Effects of preoperative pelvic irradiation on colonic anastomosis healing: an experimental study in rats

França, A Spina; Ramalho, Fernando Silva; Ramalho, Leandra Naíra Zambelli; Rocha, José Joaquim Ribeiro da; Féres, Omar

doi:10.1590/s0102-86502008000700005

Cited by 5 publications

(5 citation statements)

References 43 publications

(52 reference statements)

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“…(10)(11)(12) Risk factors for anastomotic dehiscence include malnutrition, anaemia, local infections, obesity, female gender, intraoperative hypotension, emergent surgery and disrupted blood supply, as well as preoperative neoadjuvant therapies. (10,11,(13)(14)(15)(16)(17)(18)(19) Melatonin has been reported to have favourable effects on wound healing. (20) However, it is unclear whether melatonin can alleviate the potential side effects of preoperative chemotherapy on wound healing.…”

Section: Introductionmentioning

confidence: 99%

Effects of melatonin on colonic anastomosis healing following chemotherapy in rats

Akyüz

Yaşar

Uzun

et al. 2018

smedj

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Section: Introductionmentioning

confidence: 99%

Effects of melatonin on colonic anastomosis healing following chemotherapy in rats

Akyüz

Yaşar

Uzun

et al. 2018

smedj

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“…The development of new effective radiation techniques is essential for the expansion of such research, and we described here an effective way of irradiating the pelvis without compromising machines used for cancer patients' treatment 11,12 .…”

Section: ■ Discussionmentioning

confidence: 99%

Development of a new model of actinic enteritis in rats using a cobalt-60 open source and a protection device as a collimator

et al. 2017

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IVDevelopment of a new model of actinic enteritis in rats using a cobalt-60 open source and a protection device as a collimator 1 7-Technical SkillActa Cir Bras. 2017;32(3):319-324 AbstractPurpose: To describe a new model of actinic enteritis that does not use radiotherapy machines. Methods: Sixteen Wistar rats were divided into four groups, consisting of four animals each: control (group A), two weeks after irradiation (group B), five weeks after irradiation (group C) and eight weeks after irradiation (group D). Animals were given a 10Gy radiation from a Cobalt-60 natural source in a nuclear technology research center. Protections of the surrounding tissues were obtained through the usage of plumb devices with a hole in the center, which served as a collimator. We obtained irradiated and non-irradiated colons from each animal.Results: In group B we found an important inflammatory response in the irradiated colon, which appeared in a reduced way in group C and was minimal in group D, in which we found a relevant collagen submucosal deposition/fibrosis. In all groups, the non-irradiated colon had a lower pathological damage in comparison with the irradiated colon. Conclusion: We thus described an efficient and feasible technique for obtaining an animal model of actinic enteritis. around 220-280g and aged 2-3 months were used in this study, obtained from the Universidade Federal de Minas Gerais (UFMG). The rats were housed in polycarbonate cages (49×34×16cm), with n= 4/cage, under controlled conditions (temperature, humidity, air flux). Throughout the experimental period, all mice had access to food (Purina Lab Chow, Curitiba, Brazil) and filtered water ad libitum.The rats were randomly distributed into four groups of four animals each: group A (control without irradiation); group B (single dose 10Gy irradiation and euthanized after two weeks); group C (single dose 10Gy irradiation and euthanized after five weeks); group D (single dose 10Gy irradiation and euthanized after eight weeks).All experimental procedures were performed on anesthetized rats. Anesthesia was maintained with ketamine and xylazine (60mg/Kg and 8mg/Kg i.p.). After properly anesthetized, the rats were set in an acrylic cylindrical compartment (Figure 1). They were transferred into a protected room which has the MDS Nordion Cobalt-60 source in the center. This room has a complex protection mechanism that rises the source from an enclosed cage from under the ground only when it is fully locked and safe. We developed a covering device (15cm-thick plumb bricks with a 32mm round opening in the center to provide a collimator) to protect the rest of the animal's body from the radiation shade (Figure 2). This protective plumb device did not cover the back of the animal, in order to allow radiation to disperse after collimation and prevent it from reflecting and spreading in the surrounding tissues (Figure 3). A TLD dosimetry system was used in the pilot experiment so as to check the proper radiation dose in the irradiated field and it also proved that ...

