Utilização do etanol na prevenção da calcificação em heteroenxerto valvar pulmonar porcino: estudo experimental em ovinos

The objective of this study was to evaluate the effect of chemical treatment with glutamic acid to avoid calcification of biological cardiac valves. The bovine pericardium (BP) tissues were fixed with 0.5% glutaraldehyde (BP/GA), followed by treatment with glutamic acid (BP/GA + Glu) for neutralization of the free aldehyde groups. Microscopic analysis showed that the wavy structure of collagen fibrils was preserved, but changes in elastin's integrity occurred. However, the treatment did not promote undesirable changes in the thermal and mechanical properties of the modified BPs. These samples were systematically studied in rat subcutaneous tissue: control (BP/GA) and anticalcificant (BP/GA + Glu). After 60 days, both groups induced similar inflammatory reactions. In terms of calcification, BP/GA + Glu remained more stable with a lower index (3.1 ± 0.2 μg Ca2+/mg dry tissue), whereas for BP/GA it was 5.7 ± 1.3 μg Ca2+/mg dry tissue. Bioprostheses made from BP/GA + Glu were implanted in the pulmonary position in sheep, and in vivo echocardiographic analyses revealed maintenance of valvar function after 180 days, with low gradients and minimal valve insufficiency. The explanted tissues of the BP/GA + Glu group had a lower average calcium content 3.8 ± 3.0 μg Ca2+/mg dry tissue. The results indicated high anticalcification efficiency of BP/GA + Glu in both subcutaneous implant in rats and in the experimental sheep model, which is an advantage that should encourage the industrial application of these materials for the manufacture of bioprostheses.

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In vivo evaluation of Vivere bovine pericardium valvular bioprosthesis with a new anti‐calcifying treatment

Braile-Sternieri

Góissis

Giglioti

et al. 2020

Artificial Organs

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Efeitos da radiação gama no comportamento mecânico e na calcificação do pericárdio bovino fixado com glutaraldeído

Baucia¹,

Neto²,

Rogero³

et al. 2007

Rev Bras Cir Cardiovasc

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Efeitos da radiação gama no comportamento mecânico e na calcificação do pericárdio bovino fixado com glutaraldeídoEffects of gamma irradiation on mechanical behavior and calcification of glutaraldehyde-fixed bovine pericardium Abstract Objective: To evaluate the effect of gamma irradiation on glutaraldehyde-fixed bovine pericardium.Method: Glutaraldehyde-fixed bovine pericardium was exposed to gamma radiation (doses from 0 to 10000 Gy). Six samples from each of nine groups were evaluated by optic microscopy, and shrinking and mechanical tests and the denaturation temperature was determined. Additionally, they were subcutaneously implanted in rats and after four months they were explanted and Ca 2+ levels measured by atomic absorption spectroscopy.Results: The Ca 2+ levels were (in µg/mg): control

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Utilização do etanol na prevenção da calcificação em heteroenxerto valvar pulmonar porcino: estudo experimental em ovinos

Cited by 2 publications

References 21 publications

In vivo evaluation of Vivere bovine pericardium valvular bioprosthesis with a new anti‐calcifying treatment

In vivo evaluation of Vivere bovine pericardium valvular bioprosthesis with a new anti‐calcifying treatment

Efeitos da radiação gama no comportamento mecânico e na calcificação do pericárdio bovino fixado com glutaraldeído

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