Comparative study of technique to obtain stem cells from bone marrow collection between the iliac crest and the femoral epiphysis in rabbits

Abstract

Comparative study of technique to obtain stem cells from bone marrow collection between the iliac crest and the femoral epiphysis in rabbits 1Estudo comparativo da técnica para a obtenção de células tronco pela coleta de medula óssea entre a crista ilíaca e epífise do fêmur em coelhos ABSTRACT Purpose: To assess the technique for the collection of rabbit bone marrow stem cells from different regions to be used as an experimental model in regenerative medicine. Methods: Thirty rabbits were allocated into 2 groups: GROUP A, n=8, animals that underwent bone marrow blood (BMB) harvesting from the iliac crest; and GROUP B: including 22 rabbits that underwent BMB harvesting from the femur epiphysis. After harvesting, mononuclear cells were isolated by density gradient centrifugation (Ficoll -Histopaque). The number of mononuclear cells per ml was counted in a Neubauer chamber and cell viability was checked through Tripan Blue method. Results:Harvesting from the iliac crest yielded an average of 1 ml of BMB and 3,6.10 6 cells/ml over 1 hour of surgery, whereas an average of 3ml of BMB and 11,79.10 6 cells./ml were obtained in 30 min from the femur epiphysis with a reduced animal death rate. Conclusion:The analysis for the obtention of a larger number of mononuclear cells/ml from rabbit bone marrow blood was more satisfactory in the femur epiphysis than in the iliac crest.

Bone marrow cells of swine: Collection and separation

“…Access to the iliac crest of the swine was quite simple, however, there was a great resistance on the compact bone surface, requiring strong physical strength in the collection process, which led us to place all animals in the ventro lateral decubitus position, in contrast to Eça et al (2009) and Tognoli et al (2009), which suggest that the collection of bone marrow must be made in ventral decubitus. According to our assessment, this positioning could result in the force necessary to promote the joint injury or even fracture.…”

“…It consists of almost 5% of body weight, being responsible by the origin of blood cells (Müller et al, 2009). In adult animals, the canal central is replaced by fatty tissue, such that the active bone marrow is restricted only by the bone epiphysis, which have a high amount of cancellous bone (Eça et al, 2009).…”

Repair of critical‐size bone defects using bone marrow stromal cells: a histomorphometric study in rabbit calvaria. Part I: Use of fresh bone marrow or bone marrow mononuclear fraction

“…In this study, the bone marrow was harvested from the tibia because of the difficulty involved in obtaining more than 1 ml of bone marrow from the iliac crest (Eça et al. ). Even though harvesting bone marrow from the femur epiphysis area could allow an adequate amount of material, we had noticed that the tibial epiphysis access facilitates the procedure (data not shown).…”

Collagen fibrillar structures in vocal fold scarring and repair using stem cell therapy: a detailed histological, immunohistochemical and atomic force microscopy study

“…The bone marrow reserve of the femoral bone served as a source of autologous MSCs. The choice of the femoral bone was related to the great amount of the native cell material and its best quality, as compared to the other bones (Eça et al, 2009). The anaesthesia was conducted in the manner described in 'Creation of a defect in the vocal folds'.…”