Apoptotic effects of inositol hexaphosphate on biomarker Itpr3 in induced colon rat carcinogenesis

Marks, Guido; Fagundes, Djalma José; Ynouye, Celso Massaschi; Pontes, Elenir Rose Jardim Cury; Takita, Luiz Carlos; Amaral, Eva Glória Siufi do; Teruya, Roberto; Paes, Manoel Catarino; Brasileiro, José Lacerda; Aydos, Ricardo Dutra

doi:10.1590/s0102-86502008000200008

Cited by 3 publications

(2 citation statements)

References 17 publications

(4 reference statements)

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“…It is reported that phytate has multiple biological functions including anticancer activity 2, 3. Numerous animal studies have shown that dietary supplementation with phytate provides substantial protection against experimentally induced colonic cancer 4, 5. It was reported that administration of phytate suppressed the aberrant crypt foci formation 5 and induced the cellular apoptosis 4 in rat model of colon cancer induced by azoxymethane.…”

Section: Introductionmentioning

confidence: 99%

“…Numerous animal studies have shown that dietary supplementation with phytate provides substantial protection against experimentally induced colonic cancer 4, 5. It was reported that administration of phytate suppressed the aberrant crypt foci formation 5 and induced the cellular apoptosis 4 in rat model of colon cancer induced by azoxymethane. Furthermore, in vitro studies using cultured cancer cells have been conducted with phytate itself to elucidate the molecular mechanisms underlying the phytate‐mediated anticancer action 6, 7.…”

Section: Introductionmentioning

confidence: 99%

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Phytate hydrolysate induces circumferential F‐actin ring formation at cell–cell contacts by a Rho‐associated kinase‐dependent mechanism in colorectal cancer HT‐29 cells

Suzuki

Hara

2010

Molecular Nutrition Food Res

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Phytate (inositol hexa-phosphate or IP6) possessing anticancer activity is hydrolyzed by phytase in intestinal microbes and the metabolites are distributed throughout the colon. Cellular circumferential F-actin rings, which are involved in cell polarity and structure, are lost early during tumorigenesis. We investigated F-actin ring formation by the phytate hydrolysate in colorectal cancer HT-29 cells to explore the novel mechanisms underlying the phytate-mediated anticancer function. The phytate hydrolysate, but not inositol or phytate, induced F-actin ring formation with a peak at 10 min in the cells and was associated with phosphorylation of myosin regulatory light chain. F-actin ring formation and myosin regulatory light chain phosphorylation by the phytate hydrolysate were suppressed by inhibitors of Rho-associated kinase (ROCK), Janus kinase (JAK), c-Jun N-terminal kinase (JNK), and protein kinase Cδ (PKCδ). Activation of ROCK and JAK, but not JNK or PKCδ, was observed at 10 min and/or earlier after stimulation with the phytate hydrolysate. Altogether, the phytate hydrolysate induces circumferential F-actin ring formation through a ROCK-dependent myosin II activation in the HT-29 cells, which requires JAK activation and basal activities of JNK and PKC. Hydrolysis products of phytate in the intestine may contribute to anticancer function of phytate.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Phytate hydrolysate induces circumferential F‐actin ring formation at cell–cell contacts by a Rho‐associated kinase‐dependent mechanism in colorectal cancer HT‐29 cells

Suzuki

Hara

2010

Molecular Nutrition Food Res

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The role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of rats

et al. 2010

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PURPOSE: To study the protein Fas ligand (FasL) on the expression of apoptosis, using a model of oxidative stress induced by azoxymethane (AOM), in the crypt of colon in rats. METHODS: Wistar rats (n=14) were assigned into two groups: control (n=7) and AOM (n=7). A single subcutaneous administration of AOM (5mg/kg) or saline solution was performed at the beginning of third week and after three hours samples of proximal colon were collected. The expression of FasL was quantified (Software ImageLab) in percentage of areas in the top, base and all crypt. Results were expressed as mean ± sd (Shapiro-Wilks test and t Student test) (p < 0.05). RESULTS: In the animals of CG there was no significant difference between the FasL expression of the top (10.75±3.33) and basal (11.14±3.53) colon crypt (p=0.34293740). In the animals of AOM there was no significant difference between the FasL expression of the top (8.86±4.19) and basal (8.99±4.08) colon crypt (p=0.78486003). In the animals of CG (10.95±3.43) and AOM (8.92±4.13) there was a significant difference of the FasL expression (p=0.026466821). A significantly decrease on the FasL expression was observed in the animals of CG (10.75±3.33) and AOM (8.86±4.19) in the top crypt (p=0.00003755*). A significant decrease was also observed in the animals of CG (11.14±3.53) and AOM (8.99±4.08) in the basal colon crypt (p=0.00000381**). CONCLUSION: Azoxymethane induce the oxidative stress and the significantly decrease of FasL expression, although there is no significant difference between basal and top of colon crypt linked to consumption-activation of Fas ligand.

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Pentoxifylline and prostaglandin E1 action on ischemia and reperfusion of small intestine tissue in rats. An immunohistochemical study

Brasileiro

Ramalho²,

Aydos

et al. 2015

Acta Cir. Bras.

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PURPOSE:To investigate the action of pentoxifylline (PTX) and prostaglandin E1 (PGE1) on ischemia and reperfusion of small intestine tissue in rats, using immunohistochemical analysis. METHODS:Thirty-five Wistar rats were distributed as follows: group A (n=10): subjected to intestinal ischemia and reperfusion for 60 min, with no drugs; group B (n=10): PTX given during tissue ischemia and reperfusion; group C (n=10): PGE1 given during tissue ischemia and reperfusion; group D (n=5): sham. A segment of the small intestine was excised from each euthanized animal and subjected to immunohistochemical examination. RESULTS:Mean number of cells expressing anti-FAS ligand in the crypts was highest in Group A (78.9 ± 17.3), followed by groups B (16.7 ± 2.8), C (11.3 ± 1.8), and D (2.5 ± 0.9), with very significant differences between groups (p<0.0001). CONCLUSIONS:The use of pentoxifylline or prostaglandin E1 proved beneficial during tissue reperfusion. The immunohistochemical results demonstrated a decrease in apoptotic cells, while protecting other intestinal epithelium cells against death after reperfusion, allowing these cells to renew the epithelial tissue.

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Apoptotic effects of inositol hexaphosphate on biomarker Itpr3 in induced colon rat carcinogenesis

Cited by 3 publications

References 17 publications

Phytate hydrolysate induces circumferential F‐actin ring formation at cell–cell contacts by a Rho‐associated kinase‐dependent mechanism in colorectal cancer HT‐29 cells

Phytate hydrolysate induces circumferential F‐actin ring formation at cell–cell contacts by a Rho‐associated kinase‐dependent mechanism in colorectal cancer HT‐29 cells

The role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of rats

Pentoxifylline and prostaglandin E1 action on ischemia and reperfusion of small intestine tissue in rats. An immunohistochemical study

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