Cytotoxic and genotoxic activity of Achillea millefolium L., Asteraceae, aqueous extracts

Sousa, Saulo Marçal de; Viccini, Lyderson Facio

doi:10.1590/s0102-695x2011005000022

Cited by 15 publications

(10 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The presence of precocious chromosomes is thought to be due to unequal spindle movement where some chromosome arms are pulled towards the extremity of the pole (Staykova et al, 2005;Bhatta and Sakya, 2008). Lagging chromosomes have been a regular feature of many cytotoxicity/genotoxicity studies with medicinal plant extracts (Ozlem et al, 2008;Soliman, 2010;Sousa and Viccini, 2011). Sousa et al (2010) explained that such chromosomes have the potentials to form micronuclei.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the cytotoxicity and genotoxicity of aqueous leaf extracts of Azadirachta indica A. Juss using the Allium test

Akaneme¹

2012

J. Med. Plants Res.

View full text Add to dashboard Cite

The cytotoxicity and genotoxicity of aqueous leaf extracts of Azadirachta indica A. Juss were evaluated with the aid of the Allium test. The extract was found to exhibit mitoclassic and chromatoclassic effects. The abnormalities observed included: nuclear lesions, binucleate cells, prophase accumulation otherwise called prophase poison, sticky metaphase, star metaphase, metaphase c-banding, precocious chromosomes, lagging chromosomes, anaphase bridge, star anaphase, tripolar anaphase and sticky late anaphase. No abnormal cells were observed at telophase. The implications of these anomalies were also discussed. The results lend more support to the reports of several investigators on the urgent need to standardize the dosages of medicinal plant extracts for the treatment of various ailments.

show abstract

Section: Discussionmentioning

confidence: 99%

Evaluation of the cytotoxicity and genotoxicity of aqueous leaf extracts of Azadirachta indica A. Juss using the Allium test

Akaneme¹

2012

J. Med. Plants Res.

View full text Add to dashboard Cite

show abstract

“…The cytotoxic effects of plant extracts and essential oils can be evaluated through various cytogenetic assays, including the straightforward and relatively inexpensive root-tip assays (Sobita & Bhagirath 2005;Kuras et al 2006;Akinboro & Bakare 2007;Celik & Aslanturk 2007;Sousa et al 2009;Sousa & Viccini 2011). Application of plant-based genotoxicity assays is justifiable since the results attained using such systems generally correlate well with those obtained from analogous mammal-based tests (Celik & Aslanturk 2007).…”

Section: Introductionmentioning

confidence: 99%

“…In this context, Lactuca sativa L. (lettuce) is widely used in the detection of mutagens and genotoxic compounds in extracts derived from medicinal plants because it exhibits elevated sensitivity to exogenous agents. Furthermore, the L. sativa test enables various genetic endpoints to be verified, and allows the determination of mechanism of action of such agents against the DNA of the exposed organisms (Sousa & Viccini 2011).…”

Section: Introductionmentioning

confidence: 99%

Cytogenotoxic effects of ethanolic extracts of Annona crassiflora (Annonaceae)

et al. 2013

View full text Add to dashboard Cite

Infusions of the leaves and seeds of Annona crassiflora Mart. are commonly employed in the treatment of diarrhoea, snakebites, tumours and disorders of the hair and scalp. The aim of the present study was to investigate the cytotoxic and genotoxic properties of ethanolic extracts of A. crassiflora by evaluating their effects on germination, root elongation, chromosome structure and the cell division of Lactuca sativa (lettuce). The experiment followed a randomized design involving the treatment of L. sativa seeds with ethanolic extracts from leaves and seeds of A. crassiflora applied at ten concentrations (0.0125, 0.025, 0.05, 0.1, 0.2, 0.4, 0.6, 0.8, 1.0, and 1.2 mg/L) and with five repetitions per treatment. Seeds of L. sativa exposed for 48 h to A. crassiflora leaf extract at concentrations ≥ 0.1 mg/L, or to seed extracts at concentrations ≥ 0.2 mg/L, showed germination percentages that were significantly (p < 0.05) lower than those of seeds exposed to aqueous ethanol control. Exposure of L. sativa seedlings to leaf (but not seed) extracts of A. crassiflora produced significant (p < 0.05) reductions in the mitotic indices of root meristem cells of lettuce and induced chromosome and nuclear abnormalities in the root cells. The presence of chromosome stickiness, bridges, fragments, laggard chromosomes and nuclear condensation were also observed. The cytogenetic effects observed suggest that folkloric medicines prepared with extracts of the leaves or seeds of A. crassiflora should be employed with caution.

show abstract

“…These abnormalities show the direct effect of mutagens on the spindle apparatus and mostly cause somatic instability (Bhat et al 2007). Lagging chromosomes have been a regular feature of many cytotoxicity/ genotoxicity studies with medicinal plant extracts (Ozlem et al 2008, Haroun 2010, Sousa and Viccini 2011. Lagging chromosomes or fragments sometimes could disappear in the cytoplasm and thus be lost, giving rise to chromosome deletion, which is always lethal to humans (Hall and L. Mekki Cytologia 79(2) Garcia 2006).…”

Section: Cytogenetic Analysismentioning

confidence: 99%

Cytogenetic Effects of Crude Extracts of <i>Peganum harmala</i> Seeds and Their Effects on <i>Vicia faba</i> Plants

Mekki¹

2014

CYTOLOGIA

View full text Add to dashboard Cite

SummaryThe present study evaluates the genotoxic and cytotoxic effects of the crude water and ethanol extracts of Peganum harmala L. (Zygophyllaceae) on the Vicia faba L. M1 generation. Two types of treatments were carried out in this study. In the first type, the germinated root tips were treated with four dilutions of the water extract and ethanol extract (12.5, 25, 50 and 100%). All concentrations were applied for 3, 6, 12 and 24 h. In the second treatment, soaked V. faba seeds were treated with the same concentrations of the two extracts for 3, 6, 12, and 24 h, and then planted to raise the M1 generation. Root tips were squashed after Feulgen staining, and the prepared slides were analyzed for chromosomal aberrations and mitotic index changes. Significant concentrationdependent increase and duration-dependent decrease of MI were observed with aqueous extracts, while with ethanol extracts, the MI showed concentration-and duration-dependent decrease. The two extracts induced significant (p 0.01) increase in the percentage of nuclear and chromosomal aberrations. Concentration-and duration-dependent increase in the number of branches per plant and decrease in the other studied growth parameters were also recorded for all treatments with the water and ethanol extracts.

show abstract

Cytotoxic and genotoxic activity of Achillea millefolium L., Asteraceae, aqueous extracts

Cited by 15 publications

References 22 publications

Evaluation of the cytotoxicity and genotoxicity of aqueous leaf extracts of Azadirachta indica A. Juss using the Allium test

Evaluation of the cytotoxicity and genotoxicity of aqueous leaf extracts of Azadirachta indica A. Juss using the Allium test

Cytogenotoxic effects of ethanolic extracts of Annona crassiflora (Annonaceae)

Cytogenetic Effects of Crude Extracts of <i>Peganum harmala</i> Seeds and Their Effects on <i>Vicia faba</i> Plants

Contact Info

Product

Resources

About