Avaliação da atividade mutagênica e antimutagênica da Aloe vera em teste de Allium cepa e teste de micronúcleo em linfócitos humanos binucleados

Sturbelle, R.T.; Pinho, Daiane S. de; Restani, Rossana G.; Oliveira, Gisele Rodrigues de Carvalho; Garcias, Gilberto de Lima; Martino-Roth, Maria da Graça

doi:10.1590/s0102-695x2010000300019

Cited by 18 publications

(17 citation statements)

References 11 publications

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“…For the treatments using the aqueous extracts prepared with P. longipes leaves, significantly lower mitotic indexes for both concentrations and samples were observed compared with the negative control, confirming that the plant extract exhibited anti-proliferative potential. The same effect was found by Sturbelle et al (2010) in a study using the onion test to assess the concentrations of babosa solution (Aloe vera L.), 40 and 400 mL L -1 , whose results showed inhibition of cell division following application of the solutions on meristematic cells of onion.Given the fact that the extracts studied were from plants collected from different cities, the results of treatments at the same concentration involving different samples differed significantly in mitotic index. By contrast, treatments with extracts from the same sample, even at different concentrations, showed similar effects on the cellular division of A. cepa.…”

supporting

confidence: 75%

Genotoxic and chromatographic analyses of aqueous extracts of Peltodon longipes Kunth ex Benth. (hortelã-do-campo)

Kuhn

Tedesco

Boligon

et al. 2015

Braz. J. Pharm. Sci.

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Peltodon longipes is used as a stimulant and emmenagogue. The objective of this study was to perform genotoxic and chromatographic analyses of the extracts of two samples of P. longipes, collected from the cities of Santa Maria and Tupanciretã, RS, Brazil. The Allium cepa assay was used to analyze genotoxicity while high-performance liquid chromatography was employed to determine phenolic compounds. The genotoxicity experiment consisted of nine groups each comprising four A. cepa bulbs. Bulb roots were developed in distilled water and then transferred for the treatments, for 24 hours, and the negative control remained in water. The treatments were: aqueous extracts at concentrations of 5 and 15 g L -1 for each sample, plus four groups treated with 1% glyphosate, one of which was used as a positive control and the other three for testing DNA damage recovery using water and the extracts of P. longipes from Santa Maria. All extracts of P. longipes exhibited anti-proliferative potential, although the effect was significantly greater for the extracts from the Tupanciretã sample. This sample also contained the highest amount of rosmarinic acid and kaempferol, which may confer the effects found in these extracts. Only extracts from the Santa Maria sample exhibited genotoxic potential.Uniterms: P. longipes/antiproliferative effect. P. longipes/genotoxic potential. P. longipes/chromatographic analyses. Allium cepa test/genotoxicity.Peltodon longipes é utilizada como estimulante e emenagoga. Objetivou-se realizar análises genotóxica e cromatográfica dos extratos de duas amostras de P. longipes, coletadas nos municípios de Santa Maria e Tupanciretã, RS, Brasil. O teste de Allium cepa foi utilizado para análise da genotoxicidade e a cromatografia líquida de alta eficiência, para determinação dos compostos fenólicos. O experimento de genotoxicidade constou de nove grupos de quatro bulbos de A. cepa. Os bulbos foram enraizados em água destilada e após transferidos para os tratamentos, por 24 horas, permanecendo o controle negativo em água. Os tratamentos foram: extratos aquosos nas concentrações de 5 e 15 g L -1 de cada amostra, além de quatro grupos tratados com glifosato 1%, um deles usado como controle positivo e outros três para testar a recuperação de danos ao DNA, utilizando água e os extratos de P. longipes da amostra de Santa Maria. Todos os extratos de P. longipes demonstraram potencial antiproliferativo, porém o efeito foi significativamente maior para os extratos da amostra de Tupanciretã. Essa amostra também apresentou maior quantidade de ácido rosmarínico e canferol, o que pode estar relacionado com os efeitos encontrados nesses extratos. Somente extratos da amostra de Santa Maria demonstraram potencial genotóxico. Unitermos INTRODUCTIONThe species Peltodon longipes Kunth ex Benth., belonging to the Lamiaceae family, is found in the Southern region of Brazil and is also referred to as P. comaroides Briq. (Briquet, 1989). The plant is known popularly as hortelã-do-campo (wild mint) (Lorenzi, Matos, 2008) and is u...

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supporting

confidence: 75%

Genotoxic and chromatographic analyses of aqueous extracts of Peltodon longipes Kunth ex Benth. (hortelã-do-campo)

Kuhn

Tedesco

Boligon

et al. 2015

Braz. J. Pharm. Sci.

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“…Para cada concentração da infusão foram dispostos quatro bulbos de A. cepa seguindo a metodologia proposta por Sturbelle et al (2010) [22]. Foram analisadas 3500 células de cada bulbo, totalizando 14000 células por tratamento.…”

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Avaliação da atividade genotóxica, citotóxica e antimicrobiana da infusão das folhas de Spondias purpurea L.

