Modulation of drug resistance in Staphylococcus aureus by extract of mango (Mangifera indica L., Anacardiaceae) peel

Abstract

Abstract:In an ongoing project to evaluate natural compounds isolated from by-products or wastes from vegetables and fruits (edible plants) as modulators of antibiotic resistance, ethanol extract from mango peel was investigated using Staphylococcus aureus strains possessing efflux mechanisms of resistance to norfloxacin, erythromycin and tetracycline. The minimum inhibitory concentrations (MIC) of the antibiotics were determined by the micro dilution assay in the absence and in the presence of sub-inhibitory mango peel extract concentration. Although the extract did not display relevant antibacterial activity (MIC≥2048 µg/mL), it modulated the activity of antibiotics, i.e. in combination with antibiotics (at 512 μg/mL), a four-fold reduction in the MIC values for tetracycline and erythromycin was observed. The results presented here indicates that mango peel could serve as a source of potential adjuvant of antibiotics which add value to this mango by-product.

Investigation of the Chemical Composition and Different Effects of a Rumex dentatus Methanol Extract Against Drug Resistant Pseudomonas aeruginosa Isolates

“…For example, Souto de Oliveira et al investigated the synergistic activity of norfloxacin, tetracycline, and erythromycin with an ethanol extract from Mangifera indica L. peel against S. aureus strains. The extract alone did not show significant antibacterial activity but a four-fold reduction in the MIC values was obtained for tetracycline and erythromycin when the extract was used in combination with these antibiotics (18). The investigation by Toroglu of the in-vitro synergistic effects of different herbs (Rosmarinus officinalis, Coriandrum sativum, Micromeria fruticosa L., Cumium cyminum, and Mentha piperita), when combined with gentamicin, cephalothin, ceftriaxone, and nystatin, showed that the combination of plant extracts with antibiotics resulted in lower drug resistance in 13 microbial species (19).…”

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“…In more details, P.Crispa extract (Arial parts) gave the widest spectrum of antibacterial potential against all studied isolates of S. aureus (MRSA) except S.aureus (2) with inhibition zone varying between 11-19 mm. In this direction, it is important to mention that the cell wall of S. aureus contains high amount of lipids which responsible usually for the high resistant behavior 19 .…”

Assessment of antimicrobial activity in vitro of ethanolic extracts of Banisteriopsis anisandra (A. Juss.) B. Gates (Malpighiaceae)

“…In addition to B. anisandra, ethanolic extracts from other medicinal species showed promising antibacterial activity against S. aureus, such as Lippia alba (Aguiar et al, 2008), Anacardium occidentale, Stryphnodendron adstringens, Bixa orellana, Eugenia uniflora L., Psidium guajava, Mimosa tenuiflora, Ilex paraguariensis, Ocotea odorífera, Hymenaea courbaril, and Schinus terebinthifolia (Gonçalves et al, 2005), and Cymbopogon citratus (Schuck et al, 2001). Species tested whose leaves showed no inhibitory activity included Genipa americana, Tabebuia avellanedae, Casearia sylvestris, Pterodon emarginatus, Copaifera langsdorffii., Anadenanthera colubrine, and Myroxylon peruiferums (Gonçalves et al, 2005) and mango peels (Oliveira et al, 2011).…”