Tendências em experimentação animal

Monteiro, Rosângela; Brandau, Ricardo; Gomes, Walter J.; Braile, Domingo Marcolino

doi:10.1590/s0102-76382009000500012

Cited by 7 publications

(5 citation statements)

References 62 publications

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“…Researches in vivo are classified into four categories, according to the model of experimentation: 1) induced -situations where the condition/disease investigated is being experimentally induced; 2) spontaneous -uses genetic variants of human diseases that occur naturally in animals; 3) negative -uses animals, in which a specific disease does not develop or that demonstrate a lack of reactivity to a specific stimulus; 4) orphan -a condition which occurs naturally in animals under conditions not described in humans and later adopted when the disease is identified in humans (2)(3) .…”

Section: Experimental Research With Animal Modelsmentioning

confidence: 99%

“…Presupposes conducting pilot studies to identify potential problems, the exact use of test animals and their controls, obtaining as much information as possible about the study before its initiation, previous statistical prediction of the study, minimization of environmental variables such as disease, stress and diet that may affect the results and, finally, selecting the appropriate species for that purpose (4,11,13) . The animals used in research should meet ideal conditions, ie, must be kept in controlled environment to maintain the health and genetic parameters, to ensure uniformity in relation to environmental, genetic and experimental variables (2)(3) . The sample size can be estimated by mathematical relationship between the value of the studied effect (the biologically significant difference), the standard deviation (usually obtained from a pilot), the level of significance (usually between 0.05 and 0.01) and power of the test (usually between 80 and 90%) (14) .…”

Section: The Principles Of Experimental Research With Animal Modelsmentioning

confidence: 99%

“…In this scenario, the highlights are the treatments for coronary disease, cancer, diabetes, chronic degenerative diseases, vaccines and many others whose therapeutic advances, whether pharmacological or assistive, derived from animal models (3) . We highlight the need for the adoption of a humanized care with animal in research, and strategies to ensure animal welfare.…”

Section: Introductionmentioning

confidence: 99%

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Instrumental and ethical aspects of experimental research with animal models

Watanabe

Fonseca

Vattimo³

2014

Rev. esc. enferm. USP

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RESUmoOs modelos experimentais com animais oferecem possibilidades de conhecimento da fisiologia, da etiopatogenia das doenças e da ação de medicamentos que estão diretamente relacionados com a assistência de enfermagem de qualidade. Essa revisão integrativa descreve o estado atual dos aspectos instrumentais e éticos da pesquisa experimental com modelos animais, incluindo as principais recomendações dos comitês de ética que visam ao bem-estar animal e faz considerações sobre impacto de seus resultados na enfermagem assistencial. Os dados mostram que, no Brasil, o avanço na ética para o uso de animais para fins científicos consolidou-se com a Lei nº 11.794/2008, que estabelece os procedimentos éticos, atendendo ainda parâ-metros sanitários, genéticos e experimentais. A aplicação da ética no manuseio de animais para fins científicos e didáticos e a obtenção de dados consistentes e de qualidade trazem contribuições inquestionáveis ao enfermeiro, uma vez que oferecem subsídios para se relacionar mecanismos fisiopatológicos e a clínica do paciente.

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Section: Experimental Research With Animal Modelsmentioning

confidence: 99%

Section: The Principles Of Experimental Research With Animal Modelsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Instrumental and ethical aspects of experimental research with animal models

Watanabe

Fonseca

Vattimo³

2014

Rev. esc. enferm. USP

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“…A busca do entendimento de mecanismos e tratamento das doenças tem levado ao desenvolvimento de vários modelos animais de lesões ou outras patologias. Para que essas sejam investigadas a condição ou doença é induzida de forma experimental [6]. O uso do modelo animal permite superar limitações de estudos com humanos, como alguns aspectos éticos de experimentação em seres humanos e obtenção de número suficiente de casos com características homogêneas [7].…”

