Evaluation of antimalarial, free-radical-scavenging and insecticidal activities of Artemisia scoparia and A. Spicigera, Asteraceae

Afshar, Fariba Heshmati; Delazar, Abbas; Janneh, Omar; Nazemiyeh, Hossein; Pasdaran, Ardalan; Nahar, Lutfun; Sarker, Satyajit D.

doi:10.1590/s0102-695x2011005000144

Cited by 30 publications

(26 citation statements)

References 33 publications

(40 reference statements)

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“…The essential oils of A. scoparia showed strong insecticidal activity against stored-product insects. Similarly, the aerial parts of plant exhibited significant antimalarial, free radical scavenging, and insecticidal activities [16].…”

Section: Ulcersmentioning

confidence: 99%

Urease Inhibitory Activity of Aerial Parts of Artemisia scoparia: Exploration in an In Vitro Study

Khan

Tariq

et al. 2014

Ulcers

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Artimisia scoparia has been used in the treatment of different disorders including ulcers. The current study was therefore designed to investigate the aerial parts of Artemisia scoparia (crude extract, total sterol and flavonoidal contents, and aqueous fraction) for its urease inhibitory potential. The crude of the plant evoked marked attenuation on urease activity, when tested in various concentrations with IC50 values of 4.06 mg/ml. The inhibitory potential was further augmented in the aqueous fraction (IC50: 2.30 mg/ml) of the plant. When the total sterol and flavonoidal contents were challenged against urease, both showed concentration dependent activity; the latter showed maximum potency with IC50 values of 8.04 and 2.10 mg/ml, respectively. In short, the aerial parts of the plant demonstrated marked antagonism on urease and thus our study validated the traditional use of Artemisia scoparia in the treatment of ulcer.

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Section: Ulcersmentioning

confidence: 99%

Urease Inhibitory Activity of Aerial Parts of Artemisia scoparia: Exploration in an In Vitro Study

Khan

Tariq

et al. 2014

Ulcers

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“…Ao realizar o mesmo ensaio da redução do DPPH, Afshar et al (2011) De acordo com os resultados, quando se avalia a atividade antioxidante (AA) do extrato e frações das folhas, observa-se que comparado ao padrão rutina (considerando este padrão com 100% de AA) a FAE apresentou AA superior. Este resultado também é observado quando se analisa a FAE obtida das cascas do caule (Tabela 2).…”

Section: Resultado E Discussãounclassified

Atividade antioxidante e toxicidade preliminar do extrato e frações obtidas das folhas e cascas do caule de Dasyphyllum tomentosum (Spreng.) Cabrera

Paula

Canteli

Verdam

et al. 2014

Rev. bras. plantas med.

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RESUMO:Dasyphyllum tomentosum (Spreng.) Cabrera, açucará ou espinho-de-agulha, pertence à família Asteraceae, a qual compreende muitas espécies com propriedades terapêuticas. O objetivo deste trabalho foi avaliar extratos e frações de folhas e de cascas do caule de D. tomentosum, com relação as atividade antioxidante, citotóxica e hemolítica em testes in vitro. Todas as amostras apresentaram atividade antioxidante pelo método de inibição de DPPH, com destaque para a fração acetato de etila obtida das folhas cuja atividade foi comparável à dos padrões ácido ascórbico e rutina. Com relação à redução do complexo fosfomolibdênio, observou-se que esta mesma fração foi semelhante somente a rutina enquanto a fração obtida das cascas do caule apresentou resultado superior. Não foi observada atividade citotóxica e hemolítica frente aos modelos utilizados com os extratos e frações. Os resultados obtidos demonstram o potencial antioxidante da espécie sem apresentar toxicidade.Palavras-chave: antioxidante, DPPH, fosfomolibdênio, hemolítica, Asteraceae.ABSTRACT: Antioxidant activity and preliminary toxicity of the extracts and fractions obtained from the leaves and stem bark of Dasyphyllum tomentosum (Spreng.) Cabrera. Dasyphyllum tomentosum (Spreng.) Cabrera, which belongs to the Asteraceae family, is well known for having many species with therapeutic properties. The aim of this study was to evaluate the extracts and fractions from the leaves and stem bark of D. tomentosum with respect to the antioxidant, cytotoxic and hemolytic activity in in vitro tests. All samples showed antioxidant activity by the DPPH inhibition method, especially the ethyl acetate fraction obtained from the leaves, whose activity was comparable to that of standard ascorbic acid and rutin. Regarding the reduction of the phosphomolybdenum complex, we noted that this same fraction was only similar to rutin and the fraction obtained from the stem bark showed superior results. There was no cytotoxicity and hemolytic activity compared to the models used in the extracts and fractions. The results demonstrate the antioxidant potential of the species without showing toxicity.

