Analgesic effect of leaf extract from Ageratina glabrata in the hot plate test

P., Guadalupe García; S, Edgar García; G, Isabel Martínez; Scior, Thomas; Salvador, José L; P, Mauro M Martínez; Río, Rosa E. del

doi:10.1590/s0102-695x2011005000158

Cited by 14 publications

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“…The genus Ageratina (Asteraceae) consists of about 1200 species and is distributed in temperate and subtropical regions of the America, Europe, Africa, and Asia [ 1 , 2 , 3 , 4 ]; in Mexico, about 164 species of Ageratina have been reported [ 5 , 6 ]. Several species of this genus have been studied, and these studies have demonstrated bactericidal, antifungal, antiviral, analgesic, cytotoxic, and anti-inflammatory effects, as well as the ability to treat gastric ulcers [ 7 , 8 , 9 , 10 , 11 , 12 ]. In the state of Morelos, Mexico, Ageratina pichinchensis is traditionally used to treat gastric ulcers and heal deep wounds.…”

Section: Introductionmentioning

confidence: 99%

Establishment of a Cell Suspension Culture of Ageratina pichinchensis (Kunth) for the Improved Production of Anti-Inflammatory Compounds

Sánchez-Ramos

Álvarez

Romero-Estrada

et al. 2020

Plants

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Ageratina pichinchensis (Kunth) is a plant used in traditional Mexican medicine to treat multiple ailments. However, there have not been biotechnological studies on producing compounds in in vitro cultures. The aim of this study was to establish a cell suspension culture of A. pichinchensis, quantify the anti-inflammatory constituents 2,3-dihydrobenzofuran (2) and 3-epilupeol (3), evaluate the anti-inflammatory potential of its extracts, and perform a phytochemical analysis. Cell suspension cultures were established in a MS culture medium of 30-g L−1 sucrose, 1.0-mg L−1 α-naphthaleneacetic acid, and 0.1-mg L−1 6-furfurylaminopurine. The ethyl acetate extract of the cell culture analyzed by gas chromatography (GC) revealed that the maximum production of anti-inflammatory compounds 2 and 3 occurs on days eight and 16, respectively, improving the time and previously reported yields in callus cultures. The anti-inflammatory activity of these extracts exhibited a significant inhibition of nitric oxide (NO) production. Furthermore, a phytochemical study of the ethyl acetate (EtOAc) and methanol (MeOH) extracts from day 20 led to the identification of 17 known compounds. The structures of the compounds were assigned by an analysis of 1D and 2D NMR data and the remainder by GC–MS. This is the first report of the production of (-)-Artemesinol, (-)-Artemesinol glucoside, encecalin, and 3,5-diprenyl-acetophenone by a cell suspension culture of A. pichinchensis.

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Section: Introductionmentioning

confidence: 99%

Establishment of a Cell Suspension Culture of Ageratina pichinchensis (Kunth) for the Improved Production of Anti-Inflammatory Compounds

Sánchez-Ramos

Álvarez

Romero-Estrada

et al. 2020

Plants

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“…Oral administration of 300 and 500 mg/kg THME significantly ( F [1,14] Indomethacin-20 THME-150 THME-300 THME-500 Latency period, s Pretreatment Posttreatment Fig. 3.…”

Section: Hot Plate Testmentioning

confidence: 99%

“…Pain is a subjective experience resulting from the perception of an injurious stimulus and it includes an emotional component that requires the individual to be conscious when this is happening [1] . In 1986, the International Association for the Study of Pain (IASP) defined pain as an unpleasant sensory and emotional experience associated with actual or potential tissue damage, or described in terms of such damage [2] .…”

Section: Introductionmentioning

confidence: 99%

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Antinociceptive Activity of Methanol Extract of Tabebuia hypoleuca (C. Wright ex Sauvalle) Urb. Stems

