End-to-side nerve repair using fibrin glue in rats

Silva, Daniel Nunes e; Coelho, Julice Medeiros; Frazílio, Fabrício de Oliveira; Odashiro, Alexandre Nakao; Carvalho, Paulo de Tarso Camillo de; Pontes, Elenir Rose Jardim Cury; Vargas, Alexandre Silva de; Rosseto, Marcelo; Silva, Andréia Brochado Antoniolli da

doi:10.1590/s0102-86502010000200007

Cited by 18 publications

(15 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In 2010 we confirmed the effectiveness of this technique through functional and morphometric analysis. However, as already expected, the regeneration of axons despite being satisfactory did not reach normal levels pointing out the need for more studies in order to enhance axon regeneration with this technique 10 .…”

Section: Many Authors Have Recently Enhanced the Results Of End-mentioning

confidence: 59%

“…However, on the 90 th day, these groups presented walking pattern recovery, indicating reestablishment of the motor function after ETS, proving the repair success 10 . There was no difference between the groups regardless the repair (suture X fibrin glue) and the use of NGF.…”

Section: Discussionmentioning

confidence: 75%

“…Therefore the fibrin glue has been recently studied in ETS with the main purpose of preservation of the donating nerve, keeping it completely untouched after the repair 10 . In 2010 we confirmed the effectiveness of this technique through functional and morphometric analysis.…”

Section: Many Authors Have Recently Enhanced the Results Of End-mentioning

confidence: 99%

See 2 more Smart Citations

Nerve growth factor with fibrin glue in end-to-side nerve repair in rats

Silva

Aydos

et al. 2012

Acta Cir. Bras.

Self Cite

View full text Add to dashboard Cite

PURPOSE:To determine the effects of end-to-side nerve repair performed only with fibrin glue containing nerve growth in rats. METHODS:Seventy two Wistar rats were divided into six equal groups: group A was not submitted to nerve section; group B was submitted to nerve fibular section only. The others groups had the nerve fibular sectioned and then repaired in the lateral surface of an intact tibial nerve, with different procedures: group C: ETS with sutures; group D: ETS with sutures and NGF; group E: ETS with FG only; group F: ETS with FG containing NGF. The motor function was accompanied and the tibial muscle mass, the number and diameter of muscular fibers and regenerated axons were measured. RESULTS:All the analyzed variables did not show any differences among the four operated groups (p>0.05), which were statistically superior to group B (p<0.05), but inferior to group A (p>0.05). CONCLUSION:The end-to-side nerve repair presented the same recovery pattern, independent from the repair used, showing that the addition of nerve growth factor in fibrin glue was not enough for the results potentiating.Key words: Suture Techniques. Fibrin Tissue Adhesive. Microsurgery. Rats. RESUMO OBJETIVO:Determinar os efeitos do reparo nervoso término-lateral realizado apenas com cola de fibrina contendo fator de crescimento nervoso em ratos. MÉTODOS:Setenta e dois ratos Wistar foram distribuídos em seis grupos: A -não submetido à secção nervosa; B -secção do nervo fibular (sem reparo); Os outros grupos tiveram o nervo fibular seccionado e então reparado na superfície lateral do nervo tibial

show abstract

Section: Many Authors Have Recently Enhanced the Results Of End-mentioning

confidence: 59%

Section: Discussionmentioning

confidence: 75%

See 1 more Smart Citation

Nerve growth factor with fibrin glue in end-to-side nerve repair in rats

Silva

Aydos

et al. 2012

Acta Cir. Bras.