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“…Then, in the combination therapy step, we observed that this extract particularly in a longer period of pretreatments, increased the sensitization of HeLa cells to radiation. Some of the biological components of Artemisia genus are coumarins, flavonoids, phenylpropanoids, glycosides, sterols and terpenoids, (28). Polyphenols, a group of compounds with high antioxidant activity, are rich in ethanolic and aqueous extracts.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic effects and radiosensitizing potential of Artemisia kopetdaghensis extract in human cervical cancel HeLa cells

Fanipakdel¹,

Hosseini²,

Afshar³

et al. 2019

Electron. physician

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Background: Cervical cancer is one of the most common causes of cancer death in women worldwide; Radiotherapy has a major role in cervical cancer treatment. Anti-cancer effects of other species of Artemisia have been shown in some human cancer cells. Objective: To determine the cytotoxic and radiosensitizing effects of Artemisia kopetdaghensis extract on cervical cancer HeLa cells. Methods: Different concentrations of Artemisia kopetdaghensis extract (ART) (25-250µg/ml) were examined on HeLa cell line. Cell cytotoxicity of the extract and combination of extract plus 2Gy radiation was evaluated after 24, 48 and 72 hours via MTT assay. ART induced apoptosis was estimated with flow cytometry after 24h. Oneway analysis of variance (ANOVA) and then Bonferroni post hoc test were applied for statistical analysis. Prism (v.6) was used for all statistical analyses. Results: Artemisia kopetdaghensis decreased HeLa cells viability according to its concentration and timing of treatment. Comparing with the control group, a sub-G1 peak in the flow cytometry histogram of A. kopetdaghensis treated cells was shown, demonstrating that apoptosis was involved in A. kopetdaghensis cytotoxicity. Also, A. kopetdaghensis extract combined with irradiation, induced an additive cytotoxic effect on HeLa cells. Conclusion: Artemisia kopetdaghensis extract might be considered as a radiosensitizer in cervical cancer treatment potentially, and can be a good candidate for future studies. Cells were irradiated with dose rate of 120 centigray (cGy)/min from a 60Co γ-irradiation source (Theratone780, Canada). Source to surface distance (SSD) was 80 cm. Radiation dose was 2 Gray (Gy) radiated with 9×5 field size. Preparation of extractA. kopetdaghensis shrubs were collected from Gonabad (Northeast Iran) and identified by the herbarium of Ferdowsi University of Mashhad, Iran (voucher specimen number: 35205). The herb was dried and the extraction carried out for 48 h with 70% ethanol using a Soxhlet apparatus. After drying the extract in water bath, it was dissolved in DMSO and was stored at -20 °c. Cell cultureThe cells were cultured in DMEM which contained FBS (10%) and penicillin/streptomycin (100 unit/mL) then kept in an incubator (O2: 90%, CO2: 95%, temperature: 37 °C). Determination of A. kopetdaghensis extract cytotoxicity by MTT assayThe cells were exposed to various doses of extract (25-250 µg/ml) for 24, 48 and 72 h. After the passing of these times, for assessing cell metabolic activity, MTT solution (5 mg/mL) was added to each well. The plates were

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Evaluation of antimalarial, free-radical-scavenging and insecticidal activities of Artemisia scoparia and A. Spicigera, Asteraceae

Cited by 30 publications

References 33 publications

Urease Inhibitory Activity of Aerial Parts of Artemisia scoparia: Exploration in an In Vitro Study

Urease Inhibitory Activity of Aerial Parts of Artemisia scoparia: Exploration in an In Vitro Study

Atividade antioxidante e toxicidade preliminar do extrato e frações obtidas das folhas e cascas do caule de Dasyphyllum tomentosum (Spreng.) Cabrera

Cytotoxic effects and radiosensitizing potential of Artemisia kopetdaghensis extract in human cervical cancel HeLa cells

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