Effects of acetylsalicylic acid and acetic acid solutions in VX2 carcinoma cells: In vitro analysis

Abstract

PURPOSE: To analyze, in vitro, the effects of acetylsalicylic acid (aspirin) and acetic acid solutions on VX2 carcinoma cells in suspension and to examine the correlation between these effects and neoplastic cell death. METHODS: The VX2 tumor cells (10(7) cells/ml) were incubated in solutions containing differing concentrations (2.5% and 5%) of either acetylsalicylic acid or acetic acid, or in saline solution (controls). Every five minutes, cell viability was tested (using the trypan blue test) and analyzed under light microscopy. RESULTS: Tumor cell viability (in %) decreased progressively and, by 30 minutes, neoplastic cell death had occurred in all solutions. CONCLUSION: Based on this experimental model and the methodology employed, we conclude that these solutions cause neoplastic cell death in vitro.

Evaluation of peritoneal endometriosis treatment using intralesional acetylsalicylic acid injection in rabbits

“…In this context, it is of paramount importance to acknowledge the definite need for the development of new drugs to provide specific and more efficient therapeutic alternatives to completely eliminate endometriotic lesions, prevent recurrences, and has none or low side effects 6 . Therefore, based on our previously tested different doses and periods of time in other experimental rabbit models [7][8][9][10][11] , the present study was delineated to evaluate whether a higher aspirin concentration and longer follow-up of outcomes (namely, 20% aspirin and 20 days after intralesional injection, respectively) could positively impact on completely reducing peritoneal endometrial tissue.…”

“…Over the last decade, our research group [7][8][9][10][11][12] Therefore, the present study assessed the therapeutic efficacy of intralesional 20% aspirin injection using the wellestablished peritoneal endometriosis rabbit model.…”

Effects of aspirin on mesenteric lymph nodes of rabbits as basis for its use on lymph nodes metastases

“…This ability to destroy tumor cells has already been investigated by our group [6][7][8][9][10] in other reports using experimental tumors (Ehrlich and VX-2) and by others 27,[31][32][33][34][35][36][37][38] .…”

“…In our research group, we had the opportunity to study and scientifically prove cytolytic effects of aspirin (acetylsalicylic acid) and its derivatives, either in vitro or in vivo on several experimental models [6][7][8][9][10] . Following this line of research, the purpose of this experimental study was to evaluate the effects of aspirin on normal lymph nodes of rabbits as basis for its later use on lymph nodes affected by tumor.…”

Anti-tumour effects of transcatheter arterial embolisation administered in combination with thalidomide in a rabbit VX2 liver tumour model

“…VX2 tumour cells derived from rabbits were provided by the Cell Resource Center for Biomedical Research, Institute of Development, Aging and Cancer, Tohoku University. They were maintained in Dulbecco's modified Eagle medium (Nacalai Tesque Inc., Kyoto, Japan) supplemented with L-glutamine, 5% foetal bovine serum (FBS) (Gibco, New York, NY) and 1% penicillin/streptomycin (Sigma, Aldrich, St Louis, MO) [12].…”