Adaptação do modelo experimental de colestase extra-hepática em ratos jovens

Abstract

Os autores descrevem a adaptação da técnica de ligadura e ressecção do ducto biliar comum descrita na literatura, enfatizando suas vantagens na recuperação pós-cirúrgica. Este trabalho foi realizado no Laboratório Experimental do Departamento de Pediatria da FMB-UNESP.

Comparative study between ligature with thread or metallic clamping by means of laparoscopy with the purpose of experimental biliary obstruction in swines

“…This fact, which is associated with the maximization of the image of the organs in video monitors, allows you to define the exact site of action, providing safe interventions to vital structures, even in complicated cases 28,29,44,45 . Experimental induction of extrahepatic cholestasis is a process that does not always succeed, requiring a high degree of technical quality, patience, concentration and continuous training 46,47 . But, seven days after the procedure for biliary obstruction, the animals showed strongly yellow urine, skin and mucous membranes icteric, ranging from mild to severe; stools lighter shade than that observed before ligation, so as to hepatomegaly palpation.…”

Water-soluble extract of Coleus barbatus modulates weight gain, energy utilization and lipid metabolism in secondary biliary cirrhosis: an experimental study in young rats

“…The ligature and resection of the common bile duct was performed according to the technique presented by Cameron and Oakley 3 with modifications 20 . The sham operation followed exactly the same technique described above with exception of the ligatures and common bile duct resection.…”