Development of a new propolis microemulsion system for topical applications

Abstract

RESUMO: "Desenvolvimento de um novo sistema microemulsionado de própolis para aplicação tópica". Sistemas microemulsionados (MES) oferecem inúmeras vantagens como liberadores de fármacos já que favorecem a absorção do princípio ativo e são, em muitos casos, mais eficientes do que outros métodos de liberação de substâncias bioativas. Neste trabalho, um MES foi obtido visando uma formulação para uso tópico, contendo extrato etanólico de própolis (PEE). Neste estudo, Diagramas de fase pseudoternários foram utilizados para caracterização das regiões de microemulsão, bem como para definição da região de Winsor IV do sistema PEE-MES (Tween 80 como tensoativo e álcool etílico como co-tensoativo, em baixo percentual). Os resultados obtidos indicaram que o melhor sistema microemulsionado contém C/S (cotensoativo/tensoativo) na razão 1,0, fornecendo uma microemulsão do tipo O/A com ampla região de Winsor IV. A formulação PEE-MES poderá vir a ser utilizada comercialmente para uso tópico no combate a inflamações de boca e garganta. Considerando o amplo uso de própolis em formulações convencionais, onde sua solubilidade representa um dos maiores problemas, o sistema PEE-MES disponibiliza este produto natural para aplicações farmacológicas, com boa solubilidade em um sistema microemulsionado do tipo O/A. Adicionalmente, o efeito bactericida do sistema PEE-MES foi comprovado e uma nova metodologia de extração de própolis é apresentada.Unitermos: Própolis, sistema microemulsionado, formulação tópica.ABSTRACT: Microemulsion systems (MES) offer advantages as drug delivery systems, among them favour drug absorption, being in most case more efficient than other methods in delivering of drug. In this work a new MES was obtained in order to be applied as a pressurized aerosol formulation containing bee propolis ethanolic extract (PEE). For that, pseudoternary phase diagrams were used to characterize the microemulsions boundaries and also to define the Winsor IV microemulsion region of the PEE-MES system containing Tween 80 as surfactant and the cosurfactant ethyl alcohol in small percentage. The obtained results indicated that the best MES was composed by Tween 80 and ethyl alcohol with C/S (cosurfactant/surfactant) ratio equal to 1.0, since it provided a large boundaries in the obtained O/W microemulsion region. This PEE-MES formulation, in which bee propolis consisting as oil phase, is herein designed for topical uses (PEE-MES spraying) in order to treat mouth and throat inflammatory infections. Considering the very large uses of bee propolis in conventional vehicles, MES type of delivery system has to be compatible with achieving the highest drug aim loadings, determined substantially by the specific MES application (drug solubilization in water systems) improving in this case, propolis farmacological aplications. Additionally, PEE-MES antibacterial effect was evidenced and the microemulsion system PEE-MES was also used as newest chemical approach for extraction of bee propolis material from resinous hive.

Efecto de recubrimiento de almidón de yuca modificado y aceite de tomillo aplicado al pimiento (Capsicum annuum)

“…Inputs thyme essential oil with purity of 99% as an antimicrobial agent; glycerin with purity of 99.9% as plasticizer (Amaya et al, 2010. ) Tween 80 which confers stability to the emulsions and improve coating adhesion (Dantas and Silva, 2010).…”

Analytical methods applied to diverse types of Brazilian propolis

“…A propolis sample from the north of Brazil was extracted with ethanol and incorporated in emulsions containing different compositions of Tween 20, Tween 80, alcohol and water. The microemulsions were active against gram positive bacteria [ 16 ].…”

Physicochemical Characterizations and Antioxidant Property of Copaiba Oil Loaded into SNEDDS Systems

“…According to this methodology, the mixture of several compounds with different physicochemical properties can generate various colloidal systems including spontaneous monophasic systems. 13,[16][17][18][19][20][21][42][43][44][45][46][47][48] The ternary phase diagrams of SNECO, SNECO-COO and SNECO-SO were obtained by using Tween 80, oil phase and water. Specifically, the samples were formulated by using 20% of surfactant (Tween 80), 1% of oil phase based on CO, and 79% of double-distilled water, affording o/w colloidal systems obtained without cosurfactant need.…”