Antinociceptive Activity of Methanol Extract of Tabebuia hypoleuca (C. Wright ex Sauvalle) Urb. Stems

Regalado, Ada I.; Mancebo, Betty; Paixão, Armindo; López, Yanet; Merino, Nelsón; Sánchez, Luz María

doi:10.1159/000478015

Cited by 13 publications

(6 citation statements)

References 20 publications

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“…These results clearly demonstrated the therapeutic potential of P. schiedeana leaves against neurogenic pain, characteristic of an opioid analgesic 20,37 . On the other hand, the extracts were more effective in the second phase of the trial, reaching percentage inhibition values as high as 98%, even exceeding the 71% inhibition of the reference drug Table 1, suggesting a possible mechanism in the periphery, which again could be related to the reduction of the synthesis of prostaglandins, related to the nociceptive stimulus through local peritoneal receptors 38 ; or it may be due to interference in transduction mechanisms of the primary afferent nociceptors involved in this model. We can affirm that the evaluated extracts act in both phases during the formalin test, although with greater effectiveness in the phase of inflammatory pain.…”

Section: Resultsmentioning

confidence: 89%

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2021

IJPSR

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Traditionally Persea schiedeana is used for headache relief, as anti-hypertensive, nervous relaxant, dermal diseases, among others. In this study, this plant from Salvadorian flora acute toxicity was evaluated in-vivo at 500 mg/kg, and the analgesic activity was tested by acetic acid, formalin, and tail flick tests in NIH Swiss mice at doses of 100, 250, and 500 mg/kg body weight, using as a positive control Indomethacin 10 mg/kg and distilled water as a control. Secondary metabolite profile was performed by Thin Layer Chromatography (TLC) and ultra-efficient liquid chromatography-mass spectrometry (UPLC-MS). It was determined by TLC, the presence of terpenoids, flavonoids, tannins, lactonic groups, and coumarins, while UPLC-MS confirmed the presence of scopoletin. The biological assays demonstrated that P. schiedeana was deprived from toxic effect at the doses tested and the analgesic activity was confirmed in aqueous, ethanol, and dichloromethane extracts of Persea schiedeana leaves. Additionally, it was detected the presence of terpenoids, flavonoids, condensed tannins, lactones, and coumarins, and by UPLC-MS, the identity of coumarin as scopoletin was confirmed. These results are in accordance with the traditional use for pain relief. INTRODUCTION:Pain is a complex defense process, which indicates damage to a tissue, the sensitive perception of which is subjective, unpleasant, and with a large emotional component.

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Section: Resultsmentioning

confidence: 89%

Untitled

2021

IJPSR

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“…Group 5 administered 20 mg/kg of indomethacin used as the standard group. 21 Group 6 was given a combination of plant extract (400 mg/kg) and indomethacin (20 mg/kg). The reaction time (paw licking or jumping) was recorded for each mice after 30 min from the administration of the plant extract and standard groups.…”

Section: Hot Plate Methods Of Analgesiamentioning

confidence: 99%

Phytochemical and pharmacological studies of Lycium schweinfurthii methanolic leaves extract (Solanaceae) in mice

Sherif¹,

Bazine²,

Shlaka³

et al. 2022

PPIJ

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Lycium schweinfurthii is found in stony and sandy places belonging to the Solanaceae family which is widely distributed in North Africa and Mediterranean area. The leaves and fruits of this plant have traditionally been used for gastrointestinal diseases. This study was aimed to evaluate the neuropharmacological activity of the methanolic extract of Lycium schweinfurthii in experimental animals. The methanolic extract of the leaves was prepared by the fractionation technique. Male Albino mice of 22.2 ± 2.1 gm were used to evaluate the activity of the extract by Irwin primary screening test, diazepam and thiopental -induced sleep and open field apparatus for general locomotor activity as well as analgesic activity was evaluated by acetic acid-induced writhing and hot-plate. The phytochemical screening was performed by qualitative and quantitative analysis. Diazepam at 2 and 20 mg/kg, flumazenil at 2 mg/kg, thiopental at 40 mg/kg, diclofenac at 25 and 50 mg/kg and indomethacin at 20 mg/kg were all used as references. The selected dose of 400 mg/kg of the methanolic extract was selected after the Irwin screening test. Lycium schweinfurthii extract significantly prolonged the duration of diazepam-induced sleep without significant difference in the onset of sleep. Pretreatment with flumazenil completely blocked the hypnotic effects of the plant extract and diazepam. The plant extract with thiopental did not produce any significant change in the onset and duration time of sleep. In an open-field test, a combination of the plant extract with diazepam produced a significant reduction in both exploratory and general locomotor activity which was observed in four min and 30 min, respectively. The plant extract significantly induced a profound central and peripheral analgesic action. While, the phytochemical screening showed presences of flavonoids, tannins, saponins, phenols, cardiac glycoside, alkaloids and carbohydrates in the extract. The total flavonoids were the most content compounds in the extract. Thus, these findings indicate that Lycium schweinfurthii has depression, sedative and analgesic like activities.

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“…This test was performed using a tail flick apparatus (Pooya Armaghan Co., Tehran, Iran) to evaluate central analgesic effect [ 49 , 50 ]. The mice were placed horizontally in the mice enclosure, their tails (5 cm from the tip) exposed to the heat radiant, and the tail retraction time was recorded.…”

Section: Methodsmentioning

confidence: 99%

Discovery of a New Analgesic Peptide, Leptucin, from the Iranian Scorpion, Hemiscorpius lepturus

et al. 2021

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Hemiscorpius lepturus scorpion stings do not induce considerable pain based on epidemiological surveys conducted in the southwest part of Iran. Accordingly, this study was aimed to identify the analgesic molecule in H. lepturus venom by analyzing a cDNA library of the scorpion venom gland looking for sequences having homology with known animal venom analgesic peptides. The analgesic molecule is a cysteine rich peptide of 55 amino acids. the synthetic peptide was deprotected and refolded. RP-HPLC, Ellman’s, and DLS assays confirmed the refolding accuracy. Circular dichroism (CD) showed helix and beta sheet contents. This peptide, called leptucin, demonstrated 95% analgesic activity at the dose of 0.48 mg/kg in hot plate assay. Leptucin at the doses of 0.32, 0.48, and 0.64 mg/kg showed 100% activity in thermal tail flick test. No hemolysis or cytotoxicity was observed at 8 and 16 µg. Histopathology evaluations indicated no hepatotoxicity, nephrotoxicity, and cardiotoxicity. We thus report that leptucin is the analgesic agent of H. lepturus venom. Regarding the high in vivo efficacy of leptucin and the fact it shows no observable toxicity, it could be suggested as a drug lead in a preclinical study of acute pain as well as the study of its mechanism of action.

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Antinociceptive Activity of Methanol Extract of Tabebuia hypoleuca (C. Wright ex Sauvalle) Urb. Stems

Cited by 13 publications

References 20 publications

Untitled

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Phytochemical and pharmacological studies of Lycium schweinfurthii methanolic leaves extract (Solanaceae) in mice

Discovery of a New Analgesic Peptide, Leptucin, from the Iranian Scorpion, Hemiscorpius lepturus

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