Experimental model of gastroesophageal reflux in rats

Abstract

Purpose:To develop an experimental model of gastroesophageal reflux in rats. Methods: Sixty Wistar rats underwent surgery and were assigned to one of the three groups of twenty animals each. The animals in group A underwent total esophageal myectomy and, in group (B), underwent partial myectomy. The third group was the control group (C). A contrast radiographic study of the esophagus was performed to evaluate gastroesophageal reflux. The anatomopathological study of the esophagus was used to evaluate esophagitis. Results: During the 30-day postoperative follow-up, 14 animals in group A presented with reflux of barium in the esophagus. The presence of barium in the esophagus was observed in 7 animals in group B and in 2 animals in the control group. The result of the histopathology examination was controversial. A marked weight loss in the rats undergoing total myectomy was observed, however there was no significant statistical difference. Conclusion: Total myectomy in the lower third of the esophagus caused gastroesophageal reflux in the majority of the animals.

Expression of claudin-3 in the esophagus and larynx of rat reflux model

“…Gaia Filho et al [18] found that a total myectomy with a 1.5-cm muscle layer resection of the abdominal esophagus causes reflux in rats, proving to be a good experimental model. Shimazu et al [19] showed that induced chronic reflux esophagitis resulted in histological changes of the pharynx and the larynx in a rat model.…”

“…We used the surgical procedure to achieve experimental reflux in the literature before [12]. The animals were fastened for 12 h prior to surgery.…”

Histologic Changes in Eustachian Tube Mucosa of Rats After Exposure to Gastric Reflux

“…A 2‐cm midline laparotomy incision was made, starting at the xiphoid process, and the peritoneal cavity was inspected. GER was induced using the upper abdominal midline pyloric stenosis plus limiting ridge ligation method 3 . The pyloric stenosis involved covering the duodenum near the pyloric ring with a small piece of an 18 Fr Nelaton catheter while the transitional region between the forestomach and the glandular portion (limiting ridge) was ligated using a nonabsorbable suture.…”

An Obese Rat Model of Bariatric Surgery with Gastric Banding

“…The lower part, which is similar to the human stomach (glandular stomach) is the pyloric or glandular region. 17,18 Because of these differences, it is very difficult to perform a gastric banding operation equivalent to that in humans. In order to establish a rat model of gastric banding which approximates the human procedure, unlike in previous models, 11,19 we developed a new model of gastric banding, as described below.…”