Self Cite

View full text Add to dashboard Cite

show abstract

“…The end-to-side neurorrhaphy without donor nerve lesion, introduced in 1992 by Viterbo et al 17 , has been increasingly used [18][19][20][21][22][23][24][25][26][27][28][29][30][31][32] and has the advantage of not injuring the donor nerve.…”

Section: Introductionmentioning

confidence: 99%

Muscle reinnervation in one or two stages?: experimental study in rats with end-to-side nerve graft

et al. 2012

View full text Add to dashboard Cite

PURPOSE: To compare muscle reinnervation in one and two surgical stages using end-to-side neurorrhaphy (ESN) without donor nerve injury. METHODS: The experiment was performed on four groups of 20 rats. Group 1 (G1), one stage, received the graft which was sutured to the tibial nerve, with ESN, and its free stump was sutured end-to-end to the distal stump of the sectioned peroneal nerve (PN), all in the same operation. In Group 2 (G2), two stages, the nerve graft was sutured to the tibial nerve, with ESN. Two months later the PN was sectioned and its distal stump connected to the distal stump of the graft as in G1. Normal control group (Gn) received the graft only sutured to the tibial nerve, with ESN. Denervated control group (Gd), as well received the graft and had the PN sectioned and its two stumps buried in adjacent musculature, with the aim of denervating the cranial tibial muscle (CTM), the target of this study. The parameters used to evaluate CTM reinnervation were muscle mass, muscle fiber's minimum diameter and area. RESULTS: The mean CTM mass, the average of the muscular fibers areas and the average of the muscular fiber minimum diameters was higher (all p<0.0001) in G2 than in G1. Comparing the four groups, these parameters had their maximum expression in Gn and the minimum in Gd, as expected. CONCLUSION: The two stages showed better muscle reinnervation than one stage.

show abstract

“…Finally, stemming from observations of axonal fusion in invertebrates, highly innovative preclinical paradigms are currently being applied to mammalian systems, in which severed nerve fibers are surgically reconnected in 'end-to-end' or 'end-to-side' operations 37,38 . Together with the application of high molecular weight substances (such as polyethylene glycol) to the injury site that facilitate fusion and restoration of the original nerve tract 39-41 , these procedures have generated highly encouraging results with respect to functional recovery.…”

Section: Axonal Fusion During Regeneration Requires Conserved Componentsmentioning

confidence: 99%

The cellular and molecular mechanisms of axonal maintenance and regeneration

Coakley¹

View full text Add to dashboard Cite

How a neuron responds to and withstands injury is a central question in neurobiology. Neuronal injury can be caused mechanically with force, chemically with toxic insults, or genetically with mutations in genes that lead to damage. The axon, the neuron's longest compartment, is often the focal point of degeneration due to traumatic injury, toxic insults, or neurodegenerative diseases.Damage to the axon can have drastic functional consequences for the organism. In some cases axonal injury can lead to death of the neuron, while in others repair mechanisms are triggered that promote regeneration of the injured axon. Important discoveries during the last decade have uncovered many components of the molecular machinery that regulate axonal degeneration and regeneration. However, the precise ways in which these pathways converge and respond to different insults remains largely unknown. Here, using the nematode Caenorhabditis elegans as an experimental model system, we present important discoveries outlining the cellular and molecular mechanisms underpinning axonal maintenance and regeneration. Using forward and reverse genetic approaches, combined with optogenetic, genetic, and laser-injury tools, we identify critical components of these biological processes. We demonstrate how genes functioning during axon development also have roles in mature axons, regulating both degeneration and regeneration.Firstly, we discover a role for MEC-7/β-tubulin in regulating both axon development and regeneration. Second, we develop and characterize an optogenetic method to induce cell ablation and neurodegeneration using the genetically encoded photosensitizer KillerRed. Third, we identify novel mutants with defects in axonal maintenance and describe a new phenomenon of neuronal fusion following genetically induced damage. Finally, we demonstrate a role for conserved components of the apoptotic machinery in the recognition of damaged neurons during regeneration.These findings both extend our understanding of the molecular mechanisms of axonal degeneration and regeneration, as well as provide new paradigms in which these processes can be investigated.

show abstract

End-to-side nerve repair using fibrin glue in rats

Cited by 18 publications

References 17 publications

Nerve growth factor with fibrin glue in end-to-side nerve repair in rats

Nerve growth factor with fibrin glue in end-to-side nerve repair in rats

Muscle reinnervation in one or two stages?: experimental study in rats with end-to-side nerve graft

The cellular and molecular mechanisms of axonal maintenance and regeneration

Contact Info

Product

Resources

About