Efeitos da desnervação intrínseca do jejuno após enterectomia extensa na síndrome do intestino curto em ratos

Carvalho, Carlos Eduardo Verdiani de; Basile, F; Vespúcio, Marcelo Vinícius Oliveira; Iglesias, Antonio; Gava, Nelson Fabrício; Garcia, Sérgio Britto

doi:10.1590/s0102-86502006000100010

Cited by 3 publications

(3 citation statements)

References 10 publications

(10 reference statements)

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“…Thus, this study brings the possibility of minimizing the rates of adverse complications because of intramural microinjections, which does not lead to direct contact of CB with adjacent organs and abdominal cavity. The reduction in the number of myenteric neurons found in this study corroborates the findings of other studies (Sato et al 1978, Sakata et al 1979, Garcia et al 1999, Carvalho et al 2006, Fujimura et al 2016, Yu et al 2016, Belkind-Gerson et al 2017 as the application of CB in serosa of the intestinal segment provoked myenteric denervation. In our study, it was possible to promote such change with the use of the technique of intramural microinjections, being effective in the myenteric denervation with minor complications, since peritonitis or small bowel adhesions were not observed.…”

Section: Discussionsupporting

confidence: 92%

“…Chemical denervation by benzalkonium chloride as an experimental model of the digestive tube has been studied in different ways in the pathophysiology enteric coated, for example, for studies of the role of the enteric nervous system in proliferation mucosal epithelial cell and control of peristalsis (Zucoloto et al 1991, Garcia et al 1999, Buttow et al 2003, Carvalho et al 2006). There is a possibility that the benzalkonium chloride (CB) causes intrinsic denervation of the intestine (Oliveira et al 1990, Hanani et al 2003, Laranjeira et al 2011, Fujimura et al 2016, Yu et al 2016) and possibly has a clinical application in situations where denervation is a recommended for specific segments.…”

Section: Introductionmentioning

confidence: 99%

“…There is a possibility that the benzalkonium chloride (CB) causes intrinsic denervation of the intestine (Oliveira et al 1990, Hanani et al 2003, Laranjeira et al 2011, Fujimura et al 2016, Yu et al 2016) and possibly has a clinical application in situations where denervation is a recommended for specific segments. An example is the denervation of the remaining ileum in Short Bowel Syndrome in experimental rats, in which it has a beneficial effect on animal clinical parameters, therefore constituting a possibility of treatment in the future (Garcia et al 1999, Carvalho et al 2006. However, there is a need to improve the techniques of application of CB, because in the existing model, the application in the intestine serosa through laparotomy can lead to peritonitis and formation of bands, which lead to a high mortality.…”

Section: Introductionmentioning

confidence: 99%

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A new experimental model of intrinsic denervation in ileum from wistar rats through intramural microinjections of benzalkonium chloride

et al. 2019

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Extensive literature is available about the intrinsic denervation of segments of the digestive tube through the application of CB in the serosa of the viscera. However, this technique has some disadvantages like causing peritonitis, flanges and high mortality, limiting its use in humans. The aim of the present study was to evaluate the feasibility of benzalkonium chloride (CB) to induce intrinsic chemical denervation, through applications of CB in the intramural ileum of wistar rats, as well as deepen the knowledge about the evolution of neuronal injury caused in the process. We used 40 rats, divided into two groups (control-GC and benzalkonium-GB) of 20 animals each, divided into four sub-groups according to the time of postoperative assessment of 24, 48 hours, 30 and 90 days. The animals were submitted to intramural microinjections of sterile saline solution 0.9% (GC) or benzalkonium chloride (GB) in ileal portion, and subsequent histopathological analysis and immunohistochemistry for evaluation of neuronal injury. A significant decrease (p<0.05) was found of the neuronal myenteric count over time in groups, GB3, GB4 and GB2. The specific positive immunolabeling for H2AX and Caspase-3 confirmed the results obtained in the histopathological evaluation, denoting the ignition of irreversible cell injury in 24 hours, evolving into neuronal apoptosis in 48 hours after application of the CB 0.3%. Under the conditions in which this work was conducted, it can be concluded that the application of CB 0.3% by means of microinjections intramural in the ileal wall is able to induce intrinsic chemical denervation of the diverticulum of wistar rats and that the main mechanism of neuronal death is induction of apoptosis.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A new experimental model of intrinsic denervation in ileum from wistar rats through intramural microinjections of benzalkonium chloride

et al. 2019

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Myenteric Denervation of the Gut with Benzalkonium Chloride: A Review of Forty Years of an Experimental Model

Garcia

Minto

Marques

et al. 2019

Canadian Journal of Gastroenterology and Hepatology

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Experimental denervation of organs plays a key role in understanding the functional aspects of the normal innervation as well as the diseases related to them. In 1978 the experimental model of myenteric denervation of the rat gut by serosal application of benzalkonium chloride (BAC) was proposed. BAC is a positively charged surface-active alkylamine and is a powerful cationic detergent, which destroys bacteria after ionic attraction and for this reason is largely used as a surgical antiseptic. Since its initial report, the BAC-induced myenteric denervation model has been used to study many functional and pathological aspects of the enteric nervous system. So far this is the only pure method of myenteric denervation available for research in this area. Promising reports in the literature have shed light on the possibilities for the development of new uses of the BAC-denervation experimental model as a therapeutic tool in some pathological situations. This review aims to shed light on the main historical and recent findings provided by this experimental model.

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Botulinum toxin as a treatment for short bowel syndrome in rats

Marques

Minto²,

Marques³

et al. 2019

Acta Cir. Bras.

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The denervation of the intestine with benzalkonium chloride (BAC) reduces mortality and improves weight gain in rats with short bowel syndrome (SBS). Nevertheless, translating these promising findings from bench to bedside is not feasible because BAC promotes peritonitis and irreversible denervation which may be followed by an uncontrolled dilatation of the viscera. The use of botulinum toxin (BT) instead of BAC to achieve the denervation of the remaining small intestine in SBS could be an interesting option because it leads to a mild and transient denervation of the intestine. Methods: Here we evaluated the effects of the ileal denervation with BT in rats with SBS by verifying the body weight variation and intestinal morphological parameters. Four groups with 6 animals each were submitted to enterectomy with an ileal injection of saline (group E) or BT (group EBT). Control groups were submitted to simulated surgery with an ileal injection of BT (group BT) or saline (group C-control). Results: We observed that the treatment of the remaining ileum with BT completely reversed the weight loss associated to extensive small bowel resection. Conclusion: This may provide a new promising approach to the surgical treatment of SBS.

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Efeitos da desnervação intrínseca do jejuno após enterectomia extensa na síndrome do intestino curto em ratos

Abstract: The denervated animals presented an increase the body weight gain and mucosal cell proliferation responses when compared to the control group. This experimental model may provide new strategies for the surgical treatment of the short bowel syndrome.

Cited by 3 publications

References 10 publications

A new experimental model of intrinsic denervation in ileum from wistar rats through intramural microinjections of benzalkonium chloride

A new experimental model of intrinsic denervation in ileum from wistar rats through intramural microinjections of benzalkonium chloride

Myenteric Denervation of the Gut with Benzalkonium Chloride: A Review of Forty Years of an Experimental Model

Botulinum toxin as a treatment for short bowel syndrome in rats

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