2013
DOI: 10.1590/s0102-695x2012005000111
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Development and validation of a HPLC method for the quantification of three flavonoids in a crude extract of Dimorphandra gardneriana

Abstract: A method for separation and quantification of three flavonoids by reverse-phase high performance liquid chromatography (HPLC) was developed and validated. Flavonoids present in a crude methanolic extract of the inner bark of Dimorphandra gardneriana Tul., Fabaceae, were analyzed. Rutin, isoquercitrin and quercetin were used as calibration standards. The analysis was performed using a Thermo Scientific Hypersil C18 column (250 x 4.0 mm i.d., 5 μm particle size), as stationary phase, with a flow rate of 0.8… Show more

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Cited by 21 publications
(9 citation statements)
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“…Therefore for the anhydrous form, their solubility in water is also diminished. Also, the existence of hydroxyl groups in C3-OH, C՜3-OH and C՜4-OH positions in conjunction with a C2, C3 double bond are responsible for the antioxidant property of quercetin [34,35]. The lower pH value has an important influence on the stability of quercetin.…”
Section: Resultsmentioning
confidence: 99%
“…Therefore for the anhydrous form, their solubility in water is also diminished. Also, the existence of hydroxyl groups in C3-OH, C՜3-OH and C՜4-OH positions in conjunction with a C2, C3 double bond are responsible for the antioxidant property of quercetin [34,35]. The lower pH value has an important influence on the stability of quercetin.…”
Section: Resultsmentioning
confidence: 99%
“…The UFLC analysis in FHMeOH of A. nutans leaves revealed that quercetin-3-Ogalactopyranoside is the identified phenolic compound which is the major constituent. The quantification by the UFLC-DAD method was validated and showed linearity, selectivity, and precision (BRASIL, 2003;DE AMORIM et al, 2014;LANDIM;FEITOZA;DA COSTA, 2013). From the Annonaceae family, reports including the quantitative determination of metabolites are uncommon, in particular for flavonoids (GARCÍA-SALAS et al, 2015), and most of the studies in the literature have evaluated the alkaloid and acetogenin content, because these two classes are the main ones in the species of the family (ALMEIDA, J. R. G. S.; JUNIOR, R. G. O; DE OLIVEIRA, 2015).…”
Section: Discussionmentioning
confidence: 99%
“…Analysis of the flavonoid contents was performed by HPLC using a previous method with minor modifications [29]. The peak areas of the active compound in each extract were directly compared with that of an authentic standard previously isolated in our laboratories.…”
Section: Chromatographic Conditionsmentioning
confidence: 99%