Polylactic acid film versus acellular porcine small intestinal submucosa mesh in peritoneal adhesion formation in rats

Costa, Ricardo Gonçalves da; Lontra, Marcelo Bentancor; Scalco, Priscila Pereira da Cunha; Cavazzola, Leandro Totti; Gurski, Richard Ricachenevsky

doi:10.1590/s0102-86502009000200010

Cited by 12 publications

(15 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, SIS has been used clinically as a safe material to repair vascular, urogenital and musculoskeletal tissues. SIS is a superior biomaterial due to its biodegradability, biocompatibility, and low rate of peritoneal adhesions (36). In this study, we generated a new scaffold containing both MSCs and SIS and investigated its effect on islets.…”

Section: Discussionmentioning

confidence: 99%

A new scaffold containing small intestinal submucosa and mesenchymal stem cells improves pancreatic islet function and survival in vitro and in vivo

Wang

Ding

Xue

et al. 2016

International Journal of Molecular Medicine

View full text Add to dashboard Cite

It is unknown whether a scaffold containing both small intestinal submucosa (SIS) and mesenchymal stem cells (MSCs) for transplantation may improve pancreatic islet function and survival. In this study, we examined the effects of a SIS-MSC scaffold on islet function and survival in vitro and in vivo. MSCs and pancreatic islets were isolated from Sprague-Dawley rats, and SIS was isolated from Bamei pigs. The islets were apportioned among 3 experimental groups as follows: SIS-islets, SIS-MSC-islets and control-islets. In vitro, islet function was measured by a glucose-stimulated insulin secretion test; cytokines in cultured supernatants were assessed by enzyme-linked immunosorbent assay; and gene expression was analyzed by reverse transcription-quantitative PCR. In vivo, islet transplantation was performed in rats, and graft function and survival were monitored by measuring the blood glucose levels. In vitro, the SIS-MSC scaffold was associated with improved islet viability and enhanced insulin secretion compared with the controls, as well as with the increased the expression of insulin 1 (Ins1), pancreatic and duodenal homeobox 1 (Pdx1), platelet endothelial cell adhesion molecule 1 [Pecam1; also known as cluster of differentiation 31 (CD31)] and vascular endothelial growth factor A (Vegfa) in the islets, increased growth factor secretion, and decreased tumor necrosis factor (TNF) secretion. In vivo, the SIS-MSC scaffold was associated with improved islet function and graft survival compared with the SIS and control groups. On the whole, our findings demonstrate that the SIS-MSC scaffold significantly improved pancreatic islet function and survival in vitro and in vivo. This improvement may be associated with the upregulation of insulin expression, the improvement of islet microcirculation and the secretion of cytokines.

show abstract

Section: Discussionmentioning

confidence: 99%

A new scaffold containing small intestinal submucosa and mesenchymal stem cells improves pancreatic islet function and survival in vitro and in vivo

Wang

Ding

Xue

et al. 2016

International Journal of Molecular Medicine

View full text Add to dashboard Cite

show abstract

“…Upon re-exploration, the integrity of the abdominal wall and gastrointestinal were verified. The Surgisis mesh was found intact and the bowel was easily dissected from the mesh as has been previously described in experimental models [27,28]. At operation, the patient was found to have extensive tumor necrosis from unresectable pelvic mass; because of potential compromised rectal wall, a loop diverting ostomy was created.…”

Section: Discussionmentioning

confidence: 99%

Early results on the use of biomaterials as adjuvant to abdominal wall closure following cytoreduction and hyperthermic intraperitoneal chemotherapy

