Cyclosporine action on kidneys of rats submitted to normothermic ischaemia and reperfusion

Abstract

PURPOSE: To verify if rat kidneys lesioned by ischaemia followed by reperfusion are affected by cyclosporine A (CsA). METHODS: Male Wistar rats were randomly divided into three groups, control (GS) and experimental (G1 and G2). G1 was subdivided in two: G1A composed of animals submitted to 60 minutes ischaemia and G1C with the same ischaemic procedure associated to 20 mg/kg/day CsA. Group G2 was subdivided and treated in the same way as G1 except that ischaemia was applied only for 40 minutes. Clamping the left renal artery followed by right side nephrectomy induced kidney ischaemia. Serum urea and creatinine were quantified on the day of surgery (D0) and in the following day (D1). Twenty four hours after reperfusion the left kidney was removed and histologically analyzed. RESULTS: Group GS had normal values for urea and creatinine both on D0 and D1 and did not show structural alterations. Renal function was not significantly different when G2C was compared to GS (p>0.05). Tissue lesions were smaller in G2C than in the other groups. CONCLUSIONS: Renal function was protected by CsA, which also reduced tissue lesions in the kidneys of rats submitted to 40 minutes ischaemia.

p66SHC-mediated mitochondrial dysfunction in renal proximal tubule cells during oxidative injury

“…At the applied concentration, CsA does not show cytotoxic effects in other proximal tubule cell line, either (50). In addition, protective effects of CsA against oxidative damage have also been reported in vitro (15,20,24) and in vivo (13,33,39).…”

Histopathological Study of Cyclosporine Pulmonary Toxicity in Rats

“…They were divided into three groups; each group consists of twenty rats. The first group (control) received physiological saline only while the second received 25 mg/kg/day of cyclosporine dissolved in physiological saline [ 6 ], whereas the third group received 40 mg/kg/day of cyclosporine dissolved in physiological saline [ 7 ]. Daily administration of physiological saline and cyclosporine was done for forty-five days by using gastric gavage whereas cyclosporine was available in a soft gelatin capsule (50 mg) that was manufactured by R. P. Scherer GmbH & Co. KG, Eberbach/Baden, Germany, for Novartis Pharma AG, Basel, Switzerland.…”

Renoprotective Effects of Naringenin and Olive Oil against Cyclosporine- Induced Nephrotoxicity in Rats

“…Rats were divided into four groups (n=20 each one). The control group received physiological saline, the second group treated with 25 mg/kg/d of cyclosporine dissolved in physiological saline [15], while the third and fourth groups received 100 mg/kg/d of naringenin [16], and 1.25 ml /kg /d of virgin olive oil [17], respectively, together with the same cyclosporine dose. Cyclosporine, naringenin and virgin olive oil doses were given daily by gastric gavage.…”