Prevalence of Helicobacter spp in chronic cholecystitis and correlation with changes on the histological pattern of the gallbladder

Abstract

Prevalence of Helicobacter spp in chronic cholecystitis and correlation with changes on the histological pattern of the gallbladder 1Prevalência do Helicobacter spp na colecistite crônica calculosa e correlação com as alterações histológicas da vesícula biliar ABSTRACT Purpose: Establish the prevalence of Helicobacter spp in chronic cholecystitis and its correlation with the gallbladder's histological findings. Methods: 100 patients were operated for chronic cholecystitis with cholecystolithiasis. In pathological examination of the gallbladder, were evaluated the presence of metaplasia, dysplasia, lymphoid follicles, anaplasia and tumors that might be related to the presence of Helicobacter plus the presence of the bacilli Giemsa by optical microscopy. From the DNA extracted from the gallbladder's bile, PCR was performed by using specific primers for the identification of Helicobacter spp with amplification of the 400bp segment of rRNA gene16S, with positive control DNA from Helicobacter pylori. All the cases negative for isolation of genetic material were excluded. The cases of PCR and Giemsa were used as negative control group. The histological findings were compared to the presence of bacilli and PCR data using a chi-square and Fisher's Exact test (CI = 95.0%, p <0.05). Results: Of 68 patients, 42 (61.8%) were PCR for Helicobacter spp and 19 (27.9%) had Giemsa . There was no correlation between the two findings. The PCR for Helicobacter spp was not correlated to the histological findings. The presence of lymphoid follicles and metaplasia was related to the Giemsa (p = 0.025 and p= 0.039). Conclusion: There is high prevalence of Helicobacter spp in patients with chronic cholecystitis and cholecystolithiasis without be correlated with the histological patterns studied.

Helicobacter Pylori infection of the gallbladder and the risk of chronic cholecystitis and cholelithiasis: A systematic review and meta‐analysis

“…H. pylori is a Gram‐negative and microaerophilic microorganism which represents the main pathogenic agent in the development of chronic gastritis, duodenal ulcer, and has been proved as a risk factor for gastric cancer development . Recently, several studies detected various species of H. pylori in tissue and bile taken from the gallbladder . Kawaguchi et al first detected H. pylori in the gallbladder's mucosa of a patient with calculus cholecystitis in 1996.…”

Histomorphological changes in gall bladder diseases and its association with helicobacter infection

“…Similar finding were observed in other studies. 16,17 Many organisms have been isolated worldwide in bile. The route of infection are ascending and descending infections.…”

The Relationship Between Cholecystitis and Presence of Helicobacter pylori in the Gallbladder

“…On the other hand, the results of other studies have shown that there is not any significant relationship between H. pylori infection and chronic cholecystitis, or stone formation in the gallbladder [24][25][26]. Helaly and colleagues showed that H. pylori is present in almost 40.9% of samples in patients with chronic calcular cholecystitis [23].…”