Regalado

Mancebo

Paixão³

et al. 2017

Med Princ Pract

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than in the control group. In the formalin test, the licking time for THME at doses of 300 and 500 mg/kg was significantly shorter ( p < 0.001) compared to the control group in the first phase of the formalin test, whereas in the second phase only the dose of 500 mg/kg showed an antinociceptive effect. In addition, THME at doses of 300 and 500 mg/kg significantly increased the latency time in the tail flick test ( p < 0.05 and p < 0.001, respectively) and in the hot plate test ( p < 0.01 and p < 0.001, respectively) compared to the control group. Conclusions: These results show that THME had antinociceptive activity using several models of nociception, and they suggest that the effect is mediated by the participation of both peripheral and central antinociceptive mechanisms. AbstractObjective: The aim of this study was to evaluate the antinociceptive activity of the methanol extract of Tabebuia hypoleuca stems (THME). Materials and Methods: The animals were divided into 5 groups of 8 mice for each test (negative controls, positive controls, and 3 groups treated with THME at doses of 150, 300, and 500 mg/kg, p.o.). The antinociceptive effect of THME was evaluated using the writhing, formalin, tail flick, and hot plate models in mice. Results: In the writhing test, THME (150, 300, and 500 mg/kg) produced significantly ( p < 0.001) fewer writhes induced by acetic acid Significance of the Study• In this study Tabebuia hypoleuca was shown to have antinociceptive effects mediated by the participation of both peripheral and central antinociceptive mechanisms. The T. hypoleuca species could become a new therapeutic option for the treatment of pain.

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“…It is a disabling accompaniment which in many cases represents the only symptom for the diagnosis of several diseases. In relieve of pain, classical analgesic drugs notably opiates and non-steroidal anti-inflammatory drugs (NSAID) are used (García et al, 2011). The use of these drugs especially NSAID is considered to be the major risk factor in gastric ulcers.…”

Section: Introductionmentioning

confidence: 99%

Antinociceptive and antiulcer activities of Pycnanthus angolensis

Sofidiya

Awolesi

2015

Revista Brasileira de Farmacognosia

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a b s t r a c tPycnanthus angolensis (Welw) Warb., Myristicaceae, is used in Nigeria folk medicine to treat complaints such as toothache, headache, sore throat, ulcers and wounds. The aim of the study was to investigate the antinociceptive and antiulcer activities of the stem bark extract of Pycnanthus angolensis. Acute toxicity was conducted with a single oral dose of 5 g/kg. Antinociceptive activity was evaluated in acetic acid-induced writhing, formalin and tail immersion tests in mice while antiulcer activity was evaluated in ethanol and indomethacin-induced models in rats. In acetic acid-induced writhing test, the extract (50, 100 and 150 mg/kg, p.o.), significantly reduced the number of writhes (46.75%, 57.28% and 75.69%) respectively, compared to control. The extract significantly (p < 0.001) reduced the time spent in licking the hind paw at both phases, in formalin test. In tail immersion test, significant antinociceptive effect was only observed with the dose of 150 mg/kg, with peak effect at 90 min (43.38%). There is no significant change in the spontaneous locomotor activity of animals in the open field. The extract prevented the gastric ulceration caused by ethanol and indomethacin treatments compared to control. The results showed that P. angolensis extract possesses antinociceptive and antiulcer activities supporting the traditional use for relieving pain and ulcers.

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Analgesic effect of leaf extract from Ageratina glabrata in the hot plate test

Cited by 14 publications

References 14 publications

Establishment of a Cell Suspension Culture of Ageratina pichinchensis (Kunth) for the Improved Production of Anti-Inflammatory Compounds

Establishment of a Cell Suspension Culture of Ageratina pichinchensis (Kunth) for the Improved Production of Anti-Inflammatory Compounds

Antinociceptive Activity of Methanol Extract of Tabebuia hypoleuca (C. Wright ex Sauvalle) Urb. Stems

Antinociceptive and antiulcer activities of Pycnanthus angolensis

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