Section: Introductionunclassified

Avaliações funcionais e morfométrica de modelo de lesão muscular por agulha de biópsia em gastrocnêmio de ratos

et al. 2019

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Introdução: Dentre os modelos experimentais, o uso de agulha de biópsia mostrou ser eficaz para reproduzir as lesões em atletas. Objetivo: Avaliar a força de preensão, a nocicepção e as alterações morfológicas do músculo gastrocnêmio de animais submetidos à lesão muscular. Métodos: Foram utilizados seis ratos Wistar. A lesão foi realizada na junção miotendínea do músculo gastrocnêmio direito, utilizando agulha de biópsia. Para avaliação funcional utilizou-se o teste de força de preensão e avaliação nociceptiva em três momentos: 24 horas antes da lesão (AV1); no segundo dia pós-lesão (AV2) e no sexto dia pós-lesão (AV3). Ao final do experimento o músculo foi retirado para análise morfológica. Resultados: Houve redução da força de preensão entre AV1 e AV2 (p=0,0031). Para a nocicepção os valores de AV2 (p<0,001) e AV3 (p=0,0109) foram maiores que AV1. Na região das fibras musculares observou-se desarranjo do padrão fascicular, aumento do calibre de vasos sanguíneos, da quantidade de tecido conjuntivo, de fibroblastos e de células inflamatórias. Conclusão: A lesão muscular por agulha de biópsia provocou redução da força de preensão e da nocicepção do membro lesado, como também evidenciou as etapas características do processo de degeneração muscular e início do processo de regeneração.Palavras-chave: força, nociceptividade, músculo esquelético, biópsia por agulha.

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“…The domestic pig is still widely used as an animal model for the study of human disease, and has been shown to be similar to man for biological, anatomic, physiological and nutritional characteristics 10 . However, it has relatively high market value for use in scientific experimentation because of its maintenance.…”

Section: Introductionmentioning

confidence: 99%

Collared Pecary (tayassu tajacu) as a new model of renal ischemic injury induced by clamping the renal artery

et al. 2014

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PURPOSE:The use of the collared peccary as an experimental model for ischemic nephropathy. METHODS:A total of 12 collared peccary (Tayassu tajacu) was used and ischemic nephropathy was induced in six of these animals that constituted the experimental group (G1) while the other six formed the control group (G2). Ischemic nephropathy was induced surgically by partial occlusion of the left renal artery. The disease course was assessed by hematological tests, serum chemistry, urinalysis, ultrasound (US) and doppler ultrasound function of the renal artery before induction, and at five, 10, 15 and 20 days after surgery.Twenty days after the occlusion, unilateral nephrectomy and histopathological examination were performed to assess renal morphology. RESULTS:Statistical analysis by Fischer's test showed a significant difference (p<0.05) between the control group and the experimental group. The histopathological examination showed glomerular, tubular and interstitial lesions. In the experimental group, 83.3% (5 /6) showed moderate renal lesions and only 16.7% (1/6) were classified with no lesions. The ultrasound examination of the right kidney presented statistical difference between day 5 and day 10 post occlusion. CONCLUSION:The collared peccary as a good experimental model for ischemic renal disease, because it could be manipulated during the research time without death, with health conditions that permit any subsequent procedure for disease therapy.Key words: Artiodactyla. Kidney Diseases. Ischemia. Models, Animal. Collared Pecary (tayassu tajacu) as a new model of renal ischemic injury induced by clamping the renal arteryActa Cirúrgica 2014 -561

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Tendências em experimentação animal

Cited by 7 publications

References 62 publications

Instrumental and ethical aspects of experimental research with animal models

Instrumental and ethical aspects of experimental research with animal models

Avaliações funcionais e morfométrica de modelo de lesão muscular por agulha de biópsia em gastrocnêmio de ratos

Collared Pecary (tayassu tajacu) as a new model of renal ischemic injury induced by clamping the renal artery

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