Bioactive compounds in Bidens pilosa L. populations: a key step in the standardization of phytopharmaceutical preparations

Cortés-Rojas, Diego Francisco; Chagas‐Paula, Daniela Aparecida; Costa, Fernando B. Da; Souza, C. R. F.; Oliveira, Wanderley Pereira de

doi:10.1590/s0102-695x2012005000100

Cited by 44 publications

(35 citation statements)

References 25 publications

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“…Among them, the main compounds are flavonoids and polyacetylenes, which are major anti-inflammatory phytochemicals in Bp [21,22]. In addition, both androgen and flavonoids are polyphenolic heterocyclic compounds.…”

Section: Western Blotmentioning

confidence: 99%

The Effect of the Aqueous Extract of Bidens Pilosa L. on Androgen Deficiency Dry Eye in Rats

Zhang

Yang

et al. 2016

Cell Physiol Biochem

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Background/Aims: Bidens pilosa L. (Bp) is widely distributed in China and has been widely used as a traditional Chinese medicine. The aim of this study was to examine the effect of the extract of Bp on androgen deficiency dry eye and determine its possible mechanisms. Methods: Twenty-four rats were randomly divided into four groups: Group Con (control), Group Sal (physiological saline), Group Fin (oral finasteride), and Group Bp (oral finasteride and Bp). The dry eye model was established in group Fin and group Bp. Aqueous tear quantity was measured with phenol red-impregnated cotton threads with anesthesia. Tear film breakup time (BUT) and corneal epithelial damage were evaluated by fluorescein staining. Animals were sacrificed at 28 days, and ocular tissues (lacrimal gland and cornea) were evaluated with light microscopy; gene microarray analysis for inflammatory cytokines and Western blot were also performed. Results: Finasteride administration effectively induced dry eye in rats by 14 days after administration. Group Fin rats had significantly higher fluorescein staining scores and lower aqueous tear quantity and BUT than the group Con rats, and notable inflammatory cell infiltrates were observed in the lacrimal gland of group Fin rats. The fluorescein staining score, aqueous tear quantity and BUT significantly improved with Bp treatment in the group Bp rats, and the structures of the lacrimal gland were well maintained without significant lymphocyte infiltration. Cytokine antibody array data identified the cytokines B7-2/Cd86, IL-1β, IL-4, IL-6, IL-10, MMP-8, FasL, TNF-α and TIMP-1 as candidates for validation by Western blot. Expression levels of pro-inflammatory cytokines, including IL-1β, IL-6, and TNF-α, in group Fin were upregulated compared with group Con. Levels of anti-inflammatory cytokines, such as IL-4 and IL-10, in group Fin were also upregulated compared with those in group Con. Compared with group Fin, IL-1β, FasL, and TNF-α were significantly decreased in group Bp. Conclusion: The extract of Bp appears to be effective for the treatment of androgen deficiency dry eye in rats by improving aqueous tear quantity, maintaining tear film stability, and inhibiting the inflammation of the lacrimal gland.

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Section: Western Blotmentioning

confidence: 99%

The Effect of the Aqueous Extract of Bidens Pilosa L. on Androgen Deficiency Dry Eye in Rats

Zhang

Yang

et al. 2016

Cell Physiol Biochem

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“…The percentage yield of crude flavonoid fraction extract from leaves of B. pilosa was 3% (30 mg/mL) which was higher compared to 1.4% from Boldo (Peumus boldus Molina) leaves of which the same method of extraction (Soxhlet) and solvents were used [24]. The 3% which was 30mg/mL flavonoid fraction extract from B. pilosa leaves was slightly higher as compared to the 2.01% extract of flavonoid as reported by Barua et al [25] and 19.623±0.1 mg/mL total flavonoid fraction extracted from B. pilosa L [26]. The differences in percentage yields of flavonoid fractions from B. pilosa observed could be due to different areas of growth for plant species.…”

Section: Percentage Yieldmentioning

confidence: 76%

Anti-bacterial Activity of Crude Flavonoid Fraction from Bidens pilosa Leaves against Selected Chronic Wound Bacterial Pathogens

