Anti-schistosomiasis triterpene glycoside from the Egyptian medicinal plant Asparagus stipularis

Abstract

Bioassay-guided isolation using an in vitro assay testing for antischistosomiasis yielded a novel triterpene saponin, asparagalin A, from the n-butanol extract of the roots of Asparagus stipularis Forssk., Asparagaceae. The structure was elucidated by spectroscopic analysis and chemical transformations. Administration of asparagalin A resulted in a retardation of worm growth and locomotion at the first day and showed a significant activity of egg-laying suppression at 200 µg/mL concentration.

Two new alkaloids from Asparagus stipularis Forssk. roots

“…Phytochemical investigations revealed the isolation of cytotoxic steroidal saponins (Shao et al, 1997;Kim et al, 2005;Zhou et al, 2007) and the alkaloid asparagamine A (Sekine et al, 1994). Previous investigations of Asparagus stipularis revealed the isolation of the steroidal saponintigogenin 3-O-dixyloglucoside (Halim et al, 1989) and the antischistosomiasis triterpene glycoside asparagalin A (El-Seedi et al, 2012). In the present study we report the isolation and structural elucidation of two new alkaloids, aspastipuline (1) and 5-hydroxyaspastipuline (2) from A. stipularis roots.…”

From Bench to Bedside: Natural Products and Analogs for the Treatment of Neglected Tropical Diseases (NTDs)

“…The triterpene saponin asparagalin A, from the n-butanol extract of the roots of Asparagus stipularis, showed a retardation of worm growth and a significant activity of egg-laying suppression at 200 mg/mL concentration [282].…”

Compounds from African Medicinal Plants with Activities Against Selected Parasitic Diseases: Schistosomiasis, Trypanosomiasis and Leishmaniasis

“…The anti-schistosomal activity of Asparagalin A ( 53 , Fig. 10 ), from the Egyptian medicinal plant Asparagus stipularis (Asparagaceae) has been evaluated [ 128 ]. It was found that this compound was able to significantly reduce the ability of adult female worms to lay eggs.…”

“… Trypanosomiasis and leishmanosomiasis (IC 50 = 5.7 μM). [ 127 ] 53 Terpenoid/Triterpenoid Roots Asparagus stipularis Asparagaceae Sinai, Egypt To treat Schistosomiasis (bilharziasis) amongst other ailments Schistosomiasis [ 128 ] 54 Terpenoid/Diterpenoid Root barks Elaeodendron schlechteranum Celastraceae Bunda district, Kung’ombe, Tanzania Treatment of anaemia, general body pain, dysmenorrhea, female infertility and male impotence, boils, carbuncles, cardiovascular problems including hypertension and joint inflammation. Trypanosomiasis ( T. cruzi (IC 50 < 0.57 μM), T. brucei (IC 50 < 0.57 μM) and leishmanosomiasis against ( L. infantum IC 50 = 1.67 μM) [ 129 ] 55 , 56 Roots Salacia madagascariensis Celastraceae Tanzania Treat malaria, fever, and menorrhagia Leishmanosomiasis [ 130 ] 57 , 58 Terpenoid/Diterpenoid and Triterpenoid Leaves Keetia leucantha (syn: Plectronia leucantha Krause ) Rubiaceae Benin To treat parasitic diseases Trypanosomiasis (IC 50 for 57 = 5.48 and 14.25 μM, respectively, on Tbb BSF and Tbb PF.…”