Development and validation of a HPLC method for the quantification of three flavonoids in a crude extract of Dimorphandra gardneriana

Abstract

A method for separation and quantification of three flavonoids by reverse-phase high performance liquid chromatography (HPLC) was developed and validated. Flavonoids present in a crude methanolic extract of the inner bark of Dimorphandra gardneriana Tul., Fabaceae, were analyzed. Rutin, isoquercitrin and quercetin were used as calibration standards. The analysis was performed using a Thermo Scientific Hypersil C18 column (250 x 4.0 mm i.d., 5 μm particle size), as stationary phase, with a flow rate of 0.8 mL/min and detection at a wavelength of 356 nm. The proposed method was validated by resolution RE No. 899/2003 of the National Health Surveillance Agency. In this study, an excellent linearity was obtained with r higher than 0.99. Besides, the chromatographic peaks showed good resolution. With other validation data, including precision, specificity, accuracy and robustness, this method demonstrated good reliability and sensitivity, and can be conveniently used for the quantification of rutin, isoquercitrin and quercetin in crude methanolic extract of D. gardneriana pods. Furthermore, there are the advantages of easy sample preparation and short time between each injection

Preparation of metal doped quercetin nanoparticles, characterization and their stability study

“…Therefore for the anhydrous form, their solubility in water is also diminished. Also, the existence of hydroxyl groups in C3-OH, C՜3-OH and C՜4-OH positions in conjunction with a C2, C3 double bond are responsible for the antioxidant property of quercetin [34,35]. The lower pH value has an important influence on the stability of quercetin.…”

Anti-inflammatory, antinociceptive and antioxidant activities of the hydromethanolic fraction from Annona nutans leaves

“…The UFLC analysis in FHMeOH of A. nutans leaves revealed that quercetin-3-Ogalactopyranoside is the identified phenolic compound which is the major constituent. The quantification by the UFLC-DAD method was validated and showed linearity, selectivity, and precision (BRASIL, 2003;DE AMORIM et al, 2014;LANDIM;FEITOZA;DA COSTA, 2013). From the Annonaceae family, reports including the quantitative determination of metabolites are uncommon, in particular for flavonoids (GARCÍA-SALAS et al, 2015), and most of the studies in the literature have evaluated the alkaloid and acetogenin content, because these two classes are the main ones in the species of the family (ALMEIDA, J. R. G. S.; JUNIOR, R. G. O; DE OLIVEIRA, 2015).…”

Extraction Optimization of 5,7-Dihydroxy-6,8,4′-trimethoxyflavonol, a Bioactive Flavonoid from Rubus rosifolius (Rosaceae) Leaves

“…Analysis of the flavonoid contents was performed by HPLC using a previous method with minor modifications [29]. The peak areas of the active compound in each extract were directly compared with that of an authentic standard previously isolated in our laboratories.…”