Santos

Marisco

2017

Sci. Plena

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_____________________________________________________________________________O uso de plantas medicinais cada vez mais é mostrado como uma fonte alternativa para tratamento de doenças. Este trabalho visou determinar o nível genotóxico e citotóxico da infusão das folhas de Spondias purpurea L., investigar o potencial antimicrobiano e conhecer o perfil cromatográfico de compostos presentes na infusão. Foram realizados os testes Allium cepa e Artemia salina para avaliar a genotoxicidade e citotoxicidade, respectivamente. Os métodos de difusão em disco e concentração inibitória mínima (CIM) foram usados para determinar a atividade antibacteriana nas bactérias Staphylococcus epidermidis, S. aureus, Escherichia coli, Salmonella enterica sorotipo Choleraesuis, Pseudomonas aeruginosa e Klebsiella pneumoniae. No teste de A. cepa, a análise estatística não indicou diferenças significativas do índice mitótico entre os tratamentos e o controle. O teste de A. salina mostrou que a infusão apresentou toxicidade frente aos náuplios a partir da dose 10µg/mL. Em relação à atividade antibacteriana, houve presença de halos de inibição contra as bactérias S. aureus e E. coli, e quando submetidos ao CIM, a infusão apresentou atividade antibacteriana contra todas as bactérias testadas. Sendo assim, a partir desse estudo pode-se inferir que a infusão (chá) das folhas da planta S. purpurea se mostrou como uma fonte medicinal não genotóxica para o teste A. cepa, bem como uma possível fonte antitumoral indicada pelo teste A. salina, além de uma promissora fonte antibacteriana.Palavras-chaves: Genotoxicidade, Antimicrobiana, Seriguela.The use of medicinal plants is increasingly shown as an alternative source for the treatment of diseases. This study aimed to determine the level genotoxic and cytotoxic infusion of the leaves of Spondias purpurea L., study the antimicrobial potential and know the chromatographic profile of the compounds present in the infusion. Tests were performed Allium cepa and Artemia salina to assess the genotoxicity and cytotoxicity, respectively. The disk diffusion method and the minimum inhibitory concentration (MIC) they were used to determine the antibacterial activity in bacteria Staphylococcus epidermidis, S. aureus, Escherichia coli, Salmonella enterica serotype Choleraesuis, Pseudomonas aeruginosa e Klebsiella pneumoniae. On test A. cepa, statistical analysis indicated no significant differences in mitotic index between treatment and control. The test of A. salina showed that infusion toxicity has opposite from the nauplii dose 10mg / ml. With regard to antibacterial activity, there was the presence of halos of inhibition against the bacteria S. aureus e E. coli, and when subjected to MIC infusion showed antibacterial activity against all tested bacteria. Thus, from this study can be inferred that the infusions (tea) from the plant leaves S. purpurea showed as a non-genotoxic medicinal source for testing A. cepa, as well as a possible antitumor source indicated by the test A. salina, as well as a promising anti...

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“…In a study developed by Sturbelle et al (2010), Aloe vera (L.) Burm. f. solutions were tested using the Allium cepa test to determine the level of antimutagenicity of this plant.…”

mentioning

confidence: 99%

Assessment of the antiproliferative and antigenotoxic activity and phytochemical screening of aqueous extracts of Sambucus australis Cham. & Schltdl. (ADOXACEAE)

Tedesco

Kuhn

Frescura

et al. 2017

An. Acad. Bras. Ciênc.

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The purpose of this study was to evaluate the antiproliferative and antigenotoxic activity of Sambucus australis Cham. & Schltdl. aqueous extracts on the cell cycle of Allium cepa L. as well as determine the phenolic compounds in such extracts. S. australis inflorescences and leaves of two accessions were used for aqueous extract preparation at concentrations: 0.003 g/ml and 0.012 g/ml. A. cepa bulbs were rooted in distilled water and, subsequently, placed in treatments for 24 hours. Rootlets were collected and fixed in modified Carnoy's solution for 24 hours and kept. The squash technique was performed for slide preparation. Root tips were smashed and stained with 2% acetic orcein, and a total of 4000 cells per treatment were analyzed. The phenolic compounds were determined using high-performance liquid chromatography and data was analyzed using the Scott-Knott test. The results show that S. australis aqueous extracts have antiproliferative potential. Besides, the extracts prepared from S. australis leaves of both accessions at a concentration of 0.012 g/ml have shown antigenotoxic activity. The phytochemical analysis allowed us to determine the presence of flavonoids and phenolic acids, of which kaempferol and chrologenic acid were the most predominant compounds in the extracts from the inflorescences and leaves, respectively.

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Avaliação da atividade mutagênica e antimutagênica da Aloe vera em teste de Allium cepa e teste de micronúcleo em linfócitos humanos binucleados

Cited by 18 publications

References 11 publications

Genotoxic and chromatographic analyses of aqueous extracts of Peltodon longipes Kunth ex Benth. (hortelã-do-campo)

Genotoxic and chromatographic analyses of aqueous extracts of Peltodon longipes Kunth ex Benth. (hortelã-do-campo)

Avaliação da atividade genotóxica, citotóxica e antimicrobiana da infusão das folhas de Spondias purpurea L.

Assessment of the antiproliferative and antigenotoxic activity and phytochemical screening of aqueous extracts of Sambucus australis Cham. & Schltdl. (ADOXACEAE)

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