Sesame oil enhances endogenous antioxidants in ischemic myocardium of rat

Abstract

The present study was designed to evaluate the potency of antioxidant activity of sesame oil in-vitro model of myocardial ischemic reperfusion injury of rat. Sesame oil was administered orally to Wistar albino rats (180-200 g) in two different doses (n=6), by gastric gavage at a dose of 5 mL/kg b.w. (S1) and 10 mL/kg b.w (S2) daily for thirty days. Control and sesame oil treated rat hearts were subjected to invitro global ischemic reperfusion injury (5 min perfusion, 9 min nofl ow and 12 min reperfusion). A signifi cant rise in TBARS and decrease of GSH, catalase, LDH, CK and AST occurred in the hearts subjected to in-vitro myocardial ischemic reperfusion injury indicate the myocardial damage through oxidative stress. In sesame oil treated rats there was a signifi cant decrease in TBARS and signifi cant increase in endogenous antioxidants and myocardial marker enzymes in all the groups. In 10 mL/kg treatment group, a signifi cant rise in the levels of GSH, SOD and catalase were observed with marker enzymes, and it shows better recovery profi le than the other groups subjected to in-vitro ischemic reperfusion injury. In histological studies, control rats which subjected to IR injury show extensive myocardial damage and all the treatment groups, shows preserved myocardium. The effect of sesame oil was compared with reference compound captopril. The present study demonstrates that the sesame oil treated by the dose 10 mL/kg augments endogenous antioxidant compounds of the rat heart and also prevents the myocardium from in-vitro model of myocardial ischemic reperfusion injury.

Putative antioxidant property of sesame oil in an oxidative stress model of myocardial injury

“…In our previous study it was observed that the sesame oil shows protective role via enhancing the myocardial endogenous antioxidant system in the ischemic heart. 17 The same effect also observed in the present study could be an evidence for antioxidant mechanism of sesame oil as cardio protective agent.…”

“…Recently we found that chronic administration of sesame oil enhances the endogenous antioxidants in ischemic myocardium. 17 So, we hypothesized that the protective role of sesame oil against ISO induced myocardial injury may be via antioxidant system. Based on this hypothesis the present investigation is undertaken to find out the potency of antioxidant activity of chronic administration of sesame oil and to estimate the cardio protective action rendered by sesame oil.…”

Aqueous extract of Saussurea lappa root ameliorate oxidative myocardial injury induced by isoproterenol in rats

“…[3] AESL also offered significant inhibition activity in in-vitro antioxidant model which confirmed the protective role via the antioxidant mechanism. As per phytochemical investigation, the AESL contain flavonoids and phenolic compounds in high concentrations, which might be a responsible active principle for the cardio protective action.…”

“…According to the World Health Organization report, the maximum mortality occurs within the age limit of 40-50 years due to myocardial infarction (MI) in both western and developing countries. [123] Oxidative stress due to extensive generation of free radicals with concomitant depletion of endogenous antioxidants like superoxide dismutase, catalase and reduced glutathione (GSH) play an important role in MI. [3]…”

“…[123] Oxidative stress due to extensive generation of free radicals with concomitant depletion of endogenous antioxidants like superoxide dismutase, catalase and reduced glutathione (GSH) play an important role in MI. [3]…”