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“…On the twenty-eighth day, all of the animals were subjected to transection and anastomosis of the left colon and received a subcutaneous injection of either vitamin K (cirrhosis group) or saline (control group), as previously described 8 . On the thirty-fifth day (seven days after the anastomosis), the animals were sacrificed, and their abdominal cavities were evaluated macroscopically for the presence of anastomotic dehiscence and peritonitis.…”

Section: Methodsmentioning

confidence: 99%

“…Certain systemic conditions, such as hypotension, hypovolemia, intestinal ischemia, low oxygen tension, advanced malignant neoplasia, dehydration, uremia, age and malnutrition, can affect healing [8][9][10][11][12][13][14] . Some studies have shown a higher incidence of colonic anastomotic dehiscence in patients with intraoperative hypotension, anemia, and cirrhosis 1,6 .…”

Section: Introductionmentioning

confidence: 99%

Liver cirrhosis on the colonic anastomotic healing in rats

Bonifácio¹,

Parra²,

Almeida³

et al. 2011

Acta Cir. Bras.

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PURPOSE:To investigate the effects of cirrhosis on colonic anastomosis healing in rats. METHODS:Fifty five Wistar male rats were used (23 in the control group and 32 in the cirrhosis group). On the first day of the procedure, the rats in the cirrhosis group underwent double ligation and folding of the common bile duct to induce liver cirrhosis, and the control rats underwent a laparotomy and intestinal manipulation. On the fourteenth and thirty-fifth days, all of the animals were biochemically assessed for serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase, bilirubin, total protein, and albumin and for liver histopathology. On the thirty-fifth day, cirrhosis was confirmed. On the twenty-eighth day, all of the animals were subjected to left colon transection and anastomosis. On the seventh day after the colonic anastomosis, the rats were sacrificed and macroscopically evaluated for dehiscence. The region of the colonic anastomosis was removed and subjected to hydroxyproline content measurement, conventional histology, and the immunohistochemical determination of vascular endothelial growth factor (VEGF) and matrix metalloproteinase type 1 (MMP 1). RESULTS:The biochemical and histopathological examinations confirmed cirrhosis in all of the animals in the cirrhosis group. More deaths occurred after anastomosis in the cirrhosis group (5/25) than in the control group (0/21), and anastomotic dehiscence was more frequent in the cirrhosis group (8/25) than in the control group (0/21). The average hydroxyproline concentration was lower in the cirrhosis group than in the control group. The immunohistochemical studies showed that the average VEGF expression in the cirrhosis group was lower than in the control group, and the average MMP1 expression was higher in the cirrhosis group. CONCLUSION:Hepatic cirrhosis leads to increased mortality and colonic anastomotic dehiscence, an increased distance between the mucosal edges of the anastomosis area, and a lower hydroxyproline concentration in the colonic anastomosis; therefore, these conditions are deleterious to healing. cirrose foi confirmada. No dia 28 todos os animais foram submetidos à colectomia esquerda e anastomose. 70 dias após anastomose os ratos foram submetidos à eutanásia e foram avaliados macroscopicamente a procura de deiscência. A região da anastomose colônica foi removida para dosagem de hidroxiprolina, histologia convencional e imunohistoquímica para determinação do fator de crescimento endotelial vascular (VEGF) e metaloproteinase tipo 1 (MMP 1). RESULTADOS:A análise bioquímica e histológica confirmou a cirrose em todos os animais do grupo cirrose. Óbito ocorreu em maior freqüência após a anastomose no grupo cirrose (5/25) se comparado com grupo controle (0/21), e a deiscência da anastomose foi mais freqüente no grupo cirrose (8/25) se comparado com controle (0/21). A concentração média de hidroxiprolina foi menor no grupo cirrose se comparado com grupo controle. A análise imonuhistoquímica mostrou que a exp...

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Effects of preoperative pelvic irradiation on colonic anastomosis healing: an experimental study in rats

Cited by 5 publications

References 43 publications

Effects of melatonin on colonic anastomosis healing following chemotherapy in rats

Effects of melatonin on colonic anastomosis healing following chemotherapy in rats

Development of a new model of actinic enteritis in rats using a cobalt-60 open source and a protection device as a collimator

Liver cirrhosis on the colonic anastomotic healing in rats

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