Experimental model of severe acute pancreatitis in rabbits

Abstract

Purpose:To develop an experimental model of severe acute pancreatitis in rabbits through a pancreatic ductal injection of sodium taurocholate. Methods: Twenty-four albino rabbits of the New Zealand lineage were distributed into four groups of six animals (A, B, C and S). The rabbits of three experimental groups (A, B and C) were submitted to a laparatomy and received a pancreatic ductal injection of 1ml/kg sodium taurocholate 5%. Also, they were submitted to further laparatomies after 4h, 8h and 12h, respectively. The control group (S) was subdivided into two groups of three animals: in subgroup S1 only the pancreatic duct catheterization was performed whereas in subgroup S2 the pancreatic duct catheterization as well as an injection of 1ml/kg physiologic solution 0.9% were carried out. After 12 hours, the rabbits were evaluated. In the re-intervention, blood was collected to determine the amylasemia and a pancreatectomy was carried out to investigate interstitial infiltration, steatonecrosis and necrosis of the organ, using an optical microscope. Results: There was an elevation of amylase in all groups thus proving the existence of acute pancreatitis. The size of the interlobular septum increased progressively with a greater variation between group S1 (0.13) and group C (0. 53) (p=0.035). While all the animals in group A exhibited focal cellular necrosis, it was more intense in the rabbits of group B and culminated with a high proportion of severe pancreatic necrosis in group C animals. The difference in the intensity of cellular necrosis showed statistic significance (p=0.001). Conclusion: The proposed experimental model demonstrated its reproducibility and effectiveness in producing severe acute pancreatitis in rabbits. Key words: Pancreatitis, Acute Necrotizing. Animal Experimentation. Rabbits. RESUMOObjetivo: Desenvolver modelo experimental de pancreatite aguda grave em coelhos por meio da injeção de taurocolato de sódio no ducto pancreático. Métodos: Vinte e quatro coelhos albinos da linhagem Nova Zelândia foram distribuídos em quatro grupos de seis animais (A, B, C e S). Os coelhos dos três grupos experimentais (A, B e C) foram submetidos a laparotomia e injetou-se taurocolato de sódio a 5%, 1ml/Kg no ducto pancreático. Realizou-se nova laparotomia, respectivamente, após 4h, 8h e 12h. No grupo controle (S), subdividido em dois grupos de três animais, foi realizada no subgrupo S1 apenas cateterização do ducto pancreático e no subgrupo S2 cateterização do ducto pancreático e injeção de solução fisiológica 0,9%, 1ml/Kg. Estes animais foram reavaliados após 12 horas. Na reintervenção coletou-se sangue para determinação da amilasemia e realizou-se pancreatectomia para análise histológica do infiltrado intersticial, da esteatonecrose e da necrose do órgão. Resultados: Houve elevação da amilase em todos os grupos, demonstrando a presença da pancreatite aguda. O tamanho do septo interlobular aumentou progressivamente, observando-se maior diferença entre os grupos S1 (0,13) e C (0,53) (p=0,035). Todos os ...

Protective Effects of Saizen in Combination With Stilamin on Intestinal Mucosa of a Rabbit Model of Severe Acute Pancreatitis

“…The rabbits were anesthetized by injection of pentobarbital (1 mL/kg). Briefly, 19 under aseptic conditions, a median abdominal wall incision was made to expose duodenal papilla, then found the opening of pancreatic duct, and a 0.5-mm scalp needle was used to prick a small hole. Animal models were made by 5% sodium taurocholate retrograde pancreatic duct puncture injection at constant speed, and the site of puncture was clamped with injury-free blood vessel forceps to avoid leakage of taurocholate, and they were removed 30 minutes later.…”

Η σημασία της θεραπείας με b-d γλυκάνη στην επιμόλυνση του νεκρωμένου παγκρεατικού ιστού από Candida Albicans σε πειραματικό μοντέλο νεκρωτικής παγκρεατίτιδας

“…55 Από την άλλη πλευρά, το κουνέλι είναι ζώο μεσαίου μεγέθους, καθιστώντας δυνατή την λήψη μεγαλύτερης ποσότητας αίματος για βιοχημική αξιολόγηση και μέτρηση προφλεγμονωδών κυτταροκίνων. 66 Λόγω της μεγάλης απόστασης των εκβολών χοληδόχου και παγκρεατικού πόρου εντός του λεπτού εντέρου, σε αντίθεση με τα περισσότερα θηλαστικά, το πείραμα της απόφραξης της χολοπαγκρεατικής συμβολής καθίσταται αδύνατο. Έγχυση χυνοδεοξυχολικού και ταυροχολικού οξέος στον παγκρεατικό πόρο, με ή χωρίς απολίνωση αυτού προκαλούν σοβαρή παγκρεατίτιδα στα πειραματόζωα.…”