Estudo comparativo da eficácia do etanol e do ácido L-glutâmico na prevenção da calcificação das cúspides e parede aórtica porcina: estudo experimental em ratos

Rosa, George Ronald Soncini da; Costa, Francisco Diniz Affonso da; Mesquita, Lismari; Costa, Iseu de Santo Elias Affonso da

doi:10.1590/s0102-76382002000200009

Cited by 7 publications

(1 citation statement)

References 29 publications

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“…Therefore one of the approaches to reduce BHV calcification is focused on the neutralization of free aldehyde groups and the removal of glutaraldehyde residuals 12 . Procedures to minimize the problems attributed to GA processed BHV observed post implantation includes the treatment with amino acids 19 , particularly with Glu [20][21][22] , ethanol 23 , ethanol: Glu 24 , citric acid 25 and diamines 15 . From these treatments probably the first examples of a BHV developed under the concept of tissue engineering were those processed with GA followed by the treatment with Glu solutions in acid media that were characterized by low calcification levels associated with endothelial growth [20][21][22] .…”

Section: Introductionmentioning

confidence: 99%

Mechanical, thermal and morphological properties of glutaraldehyde crosslinked bovine pericardium followed by glutamic acid treatment

et al. 2009

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Major problems with valve bioprostheses are associated with progressive structural deterioration and calcification, directly associated with the use of glutaraldehyde (GA). This work describes the effects of GA processing and borate/glutamic acid buffer treatment on the mechanical, thermal and morphological properties of 0.5% GA crosslinked bovine pericardium (BP). The results showed that while the treatment of 0.5% GA crosslinked BP with borate/glutamic acid significantly improves the mechanical properties, it had no visible effect on surface morphology. Better surface preservation was only achieved for BP pre-treated with a lower GA concentration followed by the conventional treatment (0.5% GA). Improvements in mechanical properties probably arises from structural changes probably involving the depolymerization of polymeric GA crosslinks and an increase electrostatic interaction due to covalent binding of glutamic acid to free carbonyl groups (Schiff base). The results indicate that the treatment GA crosslinked BP with borate/glutamic acid buffer may be an attractive procedure for the manufacture of heart valve bioprostheses.

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Section: Introductionmentioning

confidence: 99%

Mechanical, thermal and morphological properties of glutaraldehyde crosslinked bovine pericardium followed by glutamic acid treatment

et al. 2009

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In vivo evaluation of Vivere bovine pericardium valvular bioprosthesis with a new anti‐calcifying treatment

et al. 2020

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The objective of this study was to evaluate the effect of chemical treatment with glutamic acid to avoid calcification of biological cardiac valves. The bovine pericardium (BP) tissues were fixed with 0.5% glutaraldehyde (BP/GA), followed by treatment with glutamic acid (BP/GA + Glu) for neutralization of the free aldehyde groups. Microscopic analysis showed that the wavy structure of collagen fibrils was preserved, but changes in elastin's integrity occurred. However, the treatment did not promote undesirable changes in the thermal and mechanical properties of the modified BPs. These samples were systematically studied in rat subcutaneous tissue: control (BP/GA) and anticalcificant (BP/GA + Glu). After 60 days, both groups induced similar inflammatory reactions. In terms of calcification, BP/GA + Glu remained more stable with a lower index (3.1 ± 0.2 μg Ca2+/mg dry tissue), whereas for BP/GA it was 5.7 ± 1.3 μg Ca2+/mg dry tissue. Bioprostheses made from BP/GA + Glu were implanted in the pulmonary position in sheep, and in vivo echocardiographic analyses revealed maintenance of valvar function after 180 days, with low gradients and minimal valve insufficiency. The explanted tissues of the BP/GA + Glu group had a lower average calcium content 3.8 ± 3.0 μg Ca2+/mg dry tissue. The results indicated high anticalcification efficiency of BP/GA + Glu in both subcutaneous implant in rats and in the experimental sheep model, which is an advantage that should encourage the industrial application of these materials for the manufacture of bioprostheses.

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In Vitro Properties and Performance of Glutaraldehyde‐Crosslinked Bovine Pericardial Bioprostheses Treated With Glutamic Acid

et al. 2011

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Calcification is one of the major causes of failure of heart valve bioprostheses (HVBs) derived from glutaraldehyde (GA)-processed bovine pericardium (BP) or porcine aortic valves. New crosslinking reagent procedures are still far from giving satisfactory results, and this is the main reason why GA is still the reagent of choice for the fixation of native tissue intended for HVB manufacture. Nevertheless, two new findings with respect to GA processing may significantly improve HVB performance postimplantation: the finding that increasing concentrations of GA result in a decrease in calcification; the blocking of free aldehyde usually by nucleophyles or the treatment of processed material at low pH. This work investigates the in vitro properties of BP fixed with GA followed by the treatment with glutamic acid under alkaline conditions in order to prepare BP materials with lower calcification potential postimplantation. In comparison to conventional processing, except for the tensile strength that was slightly lower, elongation and toughness were higher than the accepted values. No significant differences were observed in the performance indexes (mean pressure gradient, mean effective area, regurgitant fraction, performance and efficiency indexes) with wear resistance over 150 ¥ 10 6 cycles. These results indicate that the processing of BP described in this work may be of potential use in the manufacture of HVBs.

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Estudo comparativo da eficácia do etanol e do ácido L-glutâmico na prevenção da calcificação das cúspides e parede aórtica porcina: estudo experimental em ratos

Cited by 7 publications

References 29 publications

Mechanical, thermal and morphological properties of glutaraldehyde crosslinked bovine pericardium followed by glutamic acid treatment

Mechanical, thermal and morphological properties of glutaraldehyde crosslinked bovine pericardium followed by glutamic acid treatment

In vivo evaluation of Vivere bovine pericardium valvular bioprosthesis with a new anti‐calcifying treatment

In Vitro Properties and Performance of Glutaraldehyde‐Crosslinked Bovine Pericardial Bioprostheses Treated With Glutamic Acid

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