2010

View full text Add to dashboard Cite

BackgroundHyperthermic chemotherapy applies thermal energy to both abdominal wall as well as the intra-abdominal viscera. The combination of the hyperthemia, chemotherapy and cytoreductive surgery (CRS) is associated with a defined risk of abdominal wall and intestinal morbidity reported to be as high as 15%, respectively to date, no studies have evaluated the use of biomaterial mesh as adjuvant to abdominal wall closure in this group of patients. In the present report, we hypothesized that post HIPEC closure with a biomaterial can reduce abdominal wall morbidity after CRS and hyperthermic intraperitoneal chemotherapy.Materials and methodsAll patients treated with HIPEC in a tertiary care center over 12 months (2008-2009) period were included. Eight patients received cytoreductive surgery followed by HIPEC for 90 minutes using Mitomycin C (15 mg q 45 minutes × 2). Abdominal wall closure was performed using Surgisis (Cook Biotech.) mesh in an underlay position with 3 cm fascial overlap-closure. Operative time, hospital length of stay (LOS) as well as postoperative outcome with special attention to abdominal wall and bowel morbidity were assessed.ResultsEight patients, mean age 59.7 ys (36-80) were treated according to the above protocol. The primary pathology was appendiceal mucinous adenocarcinoma (n = 3) colorectal cancer (n = 3), and ovarian cancer (n = 2). Four patients (50%) presented initially with abdominal wall morbidity including incisional ventral hernia (n = 3) and excessive abdominal wall metastatic implants (n = 1). The mean peritoneal cancer index (PCI) was 8.75. Twenty eight CRS were performed (3.5 CRS/patient). The mean operating time was 6 hours. Seven patients had no abdominal wall or bowel morbidity, the mean LOS for these patients was 8 days. During the follow up period (mean 6.3 months), one patient required exploratory laparotomy 2 weeks after surgery and subsequently developed an incisional hernia and enterocutaneous fistula.ConclusionThe use of biomaterial mesh in concert with HIPEC enables the repair of concomitant abdominal wall hernia and facilitates abdominal wall closure following the liberal resection of abdominal wall tumors. Biomaterial mesh prevents evisceration on repeat laparotomy and resists infection in immunocompromised patients even when associated with bowel resection.

show abstract

“…The presence of the polypropylene mesh may facilitate the formation of a fibrous matrix on its surface 1,3,7 . The adhesion of the bowel to the biomaterial is the first phase of bowel fistulization, which is considered more severe than the adhesions of the omentum 6 .…”

Section: Pp Phmentioning

confidence: 99%

“…In such cases, synthetic materials may provide a steady support for the restored wall 2 . However, intraperitoneal implanting of synthetic material may lead to dense adhesion of visceral organs, which may result in other complications 3 . Despite the great progress in surgical research on adopting strategies to prevent formation of adhesion due to intra-abdominal mesh implanting, it still represents a major post-surgical issue 4 .…”

Section: Introductionmentioning

confidence: 99%

2-hydroxyethyl methacrylate composite - polypropylene mesh for preventing peritoneal adhesions in female dogs

Silva¹,

Melo²,

Menezes³

et al. 2010

Acta Cir. Bras.

View full text Add to dashboard Cite

Purpose: To evaluate whether the lining facing the visceral side of polypropylene mesh made with 2-hydroxyethyl methacrylate (p(HEMA)) hydrogel could avoid peritoneal adhesion in female dogs. Methods: Eight animals (group PP) had a polypropylene mesh implanted to correct a defect in the rectal abdominal muscle, whereas in the other group (group PH) the polypropylene mesh was coated with p(HEMA) composite on the surface facing the peritoneal area. Results: Adhesions were observed on the mesh in 62.5% of the PP group. In the PH group adhesions were present only on the suture lines. Conclusion: p(HEMA) hydrogel was well tolerated and effective in avoiding visceral and omental adhesions on the surface of the polypropylene mesh.

show abstract

Polylactic acid film versus acellular porcine small intestinal submucosa mesh in peritoneal adhesion formation in rats

Cited by 12 publications

References 12 publications

A new scaffold containing small intestinal submucosa and mesenchymal stem cells improves pancreatic islet function and survival in vitro and in vivo

A new scaffold containing small intestinal submucosa and mesenchymal stem cells improves pancreatic islet function and survival in vitro and in vivo

Early results on the use of biomaterials as adjuvant to abdominal wall closure following cytoreduction and hyperthermic intraperitoneal chemotherapy

2-hydroxyethyl methacrylate composite - polypropylene mesh for preventing peritoneal adhesions in female dogs

Contact Info

Product

Resources

About