Genotoxicity and cytotoxicity of Erythrina velutina Willd., Fabaceae, on the root meristem cells of Allium cepa

Silva, Deborah S.B.S.; Garcia, Anuska Conde Fagundes Soares; Mata, Samuel S.; Oliveira, Betejane de; Estevam, Charles S.; Scher, Ricardo; Pantaleão, S.M.

doi:10.1590/s0102-695x2011005000024

Cited by 14 publications

(5 citation statements)

References 24 publications

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“…De acordo com Parsons, Williams (2000) [29] os alcaloides, têm propriedades biológicas, incluindo a inibição da síntese de proteínas e DNA em certas células. Colocando-os assim como possíveis promotores da condensação dos cromossomos em fases do ciclo mitótico [30]. Em relação aos resultados do bioensaio com A. salina, foi possível mostrar que a infusão das folhas de S. purpurea apresentou toxicidade frente aos náuplios.…”

Section: Resultsunclassified

Avaliação da atividade genotóxica, citotóxica e antimicrobiana da infusão das folhas de Spondias purpurea L.

Santos

Marisco

2017

Sci. Plena

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_____________________________________________________________________________O uso de plantas medicinais cada vez mais é mostrado como uma fonte alternativa para tratamento de doenças. Este trabalho visou determinar o nível genotóxico e citotóxico da infusão das folhas de Spondias purpurea L., investigar o potencial antimicrobiano e conhecer o perfil cromatográfico de compostos presentes na infusão. Foram realizados os testes Allium cepa e Artemia salina para avaliar a genotoxicidade e citotoxicidade, respectivamente. Os métodos de difusão em disco e concentração inibitória mínima (CIM) foram usados para determinar a atividade antibacteriana nas bactérias Staphylococcus epidermidis, S. aureus, Escherichia coli, Salmonella enterica sorotipo Choleraesuis, Pseudomonas aeruginosa e Klebsiella pneumoniae. No teste de A. cepa, a análise estatística não indicou diferenças significativas do índice mitótico entre os tratamentos e o controle. O teste de A. salina mostrou que a infusão apresentou toxicidade frente aos náuplios a partir da dose 10µg/mL. Em relação à atividade antibacteriana, houve presença de halos de inibição contra as bactérias S. aureus e E. coli, e quando submetidos ao CIM, a infusão apresentou atividade antibacteriana contra todas as bactérias testadas. Sendo assim, a partir desse estudo pode-se inferir que a infusão (chá) das folhas da planta S. purpurea se mostrou como uma fonte medicinal não genotóxica para o teste A. cepa, bem como uma possível fonte antitumoral indicada pelo teste A. salina, além de uma promissora fonte antibacteriana.Palavras-chaves: Genotoxicidade, Antimicrobiana, Seriguela.The use of medicinal plants is increasingly shown as an alternative source for the treatment of diseases. This study aimed to determine the level genotoxic and cytotoxic infusion of the leaves of Spondias purpurea L., study the antimicrobial potential and know the chromatographic profile of the compounds present in the infusion. Tests were performed Allium cepa and Artemia salina to assess the genotoxicity and cytotoxicity, respectively. The disk diffusion method and the minimum inhibitory concentration (MIC) they were used to determine the antibacterial activity in bacteria Staphylococcus epidermidis, S. aureus, Escherichia coli, Salmonella enterica serotype Choleraesuis, Pseudomonas aeruginosa e Klebsiella pneumoniae. On test A. cepa, statistical analysis indicated no significant differences in mitotic index between treatment and control. The test of A. salina showed that infusion toxicity has opposite from the nauplii dose 10mg / ml. With regard to antibacterial activity, there was the presence of halos of inhibition against the bacteria S. aureus e E. coli, and when subjected to MIC infusion showed antibacterial activity against all tested bacteria. Thus, from this study can be inferred that the infusions (tea) from the plant leaves S. purpurea showed as a non-genotoxic medicinal source for testing A. cepa, as well as a possible antitumor source indicated by the test A. salina, as well as a promising anti...

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Section: Resultsunclassified

Avaliação da atividade genotóxica, citotóxica e antimicrobiana da infusão das folhas de Spondias purpurea L.

Santos

Marisco

2017

Sci. Plena

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“…marina banyak dimanfaatkan sebagai tanaman obat karena memiliki kandungan metabolit sekunder tetapi belum diketahui tingkat sitotoksik dan genotoksik A.marina terhadap sel dan kromosom. Hal ini perlu diketahui karena menurut (Silva et al, 2011) tumbuhan yang dapat digunakan sebagai tanaman obat dapat berpotensi toksik, karsinogenik atau teratogenik. Berdasarkan alasan tersebut maka perlu dilakukan uji genotoksisitas dan sitotoksisitas A.marina terhadap akar bawang dengan indikator indeks mitosis dan aberasi kromosom dengan menggunakan metode Allium assay.…”

Section: Pendahuluanunclassified

Sitotoksisitas dan Genotoksisitas Ekstrak N-Heksana dan Etanol Daun Api-Api (Avicennia marina (Forks.)Vierh) dengan Indikator Indeks Mitosis dan Aberasi Kromosom Akar Bawang (Allium cepa Linn.)

