Brazilian law for scientific use of animals

The inhibition of maternal prolactin production in late lactation leads to metabolic syndrome and hypothyroidism in adult offspring. Physical training is a therapeutic strategy that could prevent or reverse this condition. We evaluated the effects of a short-duration low-intensity running wheel training program on the metabolic and hormonal alterations in rats. Lactating Wistar rats were treated with bromocriptine (Bro, 1 mg twice a day) or saline on days 19, 20, and 21 of lactation, and the training of offspring began at 35 days of age. Offspring were divided into sedentary and trained controls (C-Sed and C-Ex) and sedentary and trained Bro-treated rats (Bro-Sed and Bro-Ex). Chronic exercise delayed the onset of weight gain in Bro-Ex offspring, and the food intake did not change during the experimental period. At 180 days, visceral fat mass was higher (C46%) in the Bro-Sed offspring than in C-Sed and Bro-Ex rats. As expected, running capacity was higher in trained animals. Most parameters observed in the Bro-Sed offspring were consistent with hypothyroidism and metabolic syndrome and were reversed in the Bro-Ex group. Chronic exercise did not influence the muscle glycogen in the C-Ex group; however, liver glycogen was higher (C30%) in C-Ex group and was unchanged in both Bro offspring groups. Bro-Ex animals had higher plasma lactate dehydrogenase levels, indicating skeletal muscle damage and intolerance of the training program. Low-intensity chronic training is able to normalize many clinical aspects in Bro animals; however, these animals might have had a lower threshold for exercise adaptation than the control rats.

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“…11.794/2008 (Marques et al 2009)). Wistar rats at 120 days of age were kept at 25G1 8C on a dark-light cycle (0700 -1900 h).…”

Section: Methodsmentioning

confidence: 99%

Effects of running wheel training on adult obese rats programmed by maternal prolactin inhibition

Boaventura

Casimiro-Lopes

Pazos‐Moura

et al. 2013

Journal of Endocrinology

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“…After mating, each female was placed in an individual cage with free access to water and food until delivery. The use of animals was approved by the Animal Care and Use Committee of the Biology Institute of the State University of Rio de Janeiro (CEUA/016/2009) which based its analysis on the principles adopted and promulgated by Brazilian Law 11.794/2008, concerning the rearing and use of animals in teaching and research activities in Brazil (Marques et al 2009). …”

Section: Methodsmentioning

confidence: 99%

Effects of tobacco smoke exposure during lactation on nutritional and hormonal profiles in mothers and offspring

Santos-Silva

Oliveira²,

Pinheiro³

et al. 2011

Journal of Endocrinology

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Exposure to tobacco smoke is related to changes in energy balance regulation and several endocrine dysfunctions. Previously, we showed that maternal nicotine (the main addictive compound of tobacco) exposure exclusively during lactation affects biochemical profiles in mothers, milk, and pups. As the possible consequences for mothers and offspring of maternal smoking during lactation are still unknown, we evaluated the effects of tobacco smoke exposure on nutritional, biochemical, and hormonal parameters in dams and pups at weaning. After 72 h from birth, lactating rats were divided into two groups: smoke-exposed (S) in a cigarette-smoking machine, 4!1 h per day throughout the lactation period without pups; control (C), rats were treated the same as the experimental group but exposed to filtered air. Dams and pups were killed at weaning (21 days of lactation). Body weight and food intake were evaluated. Milk, blood, visceral fat, adrenal, and carcass were collected. S dams showed hyperprolactinemia (C50%), hypoinsulinemia (K40%), hypoleptinemia (K46%), as well as lower triglycerides (K53%) and very low-density lipoprotein cholesterol (K50%). Milk of S dams had higher lactose (C52%) and triglycerides (C78%). S pups presented higher body protein (C17%), lower total (K24%) and subcutaneous fat contents (K25%), hypoglycemia (K11%), hyperinsulinemia (C28%), hypocorticosteronemia (K40%), lower adrenal catecholamine content (K40%), hypertriglyceridemia (C34%), higher high-density lipoprotein cholesterol (C16%), and lower low-density lipoprotein cholesterol (K45%). In conclusion, tobacco smoke exposure leads to changes in nutritional, biochemical, and hormonal parameters in dams and, passively through the milk, may promote several important metabolic disorders in the progeny.

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“…Ethics -The use of the animals according to our experimental design was approved by the Animal Care and Use Committee of the Biology Institute of the State University of Rio de Janeiro (CEUA/232/2008), which based its analysis on the principles adopted and promulgated by Brazilian Law 11.794/2008 (Marques et al 2009). …”

Section: Methodsmentioning

confidence: 99%

The outcome of acute schistosomiasis infection in adult mice with postnatal exposure to maternal malnutrition

Corrêa

Lisboa

Oliveira

et al. 2011

Mem. Inst. Oswaldo Cruz

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Brazilian law for scientific use of animals

Cited by 97 publications

References 1 publication

Effects of running wheel training on adult obese rats programmed by maternal prolactin inhibition

Effects of running wheel training on adult obese rats programmed by maternal prolactin inhibition

Effects of tobacco smoke exposure during lactation on nutritional and hormonal profiles in mothers and offspring

The outcome of acute schistosomiasis infection in adult mice with postnatal exposure to maternal malnutrition

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