Experimental model of the formation of pelvic adhesions by videolaparoscopic in female rabbits

Abstract

Purpose:To verify the frequency of postsurgical pelvic adhesion formation in an experimental animal model using videolaparoscopy. Methods: Experimental study in a sample of 11 non-pregnant female rabbits, aged 5 to 7 months. After general anesthesia, access to the abdominal cavity was performed by an open puncture technique, with 10mm optics, placing two other 5 mm trochars under direct visualization, in the iliac fossae. Then a fragment of peritoneum was resected, followed by electrocauterization. In 21 days, the videolaparoscopy was repeated, and adhesion formation and score was looked at, with biopsies at the surgical site. Results: 54 % of adhesion formation was observed, and the median score of adhesions was 6 (minimum of 3 and maximum of 10), all of them found in the bladder and the anterior abdominal wall. Conclusion: The method used presents a high frequency of intra-abdominal adhesion formation. Key words: Adhesions. Laparoscopy. Surgery. Rabbits. RESUMOObjetivo: Verificar a freqüência da formação de aderências pélvicas pós-cirúrgicas, em um modelo experimental animal, por videolaparoscopia. Métodos: Estudo experimental, em uma amostra de 11 coelhas, não prenhas, com idade entre cinco e sete meses. Após anestesia geral, o acesso da cavidade abdominal foi efetuado por técnica de punção aberta, com óptica de 10 mm, colocando-se outros dois trocateres de 5 mm, sob visão direta, nas fossas ilíacas. Realizou-se, então, ressecção de fragmento de peritônio, seguida de cauterização com eletrocautério. Em 21 dias, foi repetida a videolaparoscopia, verificando-se a formação e escore de aderências e realizando-se biópsias do local da cirurgia. Resultados: Observou-se 54,5% de formação de aderências, sendo o escore total mediano de aderências seis (mínimo de três e máximo de 10), todas encontradas na bexiga e na parede abdominal anterior. Conclusão: O procedimento utilizado apresentou alta freqüência de formação de aderências intra-abdominais. Descritores: Aderências. Laparoscopia. Cirurgia. Coelhos.

Preventing post-surgical cardiac adhesions with a catechol-functionalized oxime hydrogel

“…The maximum adhesion intensity score was also reported for each animal. This is a common method used for adhesion scoring in various in vivo models [47][48][49] . Representative images of the observed adhesions in control and Ald-AO-Cat groups are shown in Fig.…”

“…Images from resternotomy were segmented into six equivalent sections (similar to the rat grading criteria), and each section was graded on a scale of 1-4 for adhesion coverage and strength as shown in Table 3. The scoring system was adapted from several surgical adhesion studies [47][48][49]59,67,68 .…”

Comparison of the effect of two anti-adhesion materials on the formation of postoperative adhesions after myomectomy – an experimental study on rabbit does

“…In an experiment involving rabbits, this theory was confirmed as adhesions formed in more than 50% of cases. These were adhesions to the anterior abdominal wall and the bladder (Balbinotto et al 2010).…”

Preventing post-surgical cardiac adhesions with a catechol-functionalized oxime hydrogel

“…This is a common method used for adhesion scoring in various in vivo models. [48][49][50] Representative images of the observed adhesions in control and Ald-AO-Cat groups are shown in Figure 4A. At 2 weeks, there was a significant reduction in average adhesion score when Ald-AO-Cat and Ald-AO were applied, compared to the untreated control ( Figure 4B).…”