basis of its antioxidative and anti-inflammatory properties, numerous prior studies have observed the effects of DMSO in various organs during myocardial ischemia, including the heart (55), brain (56), liver (57), gastrointestinal (58), lung (59) and ovaries (60). The usage and dosage were different, and the results vary from against reperfusion injury (3,55,61), no protect effect (3,5,62) to aggravate damage (63). In the present study, TDZD-8 and atorvastatin calcium were dissolved in 0.8% DMSO for administration to rats, and indexes of cardiac injury were compared between DM IR and DM DMSO groups.…”

Hypoxia‐Induced Deacetylation Is Required for Tetraploid Differentiation in Response to Testicular Ischemia‐Reperfusion (IR) Injury

“…Previous studies have demonstrated a time‐dependent occurrence and pathologic induction of germ cell degeneration in the testicular IR model (Ozkurkcugil et al, 2004). Lipid peroxidation has been implicated as one of the most important inducers of tissue damage in the IR model (Guimaraes et al, 2010). Testicular torsion results in high levels of lipid peroxidation in testis, which is usually associated with cell death by necrosis.…”

Medical perspective in testicular ischemia-reperfusion injury

“…DMSO exerts a protective effect against testes lipid peroxidation injury caused by I/R in rats. The increase in GSH levels during reperfusion in DMSO-treated rats favours the hypothesis that the use of this substance in the pretorsion period may attenuate the damaging effects of tIR injury (29).…”