Topic liver hypothermia and ischemic preconditioning: a new model of ischemia and reperfusion in rats

Abstract

The present model allows the induction of topical hepatic hypothermia associated or not to IPC. New studies to evaluate the possible synergistic effects of these tools can be reproduced without significant changes in macrohemodynamics and body temperature, or in other words, under stable conditions.

Topical hepatic hypothermia associated with ischemic preconditioning. Histopathological and biochemical analysis of ischemia reperfusion damage in a 24 hour model

“…2018;33(10):924-934 either method alone in an LIR model with extreme injury, monitoring intraoperative physiological parameters 19 and their possible histopathological 20 and biochemical 21 consequences measured at 24 hours of reperfusion after ischemic insult. The application of TH, alone or in conjunction with IPC, has already been evaluated by our group in an acute animal model with partial 90-minute liver ischemia followed by 120 minutes of reperfusion and immediate euthanasia, with the demonstration of its protective effects through the reduction of inflammatory markers such as tumor necrosis factor-α (TNF-α) and interleukin (IL)-1, IL-6, and IL-12; an increase in anti-inflammatory cytokines such as IL-10; increased levels of nitric oxide; and elevated expression of Nrf2, Keap1, and NQO1 genes; all are related to antioxidant effects [22][23][24] .…”