Quality control and TLC profile data on selected plant species commonly found in the Brazilian market

Abstract

Abstract:The use of thin-layer chromatography (TLC) is a commonplace practice and can be of signifi cant help to different laboratories with quality control, especially those that work with plant extracts and phytotherapeutics. This study evaluated ten species of plants (Schinus terebinthifolius, Arctium lappa, Trichilia catigua, Camellia sinensis, Mikania glomerata, Croton moritibensis, Achyrocline satureioides, Heteropterys aphrodisiaca, Plantago major, Arctostaphylos uva-ursi) that are commonly sold by compounding pharmacies, using TLC with reference substances and pharmacopoeic physical and chemical tests (loss on drying, level of extractives, and total ash content). The results showed that the ten species showed losses on drying consonant with the literature. The level of extractives for two species and total ash for fi ve species were also consonant with the literature, and those of the other species were established in this study. The semipurifi ed extracts of the ten species were assayed by TLC, and the analysis with the use of reference substances proved to be effective, in addition to being practical, simple, versatile, and economically viable.

Evaluation of the Folin-Ciocalteu Method and Quantification of Total Tannins in Stem Barks and Pods from Libidibia ferrea (Mart. ex Tul) L. P. Queiroz

“…It is notable for its simplicity, low cost and speed, and it is worth noting the ease in which it can qualitatively detect the presence of compounds with the aid of reference standards 40 . To enable a more efficient separation process, the technological advances in the area of chromatography have led to the development of equipment that performs the application in a partially or totally automated way, ensuring volume reproducibility and homogeneity in this application 41,42 . The TLC analysis of samples revealed that stem bark and pods from L. ferrea presented a band with same coloration and displacement as that observed for the standard solution of gallic acid.…”

Phytochemical, Free Radical Scavenging Activity and Thin Layer Chromatography Analysis of Methanolic Extracts of Six Wild Mushroom Species Collected From the Shai Hills Reserve of Ghana

“…8 The TLC is also very useful for preliminary study before other instrumental techniques are applied. 9,10 The use of TLC for separation and purification of plant constituents depends on their size, shape and charge.…”

Chemical Fingerprints of an Indian Traditional Herbal Drug Talisapatra (Abies webbiana) and Comparison with English yew (Taxus baccata)

“…Application of HPTLC fingerprints in determination of quality of botanicals was explained by Nicoletti 40 . Braz et al 41 have used HPTLC technique to establish quality standards of selected plant species commonly found in the Brazilian market. HPLC is a popular method for the analysis of herbal medicines because of its easiness and HPLC analysis is not limited by the volatility or stability of the sample compound.…”