Anticholinesterase activity evaluation of alkaloids and coumarin from stems of Conchocarpus fontanesianus

Abstract

Abstract:Conchocarpus fontanesianus (A. St.-Hill.) Kallunki & Pirani, Rutaceae, popularly known as pitaguará, is a native and endemic tree from São Paulo and Rio de Janeiro States, Brazil. Based in the information that anticholinesterasic derivatives could act as new prototypes to treatment of Alzheimer disease, this work describes the fractionation guided by evaluation of the anticholinesterase activity of the ethanolic stems extract from C. fontanesianus. This procedure afforded the alkaloids dictamnine (1), γ-fagarine (2), skimianine (3), and 2-phenyl-1-methyl-4-quinolone (4), as well as the coumarin marmesin (5).

Furochinoline alkaloids in plants from Rutaceae family – a review

“…This alkaloid fraction was also analyzed via HPLC. In this work, in the stem material, the presence of dictamnine (1), skimmianine (2), γ-fagarine (3), 2-phenyl-1-methyl-quinoline and marmesine was confirmed [6].…”

“…St.-Hill.) Kallunki&Pirani, by Cabral et al [6], have been shown to have inhibitory activity towards acetyl cholinesterase. In this study, skimmianine (2) was shown to be more active.…”

“…The structures of the isolated alkaloids from the E. lepta are shown in Fig. 3 Cabral et al [6] utilized stem material of Conchocarpus fontanesianus (A. St.-Hill.)…”

“…The Rutaceae family has about 140 genera [6], consisting of herbs, shrubs and small trees which grow in all parts of the world [28,29,38], and which are used in traditional medicine for treating snake bites, stomatitis, rheumatism, bronchitis and other diseases [28]. This plant family is the source of furanocoumarines, furochinoline alkaloids, phenolic-structured compounds, terpens and other substances [12,25,27].…”

Establishment of an effective TLC bioautographic method for the detection ofMycobacterium tuberculosisH37Ra phosphoglucose isomerase inhibition by phosphoenolpyruvate

“…In our opinion, there is a clear trend towards the use of the TLC bioautography technique as a method for screening active compounds against microorganisms rather than a method for the detection of enzyme activities or inhibitors. The use of TLC (bio)autography was reported for the screening of inhibitors of acetylcholinesterase [12][13][14][15][16][17][18][19][20][21][22][23][24][25][26] , butyrylcholinesterase 12,16,22,23,27 , dipeptidyl peptidase IV 28 , glucose-6-phosphate dehydrogenase 29 , a-and b-glucosidases [30][31][32][33][34] , lipase [35][36][37] , monoamine oxidases A and B 38,39 , DNA topoisomerase I 40 , tyrosinase [41][42][43] and xanthine oxidase 44 . To date, the use of the TLC-based autographic method for the detection of PGI inhibition has only been developed for a biocatalyst isolated from the reference E. coli strain ATCC (American Type Culture Collection) 25922 45 .…”