Modelo experimental de cultura primária de melanoma metastático por punção aspirativa de agulha fina

Oliveira, Aline Feitoza de; Gragnani, Alfredo; Filho, Renato Santos Oliveira; Santos, Ivan Dunshee de Abranches Oliveira; França, Silvana Gaiba de; Enokihara, Mílvia Maria Simões e Silva; Ferreira, Lydia Masako

doi:10.1590/s0102-86502005000500009

Cited by 2 publications

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References 6 publications

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“…Several factors contribute to its arising, such as a genetic predisposition, exposure to sunlight, physical traumas to pre-existent nevi and immunodeficiencies. Melanoma accounts for 3 to 4% of all malignant skin tumour [1][2][3][4] . Reports from various parts of the world have demonstrated an increase in its incidence and mortality rates 5,6 .…”

Section: Introductionmentioning

confidence: 99%

AZT on telomerase activity and cell proliferation in HS 839.T melanoma cells

Sobrinho¹,

Gragnani

Santos³

et al. 2012

Acta Cir. Bras.

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PURPOSE:To evaluate telomerase activity and proliferation of HS839.T melanoma cells, subjected to the action of AZT. METHODS:Cells were grown in triplicate, AZT at different concentrations: 50, 100 and 200μM, was added and left for 24 and 48 hours, and its effects were compared with the control group. Telomerase activity was detected by PCR and cell proliferation was evaluated by MTT. RESULTS:After 24 hours, there was no inhibition of cell proliferation or telomerase activity when compared to the control group. After 48 hours, there was a momentary decrease, suggesting that the cell lines used in this study are sensitive to AZT, but quickly recover both the enzyme activity and cell proliferation. CONCLUSION:The action of AZT on the melanoma cells studied, at the concentrations and times tested, did not inhibit telomerase activity nor affect cell proliferation.Key words: Telomerase. Melanoma. Zidovudine. Cell Proliferation. In Vitro.RESUMO OBJETIVO: Avaliar a atividade da telomerase e da proliferação de células de melanoma HS839.T submetidas à ação do AZT. MÉTODOS:As células foram cultivadas, em triplicata, com diferentes concentrações de AZT: 50, 100 e 200µM, por 24h e 48h, seus efeitos comparados com o grupo controle. A atividade da telomerase foi detectada por PCR e a proliferação celular avaliada por MTT. RESULTADOS:No tempo de 24 horas, não houve inibição da proliferação celular e da atividade da telomerase em comparação com o grupo controle. No período de 48 horas, houve uma diminuição momentânea, sugerindo que as células das linhagens utilizadas neste estudo são sensíveis ao AZT, mas que recuperam a atividade enzimática e proliferativa.CONCLUSÃO: Nas células de melanoma HS839.T estudadas e nas concentrações e tempos propostos, a ação do AZT não inibiu a atividade da telomerase e não afetou a proliferação celular.Descritores: Telomerase. Melanoma. Zidovudina. Proliferação de Células. In Vitro.Souza Sobrinho CP et al.

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Section: Introductionmentioning

confidence: 99%

AZT on telomerase activity and cell proliferation in HS 839.T melanoma cells

Sobrinho¹,

Gragnani

Santos³

et al. 2012

Acta Cir. Bras.

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Identification and quantification of notch receptors in human cutaneous melanoma using molecular biology techniques: literature review

et al. 2020

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Background The Notch signaling pathway and its modulators are directly related to growth, verticalization and metastasis in melanoma, being a possible therapeutic target for the treatment of this type of cancer. There are several methods of molecular biology to identify and quantify Notch receptors and it is essential to study them for understanding the different results, advantages and disadvantages of each. Main body The present study brings a bibliographic review on the molecular biology methods used in the identification and quantification of these molecules, aiming to facilitate research involving Notch receptors in human melanoma. We identified as main methods of molecular biology Western Blotting, Quantitative real-time polymerase chain reaction and DNA microarrays. A brief description of these methodologies is made and the advantages and disadvantages of each are discussed. Results concerning the function of this pathway are also discussed. Short conclusion It is known that the activation of Notch receptors is tumorigenic in most cases, however, depending on the microenvironment, it can provide tumor suppression. The adequate choice and use of the methodology for identification and quantification of Notch receptors is essential for the progress of knowledge of this important signaling pathway, which, certainly, will allow advances in the treatment of cutaneous melanoma.

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Modelo experimental de cultura primária de melanoma metastático por punção aspirativa de agulha fina

Cited by 2 publications

References 6 publications

AZT on telomerase activity and cell proliferation in HS 839.T melanoma cells

AZT on telomerase activity and cell proliferation in HS 839.T melanoma cells

Identification and quantification of notch receptors in human cutaneous melanoma using molecular biology techniques: literature review

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