2022

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Avicennia marina merupakan tumbuhan mangrove memiliki senyawa metabolit sekunder yang dapat dimanfaatkan oleh masyarakan sebagai obat herbal,. Pengujian sitotoksisitas dan genotoksisitas perlu dilakukan untuk mengetahui toksisitas A. marina. Penelitian ini bertujuan untuk menguji sitotoksisitas dan genotoksisitas ekstrak n-heksana dan etanol mangrove A. marina. Penelitian dilakukan menggunakan metode eksperimental Rancangan Acak Lengkap (RAL), faktor tunggal dengan empat taraf konsentrasi yaitu: 125, 250, 500 dan 1000 ppm dan kontrol negatif larutan aquades serta kontrol positif larutan EMS, dengan 4 kali pengulangan. Parameter yang diamati adalah: indeks mitosis, pertumbuhan akar bawang dan aberasi kromosom. Hasil pengamatan dianalisis dengan ANAVA Ft(α.05), dan dilanjutkan dengan uji Duncan Ft(α.05). Ekstrak n-heksana daun A. marina bersifat sitotoksik dan genotoksik terhadap akar bawang pada konsentrasi 500 dan 1000 ppm, dengan konsentrasi 1000 ppm memberikan efek sub-letal. Ekstrak etanol daun A. marina bersifat sitotoksik dan genotoksik terhadap akar bawang, pada konsentrasi 500 dan 1000 ppm dan memberikan efek sub-letal. Aberasi kromosom yang didapatkan adalah break, bridge, double bridge, c-mitosis, delayed anaphase, diagonal anaphase, disoriented, giant cell, laggard, loss, mikronukleus, multipolar anaphase, polyploidy pada anaphase, ring, star, sticky, vagrant, lesion, dan double lesion. Avicennia marina is a mangrove plant that has secondary metabolites that can be used by the community as herbal medicine. Cytotoxicity and genotoxicity tests need to be carried out to determine the toxicity of A. marina. This study aimed to examine the cytotoxicity and genotoxicity of n-hexane and ethanol extracts of mangrove A. marina. The study was conducted using an experimental Completely Randomized Design (CRD) method, single factor with four concentration levels, namely: 125, 250, 500 and 1000 ppm and negative control is aquadest solution and positive control is EMS solution, with 4 times . Parameters observed were: mitotic index, onion root growth and chromosomal aberrations. The results of the observations were analyzed by ANOVA Ft(α.05), and continued with Duncan's Ft(α.05) test. The n-hexane extract of A. marina leaves was cytotoxic and genotoxic to onion roots at concentrations of 500 and 1000 ppm, with concentrations of 1000 ppm giving sublethal effects. The ethanolic extract of the leaves of A. marina was cytotoxic and genotoxic in onion roots, at concentrations of 500 and 1000 ppm and gave a sublethal effect. Chromosomal aberrations obtained were break, bridge, double bridge, c-mitosis, anaphase delay, diagonal anaphase, disorientation, giant cell, laggard, loss, micronucleus, multipolar anaphase, polyploidy at anaphase, ring, star, sticky, vagrant, lesion, and double lesion.

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“…However the study of Silva and coworkers (Silva et al, 2011) put in attention the use of this plant because through genotoxicity and cytotoxicity experiments on the root meristem cells of Allium cepa showed that the decoction of the leaves can have genotoxic effects at some doses and they recommend caution when using this plant for the preparation of teas and other medicinal products.…”

Section: Wwwintechopencommentioning

confidence: 99%

Erythrina, a Potential Source of Chemicals from the Neotropics

Soto-Hernández¹,

García-Mateos²,

Miguel-Chávez³

et al. 2012

Bioactive Compounds in Phytomedicine

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Genotoxicity and cytotoxicity of Erythrina velutina Willd., Fabaceae, on the root meristem cells of Allium cepa

Cited by 14 publications

References 24 publications

Avaliação da atividade genotóxica, citotóxica e antimicrobiana da infusão das folhas de Spondias purpurea L.

Avaliação da atividade genotóxica, citotóxica e antimicrobiana da infusão das folhas de Spondias purpurea L.

Sitotoksisitas dan Genotoksisitas Ekstrak N-Heksana dan Etanol Daun Api-Api (Avicennia marina (Forks.)Vierh) dengan Indikator Indeks Mitosis dan Aberasi Kromosom Akar Bawang (Allium cepa Linn.)

Erythrina, a Potential Source of Chemicals from the Neotropics

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