Modelo de angiogênese inflamatória em córnea de coelho induzida pela cauterização alcalina pontual

Fechine-Jamacaru, Francisco Vagnaldo; Júnior, José Ueides Fechine; Filho, Manoel Odorico de Moraes

doi:10.1590/s0102-86502005000100010

Cited by 8 publications

(6 citation statements)

References 13 publications

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“…a neovascular nonspecific stimulus [13]. Prior to cauterization and all subsequent assessments, the animals were anesthetized with a combination of 12 mg/kg ketamine hydrochloride and 4 mg/kg xylazine hydrochloride i.m., complemented by 2 drops of 0.5% proxymetacaine hydrochloride applied topically to the conjunctival sac.…”

Section: Methodsmentioning

confidence: 99%

“…The angiogenesis response was measured by the Angiogenesis Quantifier System, a software developed specifically for this purpose [13]. The system processed the digital images of the cornea containing the area of angiogenesis and determined the following parameters: neovascularization area (NA), total vessel length (TVL) and blood vessel number (BVN) (fig.…”

Section: Methodsmentioning

confidence: 99%

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Potential Inhibitory Effect of LASSBio-596, a New Thalidomide Hybrid, on Inflammatory Corneal Angiogenesis in Rabbits

et al. 2012

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Aims: Evaluate the effect of LASSBio-596, structurally designed as a new hybrid of thalidomide, on inflammatory corneal angiogenesis. Methods: Eighteen rabbits were submitted to an alkaline cauterization in the right cornea. The animals were randomly allocated to three groups: vehicle, dexamethasone and LASSBio-596. Drugs were administered by eyedrops 3 times a day for 21 days. Evaluations were performed on days 3, 6, 9, 12, 15, 18 and 21 after cauterization. At these time points, digital images of the cornea were captured in a standard fashion. The angiogenic response was measured using software that was developed specifically for this purpose. It calculated the following parameters: neovascularization area (NA), total vascular length (TVL) and blood vessel number (BVN). Results: It was observed that dexamethasone significantly decreased NA, TVL and BVN during all assessments. From the NA the angiogenesis rate (AR) was calculated in each group. Therefore, dexamethasone completely inhibited the inflammatory corneal angiogenesis with an AR of –0.001 ± 0.006 mm²/day, which was significantly lower (p < 0.001) than that observed after treatment with vehicle (0.078 ± 0.024 mm²/day) and LASSBio-596 (0.054 ± 0.012 mm²/day). Although LASSBio-596 reduced angiogenesis in relation to vehicle, according to NA, TVL and BVN values, this difference was not statistically significant. However, it was found that the AR as measured in the LASSBio-596 group was significantly lower (p < 0.05) than that seen in control animals, indicating a potential antiangiogenic effect. Conclusion: We conclude that topical application of LASSBio-596 at 1.0% has a potential inhibitory effect on inflammatory corneal angiogenesis in rabbits.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Potential Inhibitory Effect of LASSBio-596, a New Thalidomide Hybrid, on Inflammatory Corneal Angiogenesis in Rabbits

et al. 2012

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“…The capacity to inhibit angiogenesis mediated by extract of C. echinata was evaluated using a model of inflammatory angiogenesis in the corneas of rats, according to the methodology described by Fechine-Jamacaru; Fechine Júnior; Moraes Filho 25, adapted for Wistar rats.…”

Section: Resultsmentioning

confidence: 99%

“…Shortly thereafter, the eye was rinsed with 10ml of 0.9% saline solution, to remove excess NaOH. This technique produced a circular, homogenous, well-defined cauterization site of about 3.5 mm in diameter 25.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Antioxidant and Antiangiogenic Properties of Caesalpinia Echinata Extracts

Gomes¹,

Jimenez²,

Silva³

et al. 2014

J. Cancer

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Natural products contain important combinations of ingredients, which may to some extent help to modulate the effects produced by oxidation substrates in biological systems. It is known that substances capable of modulating the action of these oxidants on tissue may be important allies in the control of neovascularization in pathological processes. The aim of this study was to evaluate the antioxidant and antiangiogenic properties of an ethanol extract of Caesalpinia echinata. The evaluation of antioxidant properties was tested using two methods (DPPH inhibition and sequestration of nitric oxide). The antiangiogenic properties were evaluated using the inflammatory angiogenesis model in the corneas of rats. The extract of C. echinata demonstrated a high capacity to inhibit free radicals, with IC50 equal to 42.404 µg/mL for the DPPH test and 234.2 µg/mL for nitric oxide. Moreover, it showed itself capable of inhibiting the inflammatory angiogenic response by 77.49%. These data suggest that biochemical components belonging to the extract of C. echinata interfere in mechanisms that control the angiogenic process, mediated by substrates belonging to the arachidonic acid cascade, although the data described above also suggest that the NO buffer may contribute to some extent to the reduction in the angiogenic response.

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“…We chose to evaluate the corneas at 14 days because angiogenesis has shown a rapid growth until the 12 th day in other models 14. In the previous studies, the vessel extent stabilized after this initial period.…”

Section: Discussionmentioning

confidence: 99%

The effect of subconjunctival bevacizumab on corneal neovascularization, inflammation and re-epithelization in a rabbit model

Mello

Pizzolatti²,

Wasilewski

et al. 2011

Clinics

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PURPOSE:To evaluate the use of subconjunctival bevacizumab on corneal neovascularization in an experimental rabbit model for its effect on vessel extension, inflammation, and corneal epithelialization.METHODS:In this prospective, randomized, blinded, experimental study, 20 rabbits were submitted to a chemical trauma with sodium hydroxide and subsequently divided into two groups. The experimental group received a subconjunctival injection of bevacizumab (0.15 m; 3.75 mg), and the control group received an injection of 0.15 ml saline solution. After 14 days, two blinded digital photograph analyses were conducted to evaluate the inflammation/diameter of the vessels according to pre-established criteria. A histopathological analysis of the cornea evaluated the state of the epithelium and the number of polymorphonuclear cells.RESULTS:A concordance analysis using Kappa's statistic showed a satisfactory level of agreement between the two blinded digital photography analyses. The neovascular vessel length was greater in the control group (p<0.01) than in the study group. However, the histopathological examination revealed no statistically significant differences between the groups in terms of the state of the epithelium and the number of polymorphonuclear cells.CONCLUSIONS:Subconjunctival bevacizumab inhibited neovascularization in the rabbit cornea. However, this drug was not effective at reducing inflammation. The drug did not induce persistent corneal epithelial defects.

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Modelo de angiogênese inflamatória em córnea de coelho induzida pela cauterização alcalina pontual

Abstract: The punctual alkaline cauterization of the rabbit cornea induces and sustains corneal neovascularization, so that, it can be used as model of angiogenesis.

Cited by 8 publications

References 13 publications

Potential Inhibitory Effect of LASSBio-596, a New Thalidomide Hybrid, on Inflammatory Corneal Angiogenesis in Rabbits

Potential Inhibitory Effect of LASSBio-596, a New Thalidomide Hybrid, on Inflammatory Corneal Angiogenesis in Rabbits

Evaluation of Antioxidant and Antiangiogenic Properties of Caesalpinia Echinata Extracts

The effect of subconjunctival bevacizumab on corneal neovascularization, inflammation and re-epithelization in a rabbit model

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