Nakibuule¹,

Ntulume²,

Mwandah³

et al. 2019

JOCAMR

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Aims: Bidens pilosa is an extraordinary source of phytochemicals particularly flavonoids especially in leaves which have been attributed in various studies due to its antibacterial properties. The present study aimed at addressing bio-burden of chronic wound through proving a possible source of new antimicrobial product for wound healing. Methodology: Solvent-solvent extraction method was used to isolate crude flavonoid fraction from leaves of B. pilosa using ether, chloroform, ethylacetate and methanol (1:1:1). Thin-layer chromatography was used to identify crude flavonoid fraction using methanol/n-hexane (1:2: v/v) as mobile phase solvents. Agar well diffusion method was used to determine anti-bacterial activity of crude flavonoid against bacterial pathogens: Susceptible Pseudomonas aeruginosa ATCC®27853™, resistant Pseudomonas aeruginosa susceptible Staphylococcus aureus ATCC®25923™, methicillin resistant Staphylococcus aureus, Streptococcus pneumoniae and methicillin resistant Staphylococcus epidermidis. Minimum inhibitory concentration (MIC) and Minimum bactericidal contrition (MBC) were also determined using broth dilution and culture methods. Results: Thin-layer chromatographic profiling revealed an identity of three different spots with flavonoids from B. pilosa leaves showing three bands with Rf values 0.51, 0.60 and 0.63. The mean and standard deviation zone of inhibition of crude flavonoids ranged from 11.50±0.50 mm to 17.50±1.50 mm. The mean and standard deviation of positive controls (Ciproflaxacin, Co-Amoxiclay and Voncomycin) ranged from 0.00±0.00 to 22.50±0.50 mm. MIC and MBC of crude flavonoids ranged from 12.5-25.0 mg/mL and 50 to 200 mg/mL respectively. The flavonoid fraction was more effective against gram positive bacteria than on gram negative bacteria and it exhibited bactericidal effect on Methicillin resistant Staphylococcus aureus, resistant P. aureginosa, sensitive P.aureginosa and S. pneumonia. Conclusion: B. pilosa leaves could be a potential source for future drug development from flavonoid to address the issue of need for new antibiotics due to alarming burden of antimicrobial resistance in last resort antibiotics.

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“…Also the synergic effect of Ag and ZnO is beneficial for mass production of Reactive Oxygen Species such as H 2 O 2 , hence increased antimicrobial activity of nanocomposite [32,33]. Several nanoparticle synthesis methodologies have been employed including chemical methods [34], electrochemical methods [35], gamma radiation methods [36], photochemical [37] as well as laser ablation techniques [38]. A major impeding barrier as to employment of most of these conventional methods in nano-production is that they are cost prohibitive and also that some make use of harmful solvents and synthetic reactants which are environmental non-friendly [39][40][41][42][43][44][45][46][47].…”

Section: Introductionmentioning

confidence: 99%

Silver–zinc oxide nanocomposite antiseptic from the extract of Bidens pilosa

et al. 2019

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Silver nanoparticles (Ag-NPs), zinc oxide (ZnO-NPs) and zinc oxide-silver (ZnO-Ag-NPs) were biosynthesized based on the rich matrix of alkaloids, flavones, tannins capping/stabilizing agents present in Bidens pilosa extract. Different plant parts-root, leaf and seed ware used to prepare the plant extract for synthesis. Also, zinc and silver nitrate salts were used as precursor materials. The surface plasmon peaks (SPR) based on the UV-Vis results for the Ag-NPs, ZnO-NPs were located between 408-411 and 365-450 nm respectively. The SPR peaks for the Ag-ZnO-NPs occurred at 300-450 nm indicating both blue and red shifts. The Ag-ZnO-NPs SPR shifts were associated with possible nanoparticle size reduction and change in dielectric constant of the synthesis medium. Raman measurement peaks at 356, 484, 1350, 1578, 2435 cm −1 associated with OH,-C==C- ,-CO , S=O, =C-H moieties indicated successful capping. Nanoparticle yield was temperature dependent and optimal yield could not be tied to a particular plant part as source of extract. Tunneling electron microscope results showed Ag-NPs and ZnO-NPs were globular/spherical with a diameter range of 2-20 nm. Interestingly, ZnO-NPs TEM displayed isolated miniaturized globular nanoparticles (< 2 nm) which then joined up to form a large donut shaped structure indicating different formation mechanisms for the nanoparticles. XRD results showed the Ag-NPs, ZnO-NPs and the Ag-ZnO-NPs particles were crystalline in nature. The high signal/noise in XRD originated from possible crystalline biomaterials in the extracts. Energy dispersive spectroscopy (EDS) elemental composition results confirmed successful formation of the nanoparticles. Anti-Microbial activity of the synthesized Ag-NPs, ZnO-NPs and ZnO-Ag-NPs were studied against gram negative bacteria Escherichia coli (E. coli), gram positive bacteria Staphylococcus aureus and fungus Candida albicans. Different ZnO: Ag-NPs nanocomposite ratios were used to test for antimicrobial activity. Optimal antimicrobial activity was attained at Ag-NPs:ZnO-NPs ratio of 4:1 which also displayed the least minimum inhibition concentration (MIC) and therefore was used as the active ingredient in formulating a hand sanitizing antiseptic. The formulated antiseptic exhibited good antimicrobial activity.

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Bioactive compounds in Bidens pilosa L. populations: a key step in the standardization of phytopharmaceutical preparations

Cited by 44 publications

References 25 publications

The Effect of the Aqueous Extract of Bidens Pilosa L. on Androgen Deficiency Dry Eye in Rats

The Effect of the Aqueous Extract of Bidens Pilosa L. on Androgen Deficiency Dry Eye in Rats

Anti-bacterial Activity of Crude Flavonoid Fraction from Bidens pilosa Leaves against Selected Chronic Wound Bacterial Pathogens

Silver–zinc oxide nanocomposite antiseptic from the extract of Bidens pilosa

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